##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2921072_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7395356 Sequences flagged as poor quality 0 Sequence length 125 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.50729538916044 31.0 30.0 34.0 25.0 34.0 2 29.71370803515071 31.0 30.0 34.0 25.0 34.0 3 29.900088379788613 31.0 30.0 34.0 25.0 34.0 4 33.27326013784867 37.0 35.0 37.0 27.0 37.0 5 33.35109912220588 37.0 35.0 37.0 27.0 37.0 6 33.34561040739621 37.0 35.0 37.0 27.0 37.0 7 33.23013212616134 37.0 35.0 37.0 26.0 37.0 8 33.18898982009791 37.0 35.0 37.0 26.0 37.0 9 34.625143400804504 39.0 35.0 39.0 26.0 39.0 10-11 34.56337707069139 39.0 35.0 39.0 26.5 39.0 12-13 34.482399427424454 39.0 35.0 39.0 26.0 39.0 14-15 35.65180756680273 40.0 36.0 41.0 25.5 41.0 16-17 35.60733803754681 40.0 36.0 41.0 25.0 41.0 18-19 35.62550998221046 40.0 36.0 41.0 25.0 41.0 20-21 35.66240536628663 40.0 36.0 41.0 25.5 41.0 22-23 35.58572663709496 40.0 36.0 41.0 25.0 41.0 24-25 35.55166884461005 40.0 36.0 41.0 25.0 41.0 26-27 35.49033961313019 40.0 35.5 41.0 25.0 41.0 28-29 35.36176716847708 40.0 35.0 41.0 25.0 41.0 30-31 35.2599008756306 40.0 35.0 41.0 24.5 41.0 32-33 35.14132369016448 40.0 35.0 41.0 24.0 41.0 34-35 35.03075281298155 40.0 35.0 41.0 24.0 41.0 36-37 34.91704212751895 40.0 35.0 41.0 23.5 41.0 38-39 34.781470155054066 39.0 34.5 41.0 23.0 41.0 40-41 34.64475381577303 39.0 34.0 41.0 22.0 41.0 42-43 34.458161446183254 39.0 34.0 41.0 21.0 41.0 44-45 34.274752222881496 39.0 34.0 41.0 20.0 41.0 46-47 34.09117228433628 39.0 33.0 41.0 18.5 41.0 48-49 33.85505058850446 38.0 33.0 40.5 17.0 41.0 50-51 33.676779116515824 38.0 33.0 40.0 15.0 41.0 52-53 33.48451629644334 38.0 33.0 40.0 13.5 41.0 54-55 33.2441310465649 38.0 32.5 40.0 12.0 41.0 56-57 32.99212228593187 37.5 32.0 40.0 8.5 41.0 58-59 32.70326769935078 37.0 32.0 40.0 7.5 41.0 60-61 32.39265506623346 36.5 31.5 40.0 7.0 41.0 62-63 32.10147814385136 36.0 31.0 39.5 4.0 41.0 64-65 31.76596867006808 35.5 31.0 39.0 2.0 41.0 66-67 31.420629243541487 35.0 31.0 39.0 2.0 40.5 68-69 31.059423170432904 35.0 30.0 38.0 2.0 40.0 70-71 30.69921420956611 35.0 30.0 37.5 2.0 40.0 72-73 30.351039422578168 35.0 30.0 37.0 2.0 39.0 74-75 30.306664817758605 35.0 30.0 36.5 2.0 39.0 76-77 30.355700861459542 35.0 30.5 36.0 2.0 39.0 78-79 30.180596715019533 35.0 31.0 36.0 2.0 38.0 80-81 29.972514710583233 35.0 31.0 35.5 2.0 37.0 82-83 29.73485590146032 35.0 30.0 35.0 2.0 37.0 84-85 29.485941920848706 35.0 30.0 35.0 2.0 36.0 86-87 29.343161978950032 35.0 30.0 35.0 2.0 36.0 88-89 29.17080976223457 35.0 30.0 35.0 2.0 36.0 90-91 29.029545771697805 34.5 30.0 35.0 2.0 35.5 92-93 28.923925298525184 34.0 30.0 35.0 2.0 35.0 94-95 28.809837011227046 34.0 30.0 35.0 2.0 35.0 96-97 28.71078214490283 34.0 29.0 35.0 2.0 35.0 98-99 28.607399630254445 34.0 29.0 35.0 2.0 35.0 100-101 28.479495713255723 34.0 29.0 35.0 2.0 35.0 102-103 27.19310571391019 32.5 26.0 34.5 2.0 35.0 104-105 28.141730769958876 34.0 29.0 35.0 2.0 35.0 106-107 28.284010871146705 34.0 29.0 35.0 2.0 35.0 108-109 28.219898136073503 34.0 29.0 35.0 2.0 35.0 110-111 28.118164088381953 34.0 29.0 35.0 2.0 35.0 112-113 28.00500313980828 34.0 29.0 35.0 2.0 35.0 114-115 27.87263885606048 34.0 29.0 35.0 2.0 35.0 116-117 27.739077875358536 34.0 29.0 35.0 2.0 35.0 118-119 27.53906444801305 34.0 28.0 35.0 2.0 35.0 120-121 27.339406649253938 34.0 27.5 35.0 2.0 35.0 122-123 27.047747938030298 34.0 27.0 35.0 2.0 35.0 124-125 25.817755088463624 33.0 22.5 34.5 2.