##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921072_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7395356
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.520527206533398	33.0	31.0	34.0	28.0	34.0
2	31.582635778453398	33.0	31.0	34.0	28.0	34.0
3	31.667185595933447	34.0	31.0	34.0	28.0	34.0
4	35.168678559896236	37.0	35.0	37.0	32.0	37.0
5	34.95019631238848	37.0	35.0	37.0	32.0	37.0
6	34.73350965119191	37.0	35.0	37.0	32.0	37.0
7	34.68610692980838	37.0	35.0	37.0	32.0	37.0
8	34.68086079966941	37.0	35.0	37.0	32.0	37.0
9	36.260636404792415	39.0	37.0	39.0	32.0	39.0
10-11	36.232412611373945	39.0	37.0	39.0	32.0	39.0
12-13	36.18213281416067	39.0	37.0	39.0	31.5	39.0
14-15	37.48107717870512	40.0	38.0	41.0	32.0	41.0
16-17	37.480697413349674	40.0	38.0	41.0	31.5	41.0
18-19	37.4967012406164	40.0	38.0	41.0	32.0	41.0
20-21	37.50547701828012	40.0	38.0	41.0	31.5	41.0
22-23	37.47169358716471	40.0	38.0	41.0	31.0	41.0
24-25	37.43575610153182	40.0	38.0	41.0	31.0	41.0
26-27	37.33551758698296	40.0	37.5	41.0	31.0	41.0
28-29	37.22294518343674	40.0	37.0	41.0	31.0	41.0
30-31	37.106645711173336	40.0	37.0	41.0	30.0	41.0
32-33	37.00553475721791	40.0	37.0	41.0	30.0	41.0
34-35	36.88276798304233	40.0	37.0	41.0	30.0	41.0
36-37	36.72290001995847	40.0	36.0	41.0	30.0	41.0
38-39	36.590899275166734	40.0	36.0	41.0	30.0	41.0
40-41	36.46503298826994	40.0	36.0	41.0	30.0	41.0
42-43	36.32005849346536	40.0	36.0	41.0	29.0	41.0
44-45	36.16094039286276	40.0	35.0	41.0	28.5	41.0
46-47	35.9246450745576	40.0	35.0	41.0	28.0	41.0
48-49	35.69899900694436	40.0	35.0	41.0	27.0	41.0
50-51	35.513525447591704	39.0	35.0	41.0	26.5	41.0
52-53	35.30278407151731	39.0	35.0	41.0	26.0	41.0
54-55	35.092084816471306	39.0	35.0	41.0	26.0	41.0
56-57	34.84402022025715	38.5	34.5	41.0	26.0	41.0
58-59	34.5872574626563	38.0	34.0	40.5	25.0	41.0
60-61	34.30069147448751	37.5	34.0	40.0	24.5	41.0
62-63	34.008262685393376	37.0	34.0	40.0	24.0	41.0
64-65	33.6916911775444	36.5	34.0	40.0	23.0	41.0
66-67	33.36042382543856	36.0	33.0	39.0	23.0	41.0
68-69	33.01047393796864	35.5	33.0	39.0	22.0	41.0
70-71	32.63437838557062	35.0	33.0	38.5	22.0	40.0
72-73	32.26403326628225	35.0	32.5	37.0	21.0	39.5
74-75	31.946617985665597	35.0	32.0	37.0	20.0	39.0
76-77	31.65629511277077	35.0	32.0	36.0	20.0	39.0
78-79	31.365247595923712	35.0	32.0	36.0	20.0	37.5
80-81	31.334932354845392	35.0	32.0	36.0	20.0	37.0
82-83	31.14890973470378	35.0	32.0	35.0	19.5	37.0
84-85	30.964751392630728	35.0	32.0	35.0	19.0	36.5
86-87	30.80628214517327	35.0	32.0	35.0	18.0	36.0
88-89	30.643255037350468	35.0	32.0	35.0	18.0	36.0
90-91	30.503690897368564	35.0	32.0	35.0	17.0	35.5
92-93	30.410106288324727	35.0	32.0	35.0	15.5	35.0
94-95	30.31157080200061	35.0	32.0	35.0	13.5	35.0
96-97	30.247437040218212	35.0	32.0	35.0	10.5	35.0
98-99	30.159212484158978	35.0	31.0	35.0	8.5	35.0
100-101	30.07504101222443	35.0	31.0	35.0	7.0	35.0
102-103	28.737081622575033	33.0	28.5	34.5	7.0	35.0
104-105	29.764105068099497	34.0	31.0	35.0	6.0	35.0
