##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2921073_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2773076 Sequences flagged as poor quality 0 Sequence length 125 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.12331649042435 31.0 30.0 34.0 22.0 34.0 2 29.328546711305425 31.0 30.0 34.0 22.0 34.0 3 29.516453209360293 31.0 30.0 34.0 23.0 34.0 4 32.79317443878206 37.0 35.0 37.0 24.0 37.0 5 32.967040932163414 37.0 35.0 37.0 26.0 37.0 6 32.94310469673388 37.0 35.0 37.0 25.0 37.0 7 32.86621895685513 37.0 35.0 37.0 25.0 37.0 8 32.786902342380806 37.0 35.0 37.0 24.0 37.0 9 34.13604315208094 39.0 35.0 39.0 22.0 39.0 10-11 34.122000442829545 39.0 35.0 39.0 23.0 39.0 12-13 34.04200461869779 39.0 35.0 39.0 22.5 39.0 14-15 35.180476481712006 40.0 36.0 41.0 22.0 41.0 16-17 35.153322700135156 40.0 35.0 41.0 22.5 41.0 18-19 35.1820952617238 40.0 35.0 41.0 23.0 41.0 20-21 35.22120129415855 40.0 35.0 41.0 23.0 41.0 22-23 35.14252963135522 40.0 35.0 41.0 23.0 41.0 24-25 35.110052158685875 40.0 35.0 41.0 23.5 41.0 26-27 35.018622821732976 40.0 35.0 41.0 23.0 41.0 28-29 34.8597820615086 40.0 34.5 41.0 22.5 41.0 30-31 34.72740253061943 40.0 34.0 41.0 21.5 41.0 32-33 34.57037311635166 39.5 34.0 41.0 19.5 41.0 34-35 34.420847282945005 39.0 34.0 41.0 18.5 41.0 36-37 34.27405920357033 39.0 34.0 41.0 16.5 41.0 38-39 34.11802417243523 39.0 33.5 41.0 15.5 41.0 40-41 33.97114576015947 39.0 33.0 41.0 14.0 41.0 42-43 33.771547191638454 39.0 33.0 41.0 9.0 41.0 44-45 33.5827301523651 39.0 33.0 41.0 8.5 41.0 46-47 33.36833610041701 38.0 33.0 41.0 4.5 41.0 48-49 33.103002225687284 38.0 32.5 40.5 2.0 41.0 50-51 32.90953024727776 38.0 32.0 40.0 2.0 41.0 52-53 32.71311857302143 38.0 32.0 40.0 2.0 41.0 54-55 32.47334566380438 38.0 31.5 40.0 2.0 41.0 56-57 32.21623550887173 37.0 31.0 40.0 2.0 41.0 58-59 31.929250406407903 37.0 31.0 40.0 2.0 41.0 60-61 31.617171689488494 36.0 31.0 40.0 2.0 41.0 62-63 31.322774961811362 36.0 30.5 39.5 2.0 41.0 64-65 31.000896116803148 35.0 30.0 39.0 2.0 41.0 66-67 30.648370077127346 35.0 30.0 39.0 2.0 40.5 68-69 30.279589704717793 35.0 29.5 38.0 2.0 40.0 70-71 29.91896651948955 35.0 29.0 37.0 2.0 40.0 72-73 29.567373198570827 35.0 29.0 37.0 2.0 39.0 74-75 29.51737565072144 35.0 29.0 36.5 2.0 39.0 76-77 29.562216470085925 35.0 30.0 36.0 2.0 39.0 78-79 29.39097287632939 35.0 30.0 36.0 2.0 37.5 80-81 29.174678768270326 35.0 30.0 35.0 2.0 37.0 82-83 28.926408255669877 35.0 29.5 35.0 2.0 37.0 84-85 28.67154091701778 35.0 29.0 35.0 2.0 36.0 86-87 28.521938994820193 35.0 29.0 35.0 2.0 36.0 88-89 28.337764994540358 34.0 29.0 35.0 2.0 36.0 90-91 28.180196648054363 34.0 29.0 35.0 2.0 35.0 92-93 28.05234151534253 34.0 29.0 35.0 2.0 35.0 94-95 27.91845607549162 34.0 29.0 35.0 2.0 35.0 96-97 27.797136825676613 34.0 28.5 35.0 2.0 35.0 98-99 27.67279836542525 34.0 28.0 35.0 2.0 35.0 100-101 27.515534013492598 34.0 27.0 35.0 2.0 35.0 102-103 26.245607044307476 32.5 24.5 34.5 2.0 35.0 104-105 27.139099505386795 34.0 26.5 35.0 2.0 35.0 106-107 