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 300950.0 3 45948.0 4 29674.0 5 26700.0 6 31623.0 7 34166.0 8 35164.0 9 38419.0 10 41564.0 11 44350.0 12 45147.0 13 44990.0 14 44702.0 15 40117.0 16 33213.0 17 30149.0 18 28823.0 19 28609.0 20 29526.0 21 31839.0 22 37919.0 23 50079.0 24 67264.0 25 85018.0 26 95021.0 27 106075.0 28 123827.0 29 144412.0 30 168559.0 31 204337.0 32 253775.0 33 325896.0 34 443446.0 35 668148.0 36 1224808.0 37 1853583.0 38 557108.0 39 408.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.48374978019724 17.443072610088137 11.796246467056157 25.276931142658466 2 16.3144319121129 19.81406583765562 38.11770751740088 25.753794732830592 3 18.883151974612034 24.209488064749486 29.071193638718924 27.83616632191956 4 12.990768398820544 15.995474124194178 35.72964049438197 35.28411698260331 5 14.066840869868368 37.689293053308994 33.92423297539935 14.31963310142329 6 33.53096211289236 35.72750080686911 16.758536025351937 13.983001054886593 7 28.907232253180844 32.09461252150974 20.865717512851695 18.132437712457726 8 26.92879043229304 34.73788300270056 19.54377415446676 18.78955241053964 9 26.729139574721785 15.54272831216202 18.69875544241806 39.029376670698134 10-11 25.540179668399755 25.754230135991413 27.328492485799956 21.37709770980888 12-13 25.903016015942793 23.340203975589404 27.199122607638465 23.557657400829335 14-15 23.293568208851866 24.71697437757913 24.27455767634866 27.714899737220343 16-17 23.738175262430758 27.40872403988562 25.124237357931428 23.7288633397522 18-19 23.90973432320265 26.648604941871422 26.327665004967233 23.113995729958695 20-21 24.673229947050938 25.877285224986156 25.988410050199022 23.461074777763884 22-23 24.454767105172266 25.58660856009099 25.878413390898654 24.08021094383809 24-25 24.078422032167122 25.862990488711667 25.98908365420497 24.06950382491624 26-27 23.85008212381921 25.998321589580943 25.98226970056392 24.169326586035925 28-29 23.96900798484233 26.205616456895385 25.928522127486804 23.89685343077548 30-31 24.118171301238014 26.05307797026663 25.741468374623587 24.087282353871768 32-33 23.804875163703564 26.103583474657675 25.70378575552126 24.3877556061175 34-35 24.17292922731302 26.067389347895226 25.82648207168619 23.933199353105568 36-37 24.36559506783406 25.780679066010865 25.714555605959394 24.13917026019568 38-39 24.050825356899573 26.047261300365065 25.75762927046044 24.14428407227492 40-41 24.16192130567108 25.91340290511978 25.944489409577688 23.98018637963145 42-43 24.064351599909525 25.976342959360853 25.73882665565691 24.220478785072714 44-45 24.011571789655836 