106-107	29.937058608131913	35.0	31.0	35.0	2.0	35.0
108-109	29.93999403138943	35.0	31.0	35.0	2.0	35.0
110-111	29.852579645929147	35.0	31.0	35.0	2.0	35.0
112-113	29.748434017240008	35.0	31.0	35.0	2.0	35.0
114-115	29.6660543454568	35.0	31.0	35.0	2.0	35.0
116-117	29.565975458111822	35.0	31.0	35.0	2.0	35.0
118-119	29.420766356616234	35.0	31.0	35.0	2.0	35.0
120-121	29.561374665398123	35.0	31.5	35.0	2.0	35.0
122-123	29.479991226926735	35.0	32.0	35.0	2.0	35.0
124-125	28.8136038346227	34.5	30.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	97949.0
3	39744.0
4	17617.0
5	15848.0
6	17756.0
7	21294.0
8	24398.0
9	27258.0
10	31287.0
11	35445.0
12	38385.0
13	43659.0
14	54558.0
15	48879.0
16	31212.0
17	27379.0
18	25792.0
19	24849.0
20	25424.0
21	26029.0
22	27750.0
23	32543.0
24	42388.0
25	56031.0
26	70994.0
27	84089.0
28	101219.0
29	122395.0
30	147102.0
31	181634.0
32	229254.0
33	298245.0
34	411124.0
35	639664.0
36	1260680.0
37	2216271.0
38	798091.0
39	1120.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.1174698203746	17.97612171924163	12.330363387809793	25.576045072573965
2	16.515199319272593	20.134741724292144	37.39859783894473	25.951461117490528
3	18.92893323864328	24.070497755618526	29.635287334375786	27.365281671362407
4	13.362157036761376	16.06122510520859	35.556034553285556	35.02058330474448
5	13.946168729444993	37.31517925929537	34.25266766589896	14.485984345360677
6	33.614016200663826	35.782512251713115	16.461915531005896	14.141556016617162
7	28.832773733909967	31.80963351229967	21.05502014706676	18.302572606723604
8	27.355938492862546	34.17372875052707	19.47851785898299	18.991814897627393
9	27.053356406100903	14.567772378024108	18.883070510060346	39.49580070581464
10-11	25.880260822829644	25.087669252451818	27.501840268295464	21.530229656423074
12-13	26.368737302590482	22.675357285478267	27.575585380752234	23.380320031179018
14-15	23.57757710188806	24.017893680305242	24.876652666295765	27.527876551510932
16-17	23.642698571023917	26.89591195332166	25.638147664220135	23.823241811434283
18-19	23.748187729576795	25.885557827655788	27.36716328286025	22.999091159907167
20-21	24.43655423741536	25.127129561316963	27.16535465019168	23.270961551076
22-23	24.188287777868094	24.91950875215945	27.061336731559642	23.830866738412812
24-25	23.86523432296008	25.26855768243697	27.116917554938603	23.749290439664346
26-27	23.61487380672902	25.392728044375218	27.160952666517623	23.83144548237814
28-29	23.723582507079808	25.550651975130368	27.113842365284867	23.611923152504957
30-31	23.89880693151528	25.38119574102737	26.97000611871608	23.74999120874127
32-33	23.593115353474246	25.446753622465117	26.90006088244859	24.060070141612048
34-35	23.863907705089677	25.466441875347122	27.003137282004612	23.66651313755859
36-37	24.149139662102513	25.174068585477443	26.822744682321538	23.854047070098506
38-39	23.89586885311528	25.404072633703766	26.83073123353535	23.869327279645603