27.243322216917242 34.0 27.0 35.0 2.0 35.0 108-109 27.145096636370585 34.0 26.5 35.0 2.0 35.0 110-111 27.012145538023482 34.0 25.0 35.0 2.0 35.0 112-113 26.859110604974404 34.0 25.0 35.0 2.0 35.0 114-115 26.678818575473592 34.0 25.0 35.0 2.0 35.0 116-117 26.493058250116476 34.0 25.0 35.0 2.0 35.0 118-119 26.232292587725688 34.0 24.0 35.0 2.0 35.0 120-121 25.964049128116216 34.0 21.5 35.0 2.0 35.0 122-123 25.600679894817162 34.0 17.5 35.0 2.0 35.0 124-125 24.316231145486096 32.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 128244.0 3 19320.0 4 13704.0 5 15265.0 6 21692.0 7 19803.0 8 16328.0 9 17153.0 10 18086.0 11 18845.0 12 19534.0 13 20208.0 14 21074.0 15 18592.0 16 13563.0 17 12149.0 18 11488.0 19 11297.0 20 11940.0 21 12903.0 22 15491.0 23 19923.0 24 26989.0 25 33230.0 26 36924.0 27 40345.0 28 46150.0 29 53708.0 30 62966.0 31 76884.0 32 94864.0 33 121665.0 34 165036.0 35 244811.0 36 439364.0 37 654446.0 38 198913.0 39 179.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.28362886407057 18.177337247143207 11.958408115574318 24.580625773211906 2 16.34785131637636 21.265948586333476 37.62033162377864 24.765868473511528 3 18.837506329237268 25.512519348609157 28.773190812563993 26.876783509589586 4 12.94718811333734 17.388661790405465 35.16047557622581 34.50367452003139 5 13.771573466643808 38.79962822259721 33.09194271740405 14.33685559335493 6 32.24885486150378 36.653494775655396 16.659228592246926 14.438421770593898 7 27.917707626323164 33.768610914552255 20.315862059006164 17.997819400118416 8 25.823656302179703 35.73049325330565 19.480120944085925 18.96572950042872 9 26.247486180763914 16.9618982629825 18.753434499889877 38.03718105636371 10-11 24.706258401641414 27.12759160406196 26.834427562148683 21.33172243214795 12-13 25.31939705718342 25.099517759801 26.55574889437745 23.025336288638137 14-15 23.151521364547236 26.286477888609117 24.136062711764374 26.425938035079273 16-17 22.957076386056233 28.678594505228734 25.014060001371707 23.350269107343326 18-19 22.994759049843168 27.72034910791954 26.64916675389552 22.635725088341772 20-21 24.31113906437382 26.786723323157663 26.194970397915895 22.70716721455262 22-23 23.973572908170997 26.655076470036303 25.800932508606937 23.57041811318576 24-25 23.479155485600835 26.771541003818193 26.085229587960505 23.664073922620467 26-27 23.281087057513858 26.821950799558607 26.251244882850163 23.64571726007737 28-29 23.326581965057834 26.99785728426798 26.276808986809296 23.398751763864894 30-31 23.636001729540567 27.0414678284521 25.89728939992305 23.425241042084284 32-33 23.221114921630477 27.226275185433447 25.96588002964192 23.58672986329416 34-35 23.512091190635644 27.129567819723693 26.066491577701008 23.29184941193965 36-37 23.657187530397366 26.91731933970104 25.94193265811098 23.48356047179061 38-39 23.396653024692696 27.145904159719365 25.89728459756177 23.560158218026174 40-41 23.42813664772261 27.09475151916126 26.09920031416423 23.37791151895191 42-43 23.398742723526393 27.077495357438863 