##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921071_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2600461
Sequences flagged as poor quality	0
Sequence length	125
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.8457019736116	31.0	31.0	34.0	25.0	34.0
2	30.04293507958781	31.0	31.0	34.0	25.0	34.0
3	30.199624220474753	31.0	31.0	34.0	26.0	34.0
4	33.557812249443465	37.0	35.0	37.0	28.0	37.0
5	33.722029286345766	37.0	35.0	37.0	28.0	37.0
6	33.67915150429097	37.0	35.0	37.0	28.0	37.0
7	33.61381308929455	37.0	35.0	37.0	28.0	37.0
8	33.52417129116722	37.0	35.0	37.0	27.0	37.0
9	34.978703775984336	39.0	35.0	39.0	27.0	39.0
10-11	34.975249965294616	39.0	35.0	39.0	27.0	39.0
12-13	34.905643653183034	39.0	35.0	39.0	27.0	39.0
14-15	36.13561960744653	40.0	36.5	41.0	27.0	41.0
16-17	36.11208743372809	40.0	36.0	41.0	27.0	41.0
18-19	36.15678777724411	40.0	36.5	41.0	27.0	41.0
20-21	36.206949460114956	40.0	37.0	41.0	27.0	41.0
22-23	36.14637039355714	40.0	36.0	41.0	27.0	41.0
24-25	36.13210446147818	40.0	36.0	41.0	27.0	41.0
26-27	36.06325032369261	40.0	36.0	41.0	27.0	41.0
28-29	35.92866591731236	40.0	36.0	41.0	27.0	41.0
30-31	35.81457422357036	40.0	36.0	41.0	27.0	41.0
32-33	35.670357486614876	40.0	35.5	41.0	26.0	41.0
34-35	35.53503955644787	40.0	35.0	41.0	25.5	41.0
36-37	35.400809318040146	40.0	35.0	41.0	25.0	41.0
38-39	35.25884391267549	40.0	35.0	41.0	24.5	41.0
40-41	35.124964381315465	40.0	35.0	41.0	24.0	41.0
42-43	34.93920577928298	40.0	35.0	41.0	23.5	41.0
44-45	34.76073107806654	39.5	35.0	41.0	23.0	41.0
46-47	34.57544835319584	39.0	34.5	41.0	21.5	41.0
48-49	34.34049789633454	39.0	34.0	41.0	19.5	41.0
50-51	34.17066993121604	39.0	34.0	41.0	18.5	41.0
52-53	33.99358825223682	39.0	33.5	41.0	17.5	41.0
54-55	33.76552830440449	38.0	33.0	40.5	14.5	41.0
56-57	33.52795100561016	38.0	33.0	40.0	12.5	41.0
58-59	33.25380230659103	38.0	33.0	40.0	9.0	41.0
60-61	32.95574630805846	37.0	32.5	40.0	8.0	41.0
62-63	32.668800454996244	37.0	32.0	40.0	7.0	41.0
64-65	32.35051612002641	36.0	32.0	39.5	4.0	41.0
66-67	32.001067887578394	36.0	31.5	39.0	2.0	41.0
68-69	31.62566021947647	35.0	31.0	39.0	2.0	40.5
70-71	31.262012966162537	35.0	31.0	38.0	2.0	40.0
72-73	30.89664063410295	35.0	31.0	37.0	2.0	40.0
74-75	30.836418235074476	35.0	31.0	37.0	2.0	39.0
76-77	30.85670982952638	35.0	31.0	37.0	2.0	39.0
78-79	30.660857440276935	35.0	31.0	36.0	2.0	39.0
80-81	30.431997057444814	35.0	31.0	36.0	2.0	37.5
82-83	30.172519603254962	35.0	31.0	35.5	2.0	37.0
84-85	29.91078581836067	35.0	31.0	35.0	2.0	37.0
86-87	29.75468926471114	35.0	31.0	35.0	2.0	36.0
88-89	29.57085974371467	35.0	31.0	35.0	2.0	36.0
90-91	29.420949400894685	35.0	31.0	35.0	2.0	36.0
92-93	29.302648453485748	35.0	31.0	35.0	2.0	35.5
94-95	29.18000346861576	35.0	30.5	35.0	2.0	35.0
96-97	29.07667928878764	35.0	30.0	35.0	2.0	35.0
98-99	28.974469334475693	35.0	30.0	35.0	2.0	35.0
100-101	28.842123761902215	35.0	30.0	35.0	2.0	35.0
102-103	27.60155776225831	33.0	27.5	34.5	2.0	35.0
104-105	28.50712931284107	34.0	29.0	35.0	2.0	35.0
106-107	28.622395221462654	35.0	30.0	35.0	2.0	35.0
108-109	28.55484912098278	35.0	30.0	35.0	2.0	35.0
110-111	28.451868149531947	35.0	29.0	35.0	2.0	35.0
112-113	28.329742880204705	35.0	29.0	35.0	2.0	35.0
114-115	28.18061874413806	35.0	29.0	35.0	2.0	35.0
116-117	28.02394729242238	34.5	29.0	35.0	2.0	35.0
118-119	27.812959702145122	34.0	29.0	35.0	2.0	35.0
120-121	27.597970898236888	34.0	29.0	35.0	2.0	35.0
122-123	27.290133749362134	34.0	28.0	35.0	2.0	35.0
