FastQCFastQC Report
Mon 15 Aug 2016
SRR2921073_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921073_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2773076
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103870.37456600540338597No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91920.3314730645680104No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69800.2517060477246206No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT35460.12787244201024422No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA88400.046.9064941
GTATCAA136600.041.4928551
TCAACGC173800.032.2300074
ATCAACG176650.031.8112243
TATCAAC178000.031.7044452
CAACGCA178500.031.3479885
AACGCAG180600.031.014736
ACGCAGA204100.027.3394377
GTACATG139750.026.682631
CGCAGAG209750.026.5467578
TACATGG140300.025.867222
CATGGGG84350.025.4331254
AGTACTT134600.024.90544312-13
GCAGAGT220800.024.733259
GAGTACT127800.024.5315612-13
ACATGGG145600.024.1066153
AGAGTAC204850.023.33327910-11
GTACTTT138200.022.81544314-15
CAGAGTA210850.022.27431510-11
TACTTTT155500.021.5396714-15
Produced by FastQC (version 0.11.5)