40-41	23.96379185353899	25.268615999697037	26.994849627517702	23.772742519246272
42-43	23.963136663366402	25.295683216748838	26.739795455696598	24.001384664188166
44-45	23.903622436447485	25.34671462749295	26.7803184415635	23.969344494496063
46-47	24.106360089579717	25.3423416185645	26.566380447622674	23.98491784423311
48-49	24.00215279226001	25.607110949541244	26.211922774291672	24.178813483907074
50-51	23.975200647139555	25.752636814426726	26.212276028579247	24.05988650985447
52-53	24.15921491901628	25.577538195158446	26.197096964423043	24.06614992140224
54-55	23.90571514005567	25.583246902592272	26.274447141916053	24.236590815436003
56-57	23.87448810862179	25.718439828242783	26.093318570792373	24.31375349234305
58-59	23.772374534742546	25.42100115255711	26.262540271035185	24.54408404166516
60-61	23.73753711833386	25.580859577912967	26.437930139566422	24.243673164186745
62-63	23.592119745123526	25.603505244606882	26.44082746070661	24.363547549562977
64-65	23.498738535762833	25.523614000531946	26.41035502304327	24.56729244066195
66-67	23.508498665750253	25.621747128059706	26.326686225654893	24.54306798053514
68-69	23.694702425260097	25.458002352121454	26.352834987968908	24.49446023464954
70-71	23.440051168079947	25.427953796165152	26.429783468890694	24.702211566864204
72-73	23.573747844002053	25.511325887340586	26.241504502437873	24.673421766219487
74-75	23.510350151360075	25.6360991078519	26.1745282304395	24.679022510348528
76-77	23.539010334369852	25.435595504700238	26.196293187751568	24.82910097317835
78-79	23.40354950172592	25.64158548386366	26.165287337186705	24.78957767722372
80-81	23.375448730861425	25.691291830297185	26.078399432321525	24.854860006519864
82-83	23.366069343848743	25.60013183030378	26.125640852085198	24.90815797376228
84-85	23.15267401810648	25.656776940517815	26.031288844723484	25.159260196652223
86-87	23.165588751365	25.6368873547519	26.09956488959961	25.09795900428349
88-89	23.12008225039549	25.6009630502108	25.926828308385424	25.352126391008284
90-91	22.94122949323701	25.68168341667257	25.967056642480742	25.41003044760968
92-93	23.039915578712293	25.614004813273823	26.04864786170672	25.297431746307158
94-95	22.89682481188681	25.467350100755727	26.114199018015405	25.521626069342062
96-97	22.98371600970863	25.300757373352585	26.05479019829683	25.660736418641957
98-99	22.85163935451652	25.519217121196704	25.84793668643835	25.78120683784843
100-101	22.766309202562024	25.530378633061428	25.93219861016463	25.771113554211915
102-103	22.684714713585656	25.36538024078188	25.975983753484883	25.97392129214758
104-105	22.569779199494544	25.585769591276115	25.750648826567517	26.093802382661824
106-107	22.570363226049583	25.637660872035458	25.67431164340233	26.11766425851263
108-109	22.447349495706796	25.723564939566224	25.66279849394959	26.16628707077739
110-111	22.452355124908987	25.846097499899745	25.60265171963675	26.098895655554514