25.970119788994673 23.55364213004007 44-45 23.307744942289883 27.20215827390056 25.86000379657572 23.630092987233834 46-47 23.298983729495877 27.139344495962238 25.79597872554634 23.765693048995548 48-49 23.28127807941662 27.067835901092614 25.840839822197076 23.810046197293694 50-51 23.138939806505636 27.280344889742786 25.72176962229824 23.858945681453335 52-53 23.291591115140527 27.030254777070063 25.635715564384338 24.042438543405073 54-55 23.14645543902632 27.006886963351217 25.684103462178008 24.162554135444456 56-57 23.220067251078333 27.0147484202891 25.627277565822116 24.137906762810456 58-59 23.11591054580569 26.666945726878478 25.840338105503523 24.37680562181231 60-61 23.285368785881825 26.74225934204272 25.81636516336687 24.15600670870858 62-63 23.133754504198446 26.672380210924707 25.94403866730407 24.249826617572776 64-65 23.119188808877023 26.531512657357574 26.002232536787595 24.34706599697781 66-67 23.182867354693986 26.718287962457065 25.865315007430496 24.23352967541846 68-69 23.377995524758195 26.461382994081134 25.891872383427167 24.268749097733505 70-71 23.15096032788489 26.300552844695158 26.224745795559883 24.323741031860074 72-73 23.279155301897227 26.2298938750944 26.212772298913496 24.278178524094876 74-75 23.12454893940868 26.348435429960116 26.27366697665286 24.25334865397835 76-77 23.074801787508413 26.059968268675632 26.46001179953354 24.405218144282415 78-79 23.08399366428735 26.126478233708976 26.491929328438278 24.297598773565397 80-81 23.191045033618842 26.036329787675605 26.45466590678834 24.317959271917214 82-83 23.222717681395448 25.90236614841974 26.420883784624692 24.45403238556012 84-85 23.138845889442422 25.874386750286828 26.43744938653822 24.549317973732528 86-87 23.050941356715818 25.792109313954604 26.560984720660763 24.59596460866882 88-89 23.211335505003532 25.73454444090618 26.497960789054204 24.55615926503608 90-91 23.24380249183438 25.72814754298648 26.52075292458212 24.507297040597024 92-93 23.178647121556917 25.745476353413395 26.474971931028 24.600904594001694 94-95 22.988470482840718 25.811222429121244 26.555337026234227 24.644970061803814 96-97 23.059445805738775 25.731963622012945 26.49310148883836 24.71548908340992 98-99 22.86333399614798 25.978352305349922 26.37355102523952 24.784762673262577 100-101 22.781417752267377 26.276232405327825 26.388971854840964 24.553377987563827 102-103 22.63648761801527 26.377595821751093 26.370974391302006 24.614942168931638 104-105 22.527133472579276 26.626600979533155 26.25519826669111 24.59106728119646 106-107 22.530935770862797 26.620382192502433 26.283093005012052 24.56558903162271 108-109 22.487314729171835 26.81462765357457 26.24296704040427 24.455090576849326 110-111 22.553913730812127 26.997379657334253 26.141130356633692 24.30757625521993 112-113 22.521707921386117 27.133146910131977 26.167985505256258 24.177159663225652 114-115 22.532896933349264 27.253625367120645 26.06391445443806 24.149563245092036 116-117 22.521492453279627 