25.984537839505894 25.865438364956294 24.13845200588197 46-47 24.131372291942093 25.97473306897647 25.798073461117095 24.095821177964343 48-49 24.022813478307874 26.153734369906985 25.523564902644985 24.299887249140163 50-51 23.971537774475006 26.243848968401963 25.65040558549594 24.13420767162709 52-53 24.1589029865728 26.00527052209456 25.628436379526036 24.2073901118066 54-55 23.940591447889766 26.01667391710787 25.741806747694323 24.300927887308045 56-57 23.900943852049995 26.145749336347684 25.65504452906062 24.298262282541703 58-59 23.890479048288416 25.798447076241125 25.708449669340883 24.60262420612958 60-61 23.79988569002754 25.97179546566834 25.900634226154168 24.327684618149952 62-63 23.727142426203933 25.937955776154354 25.93089139378234 24.40401040385937 64-65 23.663017616458312 25.89533781088788 25.86074293394733 24.58090163870648 66-67 23.669186671194247 25.941601954842074 25.807702823085698 24.581508550877984 68-69 23.824869130688924 25.741227390295396 25.943379853592546 24.49052362542313 70-71 23.587549921844985 25.680556215248558 26.04567430164899 24.68621956125747 72-73 23.653323319503432 25.722991731351907 25.99497382768478 24.62871112145988 74-75 23.556506380513344 25.840405763107178 26.0022920126418 24.60079584373768 76-77 23.582225436674793 25.581785314117173 26.129732484793642 24.706256764414388 78-79 23.461062773169512 25.70129632887241 26.205132542184817 24.632508355773265 80-81 23.451219616995044 25.67357702102879 26.215731269806287 24.659472092169878 82-83 23.480965324371468 25.593624034637735 26.244191315231312 24.68121932575949 84-85 23.295093159543523 25.63227162359039 26.224148768886202 24.84848644797989 86-87 23.305833975911245 25.576520270773173 26.317104282749078 24.800541470566504 88-89 23.29714364729689 25.472374104379348 26.18335037215714 25.047131876166628 90-91 23.140229900924002 25.523598444302664 26.32178077096432 25.014390883809018 92-93 23.22454348013156 25.454368578942045 26.440119194968524 24.880968745957876 94-95 23.093233700572824 25.238712887071895 26.567085785062716 25.100967627292565 96-97 23.172979361602682 25.04075878458712 26.56022787029396 25.22603398351624 98-99 23.035957910689607 25.25613689681827 26.403522214272545 25.304382978219575 100-101 22.994238299514773 25.270728466568087 26.560601520772785 25.174431713144347 102-103 22.839067533164766 25.13694402471338 26.702792702885247 25.3211957392366 104-105 22.722966548125527 25.29523373960707 26.528438203284804 25.453361508982596 106-107 22.713184840784553 25.28270842527337 26.510607799600695 25.49349893434138 108-109 22.65058114694117 25.279225402673706 26.60135990048888 25.468833549896242 110-111 22.669175939079164 25.404238210093848 26.607618514808994 25.318967336017995 112-113 22.67368416067301 25.417252331750472 26.639657569025598 25.26940593855092 114-115 22.522116339691227 25.59521791607399 26.544838262138875 25.33782748209591 116-117 22.433746369049995 25.479508687553242 26.6205522765456 25.46619266685116 118-119 22.580042785650438 25.412246081562284 26.547265396944518 25.460445735842757 