112-113	22.4008072231378	25.949435222398847	25.557028323946295	26.092729230517058
114-115	22.273017383358976	26.171028537438122	25.374465084895025	26.181488994307877
116-117	22.181072159870922	26.139797261940124	25.397027066518874	26.28210351167008
118-119	22.249145572232827	26.183749350816104	25.24655127077093	26.32055380618014
120-121	22.021614224496393	26.351369076676136	25.38079790348513	26.24621879534234
122-123	22.0485260784467	26.44469247876345	25.168910650038935	26.337870792750916
124-125	21.93093793538858	26.605744014113764	25.102464953068036	26.360853097429615
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	69.0
1	88.0
2	288.5
3	520.5
4	415.0
5	318.5
6	557.5
7	1188.0
8	1877.5
9	2123.5
10	1799.5
11	1445.5
12	1565.0
13	1849.5
14	2134.0
15	2463.5
16	2863.5
17	3294.0
18	3720.5
19	4215.5
20	4799.5
21	5635.0
22	6821.0
23	8507.0
24	10539.0
25	13544.0
26	18783.5
27	25410.0
28	32116.5
29	40490.0
30	50983.0
31	63059.5
32	78427.5
33	88488.5
34	94552.5
35	105140.0
36	119075.5
37	134551.0
38	148988.0
39	161360.0
40	174346.0
41	190443.5
42	204675.5
43	221351.0
44	240428.0
45	259160.5
46	279030.0
47	290935.5
48	300056.5
49	308738.5
50	313194.0
51	315784.5
52	314780.0
53	314673.5
54	312220.5
55	297203.5
56	273504.0
57	248720.5
58	227852.5
59	205597.5
60	178728.0
61	152862.0
62	127040.0
63	98302.5
64	72746.0
65	55468.5
66	42071.5
67	31960.0
68	24202.5
69	18232.5
70	14296.0
71	10942.0
72	8223.0
73	6749.5
74	5362.0
75	3992.0
76	2762.5
77	1699.0
78	1016.5
79	619.0
80	398.5
81	242.5
82	152.5
83	118.0
84	87.5
85	53.5
86	43.5
87	29.5
88	23.5
89	20.0
90	18.0
91	17.0
92	15.5
93	13.5
94	15.5
95	28.5
96	32.5
97	24.5
98	13.5
99	4.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.14632155639295796
2	0.012345585526917164
3	0.0
4	0.0038537698523235394
5	0.130974086981073
6	0.069841127323688
7	0.006531125749727261
8	0.00949244363624956
9	0.010547159595832844
10-11	0.005063988805947949
12-13	0.00864055766889383
14-15	0.010682379590651214
16-17	0.013596370478987084
18-19	0.017794951318097465
20-21	0.020566961211874048
22-23	0.02291978912171368
24-25	0.022892745122750007
26-27	0.02026947722327363
28-29	0.019458157254363416
30-31	0.022284255146067345
32-33	0.02162843817119825
34-35	0.020763030204360683
36-37	0.021297149183893242
38-39	0.017517750328719807
40-41	0.023203751110832255
42-43	0.020357370219905573
44-45	0.02839619891185766
46-47	0.02323079510979593
48-49	0.01674023535851418
50-51	0.013109578497640953
52-53	0.014353602449969954
54-55	0.01607765738390417
56-57	0.013048729499972687
58-59	0.012555176518885635
60-61	0.014860677430538842
62-63	0.01285266050748605
64-65	0.02387309008518319
66-67	0.017754385319651955
68-69	0.015955959388567635
70-71	0.022771047127413475
72-73	0.027422614949165394
74-75	0.014441495446601893
76-77	0.007646690706978812
78-79	0.011919642543239298
80-81	0.01392089846655117
82-83	0.013542282481059735
84-85	0.012595742517331147
86-87	0.009208481647130983
88-89	0.01824117730099809