124-125	26.051629884085937	33.0	23.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	89837.0
3	13630.0
4	9432.0
5	10195.0
6	15837.0
7	15503.0
8	12195.0
9	13252.0
10	14226.0
11	15234.0
12	16054.0
13	16422.0
14	16863.0
15	14502.0
16	10864.0
17	9776.0
18	9320.0
19	9083.0
20	9542.0
21	10036.0
22	12022.0
23	15683.0
24	21081.0
25	27051.0
26	29271.0
27	32472.0
28	37133.0
29	44193.0
30	52219.0
31	63759.0
32	80098.0
33	104576.0
34	143510.0
35	216660.0
36	407547.0
37	711335.0
38	269538.0
39	510.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.23216417003932	18.700044154537025	12.33743321385257	24.730358461571093
2	16.185424867210358	21.210016396918565	38.16249073079615	24.442068005074923
3	19.017322131611255	24.98985918311583	29.209632044211652	26.783186641061263
4	12.660525883313314	17.209344572149714	36.061328806195846	34.068800738341125
5	13.545775482742586	38.51059554931503	33.963993299646816	13.979635668295568
6	32.17290728078278	36.88477660810247	17.047505262383783	13.894810848730963
7	27.600459366011766	33.51647145921086	20.8336653842763	18.049403790501074
8	25.537864986687158	35.71044684641954	19.916095987976092	18.835592178917203
9	25.975346021593886	16.5862051839038	18.954810536597684	38.48363825790462
10-11	24.531528381018518	26.857859042888464	27.629774091623222	20.980838484469796
12-13	25.196572182952902	24.79565306919718	27.132561375283615	22.875213372566304
14-15	22.883278282395338	26.1854877233393	24.71676128807262	26.214472706192737
16-17	22.715680181656616	28.46090939552945	25.709730881297492	23.113679541516436
18-19	22.754020837475046	27.715454553677464	27.113524536293337	22.41700007255415
20-21	24.008152976662096	26.88001610426511	26.5564489325632	22.555381986509598
22-23	23.49710488824784	26.8793596965655	26.264990275226108	23.358545139960547
24-25	23.145784160810866	26.85709282264792	26.64531220081537	23.351810815725845
26-27	23.004833716495742	26.96663359505138	26.69593392349005	23.33259876496282
28-29	22.97597958510105	27.29134050715859	26.64892936287156	23.083750544868803
30-31	23.37005217434143	27.17151256325504	26.364034189665812	23.094401072737714
32-33	22.91407450603402	27.300563611501637	26.498420405873414	23.286941476590926
34-35	23.171597241413284	27.286595574837712	26.48892939646871	23.052877787280295
36-37	23.282339180206154	27.027805650270714	26.442252364286617	23.247602805236514
38-39	23.03769325383814	27.220927833940635	26.50177263203915	23.23960628018208
40-41	23.155941275253678	27.280052592542127	26.531290137705167	23.03271599449903
42-43	23.07241431664313	27.152063772521174	26.44123092234507	23.33429098849063
44-45	23.08858533243793	27.2448820549134	26.350369738941026	23.316162873707647
46-47	23.070255539406446	27.208499329856977	26.251231866407	23.470013264329573
48-49	22.99311005384929	27.24175304673615	26.423883402154548	23.341253497260013
50-51	22.823669669200484	27.421213792286025	26.264718117527895	23.490398420985592
52-53	22.993403119417565	27.25606061101728	26.052780817723768	23.697755451841388
54-55	22.936215908303634	27.200285588921307	26.074210781559117	23.789287721215945
56-57	23.00166190165045	27.136457811082103	26.06566195553956	23.796218331727886
58-59	23.02556895799358	26.97937582589435	26.258803446956897	23.73625176915517
60-61	23.09064818308948	26.990742495825586	26.239665962416154	23.678943358668775
62-63	22.96271292384624	26.917834950918206	26.34753621091008	23.77191591432548
64-65	22.994487657749087	26.99612177107712	26.226518280247742	23.78287229092605
66-67	23.09925243711734	27.08651744913095	26.032336602064916	23.781893511686793