120-121 22.407233787362596 25.50919671004691 26.716219855299617 25.367349647290872 122-123 22.528384596823493 25.58027430718469 26.57803363698382 25.313307459007994 124-125 22.503962555039248 25.65896173613376 26.450938086684868 25.38613762214212 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2973.0 1 2273.0 2 1347.0 3 1006.0 4 813.5 5 697.0 6 674.5 7 728.0 8 799.0 9 885.5 10 929.5 11 957.5 12 1051.0 13 1223.5 14 1384.5 15 1569.5 16 1851.0 17 2187.5 18 2613.0 19 3073.0 20 3688.5 21 4662.5 22 5822.0 23 7529.5 24 9734.5 25 12981.0 26 18248.0 27 24761.5 28 31326.0 29 38946.0 30 49399.0 31 62003.0 32 76705.5 33 87097.5 34 94727.5 35 105794.0 36 120480.0 37 136242.5 38 149801.0 39 162647.5 40 176302.0 41 193037.0 42 207940.0 43 224820.5 44 242931.0 45 260712.5 46 280534.0 47 292021.5 48 300529.0 49 309037.0 50 312300.0 51 314512.5 52 314243.0 53 312632.0 54 308848.5 55 293875.0 56 271254.0 57 246651.5 58 225604.0 59 203917.5 60 177278.5 61 152034.5 62 126793.5 63 98733.0 64 74087.0 65 57211.0 66 44007.5 67 33858.0 68 25766.0 69 19586.0 70 15585.5 71 11944.5 72 8867.0 73 7237.0 74 5905.5 75 4516.5 76 3223.0 77 2066.0 78 1371.0 79 975.5 80 664.0 81 453.0 82 300.0 83 216.5 84 171.0 85 128.5 86 102.5 87 88.0 88 68.5 89 48.5 90 39.0 91 31.0 92 23.5 93 16.5 94 13.5 95 13.0 96 12.0 97 9.5 98 10.5 99 15.0 100 22.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.26266483993468337 2 0.06467572352162627 3 0.072991753202956 4 0.07906313097030082 5 0.08497224474386358 6 0.1187231554505287 7 0.1082300838526232 8 0.10825712785158687 9 0.11458542360908655 10-11 0.09345729941871629 12-13 0.08366737179386632 14-15 0.09257836945239688 16-17 0.09436327338399937 18-19 0.08700730566588004 20-21 0.08610809270033788 22-23 0.06953688233534668 24-25 0.08084127390216238 26-27 0.09218623146742361 28-29 0.08594582870655584 30-31 0.08088183990060789 32-33 0.08950887557001988 34-35 0.0839175287842803 36-37 0.08313325281433374 38-39 0.07385716116979359 40-41 0.08515479173686838 42-43 0.07501329212549065 44-45 0.07186942724596354 46-47 0.0620524556221499 48-49 0.06389144755167972 50-51 0.06664993544597447 52-53 0.07796108801253111 54-55 0.08316705781303835 56-57 0.09057035252934409 58-59 0.062032172622927144 60-61 0.07842083599491356 62-63 0.08349834680034335 64-65 0.07482398413274492 66-67 0.06884726036177298 68-69 0.07621675007937413 70-71 0.07511470712160442 72-73 0.05739412680065706 74-75 0.0840662707785805 76-77 0.08796060662934956 78-79 0.07107162927653517 80-81 0.0828966178234016 82-83 0.08681123667339341 84-85 0.0900632775487752 86-87 0.07910369696874633 88-89 0.05938862172422801 90-91 0.08288309582391977 92-93 0.060037677699356196 94-95 0.08591878470759218 96-97 0.07488483313041319 98-99 0.06921235434778258 100-101 0.08407979277806234 102-103 0.059226357730445975 104-105 0.0650881445058223 106-107 0.08152413487599515 108-109 0.0869870226666573 110-111 0.0806316829101939 112-113 0.07103782427783058 114-115 0.0837349817912755 116-117 0.07790023901486284 118-119 0.07114600027368527 120-121 0.10100257512958133 122-123 0.0817945748656319 124-125 0.10068480814175815 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 7395356.