27.202299746638083 25.9933086467875 24.28289915329479 118-119 22.70520208267359 27.024864148944904 26.074596957910757 24.19533681047075 120-121 22.72846486735288 27.23152664900746 26.017608064872768 24.022400418766892 122-123 22.86481978792975 27.31563500615472 25.86169216951255 23.95785303640298 124-125 22.98941033003641 27.446446969702436 25.683505543927236 23.88063715633391 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4981.0 1 3358.0 2 1503.0 3 1147.0 4 900.5 5 745.5 6 692.5 7 704.5 8 777.0 9 832.5 10 859.5 11 909.0 12 960.5 13 1067.5 14 1233.0 15 1428.5 16 1646.5 17 1956.5 18 2342.5 19 2829.5 20 3391.5 21 4113.0 22 5091.0 23 6123.5 24 7293.0 25 8890.0 26 11296.5 27 14270.0 28 16665.0 29 19034.5 30 22336.5 31 25757.5 32 30123.5 33 34748.5 34 39138.5 35 42904.5 36 47452.5 37 53154.0 38 57288.5 39 61004.5 40 65684.5 41 70286.5 42 74517.0 43 81107.5 44 87764.5 45 93904.0 46 100523.5 47 106567.0 48 112534.0 49 117334.5 50 121275.0 51 121565.0 52 117077.0 53 114862.0 54 113347.0 55 107359.5 56 98052.5 57 87883.0 58 80484.0 59 72973.0 60 62203.5 61 52104.0 62 43336.5 63 32726.0 64 23638.5 65 17926.5 66 13341.5 67 10065.0 68 7661.0 69 5695.0 70 4450.5 71 3677.5 72 2876.5 73 2422.5 74 2130.0 75 1600.0 76 1115.5 77 756.5 78 499.0 79 324.0 80 214.0 81 152.0 82 108.5 83 84.5 84 58.5 85 41.0 86 40.0 87 31.0 88 26.5 89 27.5 90 21.0 91 16.5 92 11.5 93 12.5 94 12.5 95 9.0 96 10.0 97 13.0 98 10.5 99 14.0 100 31.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.28217762513540917 2 0.07154510009823027 3 0.07951458957489806 4 0.08560890505705578 5 0.09379476076385934 6 0.12772819785682035 7 0.11525107858565724 8 0.11543138377743704 9 0.12210267587329017 10-11 0.10039393078300053 12-13 0.08889045954744838 14-15 0.09918588599807578 16-17 0.10118727362683172 18-19 0.0932538451885199 20-21 0.09298338740085017 22-23 0.0741414948598596 24-25 0.086366186862531 26-27 0.09862693990355835 28-29 0.09110821340634011 30-31 0.08647436997759889 32-33 0.09646327760220058 34-35 0.08959364979538967 36-37 0.08952152771867775 38-39 0.07991126099681364 40-41 0.09197367832688322 42-43 0.08097506162831455 44-45 0.0778197207721678 46-47 0.06725383653387068 48-49 0.06916507156673672 50-51 0.07167131373247614 52-53 0.08438282975295304 54-55 0.08939531408443187 56-57 0.09777950550219323 58-59 0.0674341417256505 60-61 0.0847073790981567 62-63 0.08894455110498234 64-65 0.08066854280228887 66-67 0.07435786108999537 68-69 0.08207492329817141 70-71 0.0802538408611953 72-73 0.06198892493390012 74-75 0.09096396925291625 76-77 0.09475037828029235 78-79 0.07634121819957332 80-81 0.0886019712406007 82-83 0.09310960103509605 84-85 0.09705828473507397 86-87 0.084418890791309 88-89 0.06431486190785972 90-91 0.08894455110498234 92-93 0.06409849567772394 94-95 0.09141473223236579 96-97 0.08021777982283933 98-99 0.07446604420506327 100-101 0.09118033548305203 102-103 0.06388212944758817 104-105 0.07067963517768716 106-107 0.08858394072142271 108-109 0.09514704970220794 110-111 0.0882052998186851 112-113 0.07718865260093845 114-115 0.09119836600223001 116-117 0.08423858559952918 118-119 0.07639530975710727 120-121 0.10957146504459309 122-123 0.08789878099265941 124-125 0.10883221375829584 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2773076.