90-91	0.01594919838882672
92-93	0.013691024475359944
94-95	0.009992757617077528
96-97	0.017774668318874712
98-99	0.018437246293484724
100-101	0.023616172095028285
102-103	0.01728111533778766
104-105	0.011534265558006943
106-107	0.016787562356700612
108-109	0.008789299663194037
110-111	0.020452024216278432
112-113	0.015117595420693743
114-115	0.011378762563965819
116-117	0.010013040616300284
118-119	0.006686628743768386
120-121	0.0030559718828951574
122-123	0.004354083833151508
124-125	3.04244988341332E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	7395356.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.007038891557386
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.5481796232444	24.912922122114395
2	14.673858969157177	11.1541985705997
3	6.64644420676667	7.578349903714414
4	3.601699579713624	5.475597440075266
5	2.1709831140576425	4.125631982445159
6	1.476184719433151	3.36632460255711
7	1.0247412497626958	2.7263166373459735
8	0.7747489483777676	2.35567307297496
9	0.5739731725206899	1.9633518621633996
>10	3.1961873128495966	22.06472330835986
>50	0.21276910388159037	5.511103473510153
>100	0.0949503456588292	6.412460708130144
>500	0.0035317501288742938	0.918780215396408
>1k	0.0016408898902235548	1.082296690400208
>5k	7.134303806529908E-5	0.2063615707519667
>10k+	3.567151903264954E-5	0.14590783946078875
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10762	0.14552375842352958	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9190	0.12426717523808185	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.704399896367396E-5
2	0.0	0.0	0.0	0.0	2.704399896367396E-5
3	0.0	0.0	0.0	0.0	2.704399896367396E-5
4	0.0	0.0	0.0	0.0	2.704399896367396E-5
5	0.0	0.0	0.0	0.0	2.704399896367396E-5
6	0.0	0.0	0.0	0.0	2.704399896367396E-5
7	0.0	0.0	0.0	0.0	2.704399896367396E-5
8	0.0	0.0	0.0	0.0	2.704399896367396E-5
9	0.0	0.0	0.0	0.0	2.704399896367396E-5
10-11	0.0	0.0	0.0	6.76099974091849E-6	2.704399896367396E-5
12-13	0.0	0.0	0.0	1.352199948183698E-5	4.732699818642943E-5
14-15	0.0	0.0	0.0	1.352199948183698E-5	1.0817599585469584E-4
16-17	0.0	0.0	0.0	2.704399896367396E-5	1.487419943002068E-4
18-19	0.0	0.0	0.0	4.0565998445510936E-5	1.487419943002068E-4
20-21	0.0	0.0	0.0	5.408799792734792E-5	1.6226399378204375E-4
22-23	0.0	0.0	0.0	8.789299663194036E-5	1.960689924866362E-4
24-25	0.0	0.0	0.0	2.5691799015490263E-4	2.2987399119122866E-4
26-27	0.0	0.0	0.0	7.234269722782784E-4	2.636789898958211E-4
28-29	0.0	0.0	0.0	0.0029748398860041355	2.704399896367396E-4
30-31	0.0	0.0	0.0	0.012656591514999413	2.704399896367396E-4
32-33	0.0	0.0	0.0	0.03428502968619766	2.704399896367396E-4
34-35	0.0	0.0	0.0	0.06538562849442271	2.974839886004136E-4
36-37	0.0	0.0	0.0	0.11973730541166645	2.974839886004136E-4
38-39	0.0	0.0	0.0	0.21251174385654997	2.974839886004136E-4
40-41	0.0	0.0	0.0	0.3166108568674719	2.974839886004136E-4
42-43	0.0	0.0	0.0	0.4233940867755386	2.974839886004136E-4
44-45	0.0	0.0	0.0	0.5538205327775971	2.974839886004136E-4