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.806562616308035 #Duplication Level Percentage of deduplicated Percentage of total 1 69.81846737595787 29.18870128127642 2 12.969826619257258 10.844477373612746 3 5.762962469934632 7.227889540642661 4 3.1891277231284785 5.333058713934984 5 1.9587762224387715 4.094485039736091 6 1.2636372364651582 3.1696997550347454 7 0.8645230555685591 2.529991607910824 8 0.665553873331724 2.2259615743975254 9 0.48526379308958395 1.8258490036104154 >10 2.7590291512278386 20.806160156587353 >50 0.17839770152907858 5.09943600385896 >100 0.0803573281196642 5.880977941858577 >500 0.002783110364580879 0.7775179751782545 >1k 0.001294339586901244 0.9957940323604642 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 1.352199948183698E-5 0.0 0.0 2.704399896367396E-5 5 0.0 1.352199948183698E-5 0.0 0.0 2.704399896367396E-5 6 0.0 1.352199948183698E-5 0.0 0.0 5.408799792734792E-5 7 0.0 1.352199948183698E-5 0.0 0.0 5.408799792734792E-5 8 0.0 2.704399896367396E-5 0.0 0.0 6.76099974091849E-5 9 0.0 2.704399896367396E-5 0.0 0.0 9.465399637285886E-5 10-11 0.0 2.704399896367396E-5 0.0 6.76099974091849E-6 9.465399637285886E-5 12-13 0.0 2.704399896367396E-5 0.0 2.0282999222755468E-5 1.2169799533653282E-4 14-15 0.0 4.0565998445510936E-5 0.0 2.704399896367396E-5 1.7578599326388073E-4 16-17 0.0 4.0565998445510936E-5 0.0 4.0565998445510936E-5 2.1635199170939168E-4 18-19 0.0 4.0565998445510936E-5 0.0 5.408799792734792E-5 2.974839886004136E-4 20-21 0.0 4.0565998445510936E-5 0.0 7.437099715010338E-5 2.974839886004136E-4 22-23 0.0 4.0565998445510936E-5 0.0 1.2169799533653282E-4 3.245279875640875E-4 24-25 0.0 4.0565998445510936E-5 0.0 2.974839886004135E-4 3.5157198652776146E-4 26-27 0.0 4.0565998445510936E-5 0.0 7.775149702056263E-4 3.6509398600959844E-4 28-29 0.0 4.0565998445510936E-5 0.0 0.0030154058844496464 3.786159854914354E-4 30-31 0.0 4.0565998445510936E-5 0.0 0.01267687451422217 3.786159854914354E-4 32-33 0.0 4.0565998445510936E-5 0.0 0.03433911768412501 4.056599844551094E-4 34-35 0.0 4.0565998445510936E-5 0.0 0.06550732648975925 4.191819839369464E-4 36-37 0.0 4.0565998445510936E-5 0.0 0.11983195940803931 4.2594298367786487E-4 38-39 0.0 4.0565998445510936E-5 0.0 0.21249146085732723 4.3270398341878336E-4 40-41 0.0 4.0565998445510936E-5 0.0 0.31617139188431226 4.4622598290062034E-4 42-43 0.0 4.0565998445510936E-5 0.0 0.4226774208030012 4.4622598290062034E-4 44-45 0.0 4.0565998445510936E-5 0.0 0.5522519808377041 4.597479823824573E-4 46-47 0.0 4.0565998445510936E-5 0.0 0.7100401927912598 4.732699818642943E-4 48-49 0.0 4.0565998445510936E-5 0.0 0.880315971266292 4.867919813461313E-4 50-51 0.0 4.0565998445510936E-5 0.0 1.0643436232143524 5.003139808279683E-4 52-53 0.0 4.0565998445510936E-5 0.0 1.2498113681072285 5.341189795325607E-4 54-55 0.0 4.0565998445510936E-5 0.0 1.4601325480477207 5.814459777189902E-4 56-57 0.0 4.0565998445510936E-5 0.0 1.7094038474956446 