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.60262762930572 #Duplication Level Percentage of deduplicated Percentage of total 1 69.346677479109 30.9304403293026 2 13.251375360089202 11.820923215244315 3 6.0229949370575975 8.05924201172321 4 3.3252571221344875 5.932608207610453 5 2.0223557570633584 4.510119038313983 6 1.3288584921619628 3.556234829876443 7 0.9259908693304602 2.891113815301853 8 0.650434237710255 2.320886088155346 9 0.49409008339124555 1.9833944404349106 >10 2.433281035981308 19.139566067388074 >50 0.14794185992507913 4.470796274861936 >100 0.04944225910556644 3.4634674686770763 >500 7.315351540774828E-4 0.21117819131874693 >1k 4.8769010271201716E-4 0.4743805379185588 >5k 8.128168378533618E-5 0.23564948387242124 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6500 0.23439674931375842 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3081 0.1111040591747215 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.6061038355962835E-5 2 0.0 0.0 0.0 0.0 3.6061038355962835E-5 3 0.0 0.0 0.0 0.0 3.6061038355962835E-5 4 0.0 0.0 0.0 0.0 3.6061038355962835E-5 5 0.0 0.0 0.0 0.0 3.6061038355962835E-5 6 0.0 0.0 0.0 0.0 1.0818311506788852E-4 7 0.0 0.0 0.0 0.0 1.0818311506788852E-4 8 0.0 0.0 0.0 0.0 1.0818311506788852E-4 9 0.0 0.0 0.0 0.0 1.0818311506788852E-4 10-11 0.0 0.0 0.0 3.6061038355962835E-5 1.0818311506788852E-4 12-13 0.0 0.0 0.0 7.212207671192567E-5 1.9833571095779562E-4 14-15 0.0 0.0 0.0 9.015259588990709E-5 2.5242726849173986E-4 16-17 0.0 0.0 0.0 1.0818311506788852E-4 3.2454934520366555E-4 18-19 0.0 0.0 0.0 1.0818311506788852E-4 3.2454934520366555E-4 20-21 0.0 0.0 0.0 1.6227467260183278E-4 3.6061038355962837E-4 22-23 0.0 0.0 0.0 4.507629794495354E-4 3.6061038355962837E-4 24-25 0.0 0.0 0.0 0.0010277395931449409 3.6061038355962837E-4 26-27 0.0 0.0 0.0 0.0023439674931375843 4.3273246027155407E-4 28-29 0.0 0.0 0.0 0.007031902479412753 4.507629794495355E-4 30-31 0.0 0.0 0.0 0.02226769118480705 5.228850561614612E-4 32-33 0.0 0.0 0.0 0.06166437558869645 5.769766136954054E-4 34-35 0.0 0.0 0.0 0.12576287126642038 5.769766136954054E-4 36-37 0.0 0.0 0.0 0.23372962010417314 5.769766136954054E-4 38-39 0.0 0.0 0.0 0.409491121051136 5.769766136954054E-4 40-41 0.0 0.0 0.0 0.6142817578746489 5.769766136954054E-4 42-43 0.0 0.0 0.0 0.801564760576342 5.769766136954054E-4 44-45 0.0 0.0 0.0 1.0257742665545408 5.769766136954054E-4 46-47 0.0 0.0 0.0 1.306004595618728 5.769766136954054E-4 48-49 0.0 0.0 0.0 1.594330627793829 6.130376520513683E-4 50-51 0.0 0.0 0.0 1.9007773317428012 6.130376520513683E-4 52-53 0.0 0.0 0.0 2.205817655195891 6.130376520513683E-4 54-55 0.0 0.0 0.0 2.546342040391248 6.130376520513683E-4 56-57 0.0 0.0 0.0 2.9476473057355803 6.490986904073311E-4 58-59 0.0 0.0 0.0 