FastQCFastQC Report
Mon 15 Aug 2016
SRR2921073_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921073_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2773076
Sequences flagged as poor quality0
Sequence length125
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA65000.23439674931375842No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30810.1111040591747215No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA103250.033.0485271
GGTATCA72700.030.0325951
AACGCAG112000.029.8620996
GTACATG140000.029.6143991
TCAACGC115450.028.9228784
TACATGG141450.028.73922
CATGGGG85400.028.157634
CAACGCA120150.027.8410195
TATCAAC121500.027.8246042
ATCAACG123350.027.1670113
ACGCAGA123150.026.8664957
ACATGGG149800.026.3832863
CGCAGAG127050.025.853978
GTATAGT6950.024.8919751
GCAGAGT135300.023.3978889
GAGTACT38000.022.85881212-13
CAGAGTA125550.020.37662510-11
ATGGGGG36450.020.2486825
ATGGGGC46000.019.9267065
TAGTACA6900.019.8404434
Produced by FastQC (version 0.11.5)