FastQCFastQC Report
Mon 15 Aug 2016
SRR2921072_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921072_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7395356
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA139400.040.9843641
GGTATCA91550.039.2151261
TCAACGC155950.035.6117634
CAACGCA160850.034.489445
GTACATG283100.034.1053471
AACGCAG165350.033.5464946
ATCAACG168350.033.236243
TACATGG289400.032.6823842
TATCAAC176500.032.071862
ACATGGG284950.031.877413
CATGGGG213600.030.2918284
ACGCAGA191950.028.8023387
CGCAGAG203350.027.0409588
GCAGAGT224150.023.7883059
ATGGGGA112050.022.6302345
AGAGTAC200600.020.23001710-11
GTATAGG16900.019.4084761
CAGAGTA220550.019.33089610-11
ATGGGGG113350.018.9048085
GTATAAG25650.018.8327221
Produced by FastQC (version 0.11.5)