3.38854759119476 6.490986904073311E-4 60-61 0.0 0.0 0.0 3.86017188133322 6.490986904073311E-4 62-63 0.0 0.0 0.0 4.3263509546799295 6.490986904073311E-4 64-65 0.0 0.0 0.0 4.825165267738786 6.490986904073311E-4 66-67 0.0 0.0 0.0 5.403494170372539 6.671292095853125E-4 68-69 0.0 0.0 0.0 6.048608116041537 6.851597287632939E-4 70-71 0.0 0.0 3.6061038355962835E-5 6.767827495532037 6.851597287632939E-4 72-73 0.0 0.0 3.6061038355962835E-5 7.484612754933511 7.392512862972381E-4 74-75 0.0 0.0 3.6061038355962835E-5 8.210377212885618 7.933428438311824E-4 76-77 0.0 1.8030519177981417E-5 3.6061038355962835E-5 9.002349737259275 7.933428438311824E-4 78-79 0.0 3.6061038355962835E-5 3.6061038355962835E-5 9.880688448495462 8.294038821871452E-4 80-81 0.0 3.6061038355962835E-5 3.6061038355962835E-5 10.823378082677863 8.654649205431081E-4 82-83 0.0 3.6061038355962835E-5 3.6061038355962835E-5 11.794700181314901 9.375869972550338E-4 84-85 0.0 3.6061038355962835E-5 3.6061038355962835E-5 12.74499869459041 9.736480356109966E-4 86-87 0.0 3.6061038355962835E-5 3.6061038355962835E-5 13.72863563782601 9.736480356109966E-4 88-89 0.0 3.6061038355962835E-5 3.6061038355962835E-5 14.800531972437827 9.736480356109966E-4 90-91 0.0 3.6061038355962835E-5 3.6061038355962835E-5 15.949923478476608 9.736480356109966E-4 92-93 0.0 3.6061038355962835E-5 3.6061038355962835E-5 17.116335794619403 0.0010457701123229224 94-95 0.0 3.6061038355962835E-5 3.6061038355962835E-5 18.268594153207488 0.0010638006315009038 96-97 0.0 3.6061038355962835E-5 3.6061038355962835E-5 19.413351815817528 0.0012260753041027366 98-99 0.0 3.6061038355962835E-5 3.6061038355962835E-5 20.618872328057364 0.0012981973808146622 100-101 0.0 3.6061038355962835E-5 3.6061038355962835E-5 21.921559308147344 0.0012981973808146622 102-103 0.0 3.6061038355962835E-5 3.6061038355962835E-5 23.231242129678378 0.0013522889383486064 104-105 0.0 3.6061038355962835E-5 3.6061038355962835E-5 24.501654480439772 0.0014424415342385135 106-107 0.0 5.409155753394425E-5 3.6061038355962835E-5 25.76469234885737 0.0014424415342385135 108-109 0.0 7.212207671192567E-5 3.6061038355962835E-5 27.02709914910374 0.0014424415342385135 110-111 0.0 7.212207671192567E-5 3.6061038355962835E-5 28.342930377674467 0.0014785025725944764 112-113 0.0 7.212207671192567E-5 3.6061038355962835E-5 29.693686721892945 0.0014785025725944764 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 10325 0.0 33.048527 1 GGTATCA 7270 0.0 30.032595 1 AACGCAG 11200 0.0 29.862099 6 GTACATG 14000 0.0 29.614399 1 TCAACGC 11545 0.0 28.922878 4 TACATGG 14145 0.0 28.7392 2 CATGGGG 8540 0.0 28.15763 4 CAACGCA 12015 0.0 27.841019 5 TATCAAC 12150 0.0 27.824604 2 ATCAACG 12335 0.0 27.167011 3 ACGCAGA 12315 0.0 26.866495 7 ACATGGG 14980 0.0 26.383286 3 CGCAGAG 12705 0.0 25.85397 8 GTATAGT 695 0.0 24.891975 1 GCAGAGT 13530 0.0 23.397888 9 GAGTACT 3800 0.0 22.858812 12-13 CAGAGTA 12555 0.0 20.376625 10-11 ATGGGGG 3645 0.0 20.248682 5 ATGGGGC 4600 0.0 19.926706 5 TAGTACA 690 0.0 19.840443 4 >>END_MODULE