FastQCFastQC Report
Mon 15 Aug 2016
SRR2921072_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921072_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7395356
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107620.14552375842352958No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91900.12426717523808185No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA111650.054.810471
GTATCAA187550.048.403021
TCAACGC234700.038.062864
CAACGCA240950.037.026585
ATCAACG242350.036.9105533
TATCAAC247650.036.26552
AACGCAG251300.035.5772866
GTACATG284050.032.8200381
TACATGG291500.031.0759052
ACGCAGA292200.030.477237
ACATGGG289600.030.1475433
CGCAGAG304750.029.0860848
CATGGGG213000.028.1189674
GCAGAGT327800.026.350139
AGAGTAC303400.023.8417810-11
GAGTACT184200.023.09202812-13
CAGAGTA323750.022.40746510-11
GTACTTT193850.021.92825714-15
AGTACTT196650.021.7813212-13
ATGGGGA106400.019.6040215
Produced by FastQC (version 0.11.5)