FastQCFastQC Report
Mon 15 Aug 2016
SRR2921071_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921071_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2600461
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA52220.2008105485911921No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG123050.028.3524591
TACATGG119350.028.3262182
CATGGGG70150.027.8297214
ACATGGG124800.026.2784463
GTATCAA105600.023.7192971
TACGAGA3603.6379788E-1223.1466544
AACGCAG112700.021.7026986
TCAACGC116150.020.8050864
ATGGGGG34350.020.7930015
GTGTAGC20100.020.7691361
TATCAAC120900.020.627682
CAACGCA118250.020.5866095
GGTATCA84650.020.2899951
ATCAACG121950.020.0596183
TCTATAC9000.019.8399893
ACGCAGA125450.019.2115967
CGCAGAG127000.018.836198
GTATAGA10200.018.7096771
ATGGGGC33150.018.3138355
ATACTCG3253.206187E-718.3110166
Produced by FastQC (version 0.11.5)