5.949679772008272E-4 58-59 0.0 4.0565998445510936E-5 0.0 1.9840559399709763 6.287729759054195E-4 60-61 0.0 4.0565998445510936E-5 0.0 2.282385053539005 6.35533975646338E-4 62-63 0.0 4.0565998445510936E-5 0.0 2.5877523678373295 6.35533975646338E-4 64-65 0.0 4.0565998445510936E-5 0.0 2.914612900312034 6.35533975646338E-4 66-67 0.0 4.0565998445510936E-5 0.0 3.292309389838704 6.49055975128175E-4 68-69 0.0 4.0565998445510936E-5 0.0 3.719300328476411 6.693389743509305E-4 70-71 0.0 4.0565998445510936E-5 0.0 4.183848620674921 6.963829733146044E-4 72-73 0.0 4.0565998445510936E-5 0.0 4.661885107356563 7.775149702056263E-4 74-75 0.0 5.408799792734792E-5 0.0 5.1494546036729 7.842759699465448E-4 76-77 0.0 5.408799792734792E-5 0.0 5.693464925826424 7.842759699465448E-4 78-79 0.0 5.408799792734792E-5 0.0 6.304490277411933 7.977979694283818E-4 80-81 0.0 5.408799792734792E-5 0.0 6.950280689665244 8.113199689102188E-4 82-83 0.0 5.408799792734792E-5 0.0 7.621857554930418 8.721689665784852E-4 84-85 0.0 5.408799792734792E-5 0.0 8.299262401972264 8.789299663194037E-4 86-87 0.0 5.408799792734792E-5 0.0 9.014515325563773 9.059739652830777E-4 88-89 0.0 5.408799792734792E-5 0.0 9.779346660255436 9.465399637285886E-4 90-91 0.0 6.084899766826641E-5 6.76099974091849E-6 10.610550729403695 9.465399637285886E-4 92-93 0.0 6.76099974091849E-5 1.352199948183698E-5 11.455594565021617 9.735839626922626E-4 94-95 0.0 8.113199689102187E-5 1.352199948183698E-5 12.296236989808198 0.0010817599585469584 96-97 0.0 8.113199689102187E-5 1.352199948183698E-5 13.151097797049932 0.001102042957769714 98-99 0.0 8.113199689102187E-5 1.352199948183698E-5 14.052771766497784 0.0011088039575106324 100-101 0.0 8.113199689102187E-5 1.352199948183698E-5 15.016376493572452 0.0011088039575106324 102-103 0.0 8.789299663194036E-5 1.352199948183698E-5 16.01179848542788 0.0011628919554379803 104-105 0.0 9.465399637285886E-5 1.352199948183698E-5 16.99373904379992 0.0011764139549198173 106-107 0.0 1.0141499611377735E-4 1.352199948183698E-5 17.959906189776397 0.0011764139549198173 108-109 6.76099974091849E-6 1.0817599585469584E-4 1.352199948183698E-5 18.952928026723797 0.0011966969541425728 110-111 1.352199948183698E-5 1.2845899507745131E-4 1.352199948183698E-5 19.99305374886618 0.0012237409531062468 112-113 1.352199948183698E-5 1.5550299404112525E-4 1.352199948183698E-5 21.06031812396861 0.0012305019528471653 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 13940 0.0 40.984364 1 GGTATCA 9155 0.0 39.215126 1 TCAACGC 15595 0.0 35.611763 4 CAACGCA 16085 0.0 34.48944 5 GTACATG 28310 0.0 34.105347 1 AACGCAG 16535 0.0 33.546494 6 ATCAACG 16835 0.0 33.23624 3 TACATGG 28940 0.0 32.682384 2 TATCAAC 17650 0.0 32.07186 2 ACATGGG 28495 0.0 31.87741 3 CATGGGG 21360 0.0 30.291828 4 ACGCAGA 19195 0.0 28.802338 7 CGCAGAG 20335 0.0 27.040958 8 GCAGAGT 22415 0.0 23.788305 9 ATGGGGA 11205 0.0 22.630234 5 AGAGTAC 20060 0.0 20.230017 10-11 GTATAGG 1690 0.0 19.408476 1 CAGAGTA 22055 0.0 19.330896 10-11 ATGGGGG 11335 0.0 18.904808 5 GTATAAG 2565 0.0 18.832722 1 >>END_MODULE