46-47	0.0	0.0	0.0	0.7122916057049857	3.245279875640875E-4
48-49	0.0	0.0	0.0	0.8834733581453009	3.786159854914354E-4
50-51	0.0	0.0	0.0	1.0689072980394725	3.921379849732724E-4
52-53	0.0	0.0	0.0	1.2550849479051447	4.4622598290062034E-4
54-55	0.0	0.0	0.0	1.4670558117824213	4.800309816052128E-4
56-57	0.0	0.0	0.0	1.7187543101373348	4.867919813461313E-4
58-59	0.0	0.0	0.0	1.996016148512661	4.935529810870498E-4
60-61	0.0	0.0	0.0	2.298117899936122	5.138359803098053E-4
62-63	0.0	0.0	0.0	2.6071902420924697	5.408799792734792E-4
64-65	0.0	0.0	0.0	2.937965393417166	5.408799792734792E-4
66-67	0.0	0.0	0.0	3.320624456753671	5.408799792734792E-4
68-69	0.0	0.0	0.0	3.751996523223493	5.476409790143977E-4
70-71	0.0	0.0	0.0	4.220188994282358	5.679239782371532E-4
72-73	0.0	0.0	0.0	4.700253780886275	6.017289769417456E-4
74-75	0.0	0.0	0.0	5.18935126314406	6.152509764235826E-4
76-77	0.0	0.0	0.0	5.736525733176334	6.220119761645011E-4
78-79	0.0	0.0	0.0	6.351411615613907	6.220119761645011E-4
80-81	0.0	0.0	0.0	7.003002965644926	6.35533975646338E-4
82-83	0.0	0.0	6.76099974091849E-6	7.681043346662419	6.76099974091849E-4
84-85	0.0	0.0	1.352199948183698E-5	8.36444520047446	6.76099974091849E-4
86-87	0.0	0.0	1.352199948183698E-5	9.084586326878652	6.896219735736859E-4
88-89	0.0	0.0	1.352199948183698E-5	9.85576624032704	7.031439730555229E-4
90-91	0.0	0.0	1.352199948183698E-5	10.693751592215438	7.031439730555229E-4
92-93	0.0	0.0	1.352199948183698E-5	11.545847150563137	7.166659725373599E-4
94-95	0.0	0.0	1.352199948183698E-5	12.393744128071724	7.301879720191969E-4
96-97	0.0	0.0	1.352199948183698E-5	13.255413262052564	7.301879720191969E-4
98-99	0.0	0.0	1.352199948183698E-5	14.165173387190556	7.301879720191969E-4
100-101	0.0	0.0	1.352199948183698E-5	15.138453104894477	7.437099715010339E-4
102-103	0.0	0.0	1.352199948183698E-5	16.143212037392114	7.775149702056263E-4
104-105	0.0	0.0	1.352199948183698E-5	17.134388121410247	7.842759699465448E-4
106-107	0.0	0.0	1.352199948183698E-5	18.108749599072716	7.842759699465448E-4
108-109	0.0	0.0	1.352199948183698E-5	19.110871741671396	7.977979694283818E-4
110-111	0.0	0.0	1.352199948183698E-5	20.16044934145158	8.316029681329743E-4
112-113	0.0	0.0	1.352199948183698E-5	21.238943737123677	8.383639678738927E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11165	0.0	54.81047	1
GTATCAA	18755	0.0	48.40302	1
TCAACGC	23470	0.0	38.06286	4
CAACGCA	24095	0.0	37.02658	5
ATCAACG	24235	0.0	36.910553	3
TATCAAC	24765	0.0	36.2655	2
AACGCAG	25130	0.0	35.577286	6
GTACATG	28405	0.0	32.820038	1
TACATGG	29150	0.0	31.075905	2
ACGCAGA	29220	0.0	30.47723	7
ACATGGG	28960	0.0	30.147543	3
CGCAGAG	30475	0.0	29.086084	8
CATGGGG	21300	0.0	28.118967	4
GCAGAGT	32780	0.0	26.35013	9
AGAGTAC	30340	0.0	23.84178	10-11
GAGTACT	18420	0.0	23.092028	12-13
CAGAGTA	32375	0.0	22.407465	10-11
GTACTTT	19385	0.0	21.928257	14-15
AGTACTT	19665	0.0	21.78132	12-13
ATGGGGA	10640	0.0	19.604021	5
>>END_MODULE