68-69	23.203048612812278	26.848186879365	26.14491841096657	23.80384609685615
70-71	23.032908195615306	26.7907576949773	26.30687080512536	23.869463304282032
72-73	23.17576732382768	26.75473919743882	26.322790667248984	23.74670281148451
74-75	22.98981660654266	26.958405220733205	26.191865024570816	23.859913148153318
76-77	22.91022815846958	26.685418450908884	26.533280728259513	23.87107266236203
78-79	22.996720169562135	26.760414592118593	26.443341867242392	23.79952337107688
80-81	23.086817759836684	26.579962065052893	26.388978697002308	23.944241478108115
82-83	23.022929088032765	26.618354047905896	26.48361630632677	23.875100557734573
84-85	23.09330311415835	26.55945608564426	26.366531699147277	23.980709101050113
86-87	22.956341382825325	26.454730772406	26.526798829283766	24.062129015484913
88-89	23.031699028444415	26.396116932988118	26.43542398222544	24.136760056342027
90-91	23.054997624261514	26.462589864785933	26.43341514187364	24.04899736907891
92-93	23.197622560280625	26.460483876553525	26.42219641506225	23.919697148103598
94-95	22.903020066804668	26.53441733749753	26.483109072979367	24.07945352271844
96-97	22.9211651377718	26.504361540547194	26.491180029992268	24.08329329168874
98-99	22.82412011181784	26.736128214916842	26.25302480804399	24.186726865221328
100-101	22.759795964123402	26.969320525120068	26.257427381260527	24.013456129496003
102-103	22.72364516912274	27.11014305487201	26.127494571518394	24.03871720448685
104-105	22.64029580253099	27.25993029776703	25.97184550063101	24.12792839907097
106-107	22.59837882776777	27.388419318584205	26.033266361830865	23.979935491817162
108-109	22.517340017540654	27.49916328725816	26.07116071062557	23.912335984575616
110-111	22.623016125028144	27.773749247056063	25.790711414148998	23.8125232137668
112-113	22.75440540650274	27.914566757997584	25.642749564014995	23.688278271484688
114-115	22.77973478754213	27.973959880957693	25.590746662519997	23.65555866898018
116-117	22.73803182481572	27.983268571147065	25.649770766492804	23.628928837544414
118-119	22.857696486982263	27.90117361004343	25.607062403511648	23.634067499462656
120-121	22.949049421460625	28.153516971207004	25.484162927282977	23.4132706800494
122-123	23.05907755607109	28.21780091815242	25.28244589184045	23.44067563393604
124-125	23.227521945443026	28.33624245354711	25.051740505963632	23.384495095046233
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4290.0
1	2923.0
2	1376.5
3	1039.5
4	782.0
5	622.0
6	534.5
7	537.0
8	603.0
9	648.5
10	668.0
11	690.0
12	749.0
13	861.0
14	995.0
15	1146.5
16	1332.0
17	1567.5
18	1849.5
19	2214.0
20	2686.5
21	3233.5
22	4056.0
23	5135.5
24	6200.0
25	7708.5
26	10156.5
27	13065.0
28	15432.0
29	18392.5
30	21626.0
31	24926.0
32	29866.5
33	34700.0
34	39294.5
35	44126.5
36	50672.5
37	57211.5
38	61600.5
39	65562.0
40	70328.5
41	75847.5
42	80041.0
43	85688.0
44	90691.5
45	93990.5
46	98368.5
47	102222.0
48	107615.0
49	111421.0
50	112852.5
51	110950.5
52	104312.0
53	99542.5
54	96094.0
55	90834.5
56	83598.0
57	75459.0
58	69301.5
59	62986.0
60	53532.0
61	43736.0
62	35255.5
63	26682.5
64	19298.0
65	14451.5
66	10944.5
67	8281.0
68	6062.0
69	4582.0
70	3724.5
71	2902.0
72	2239.0
73	1897.0
74	1576.0
75	1151.5
76	791.5
77	558.5
78	444.0
79	333.5
80	214.5
81	151.0
82	113.0
83	77.5
84	56.0
85	45.0
86	39.0
87	31.5
88	22.5
89	14.0
90	15.0
91	16.0
92	15.0
93	13.0
94	9.0
95	8.0
96	11.5
97	11.0
98	10.5
99	18.5
100	29.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.28056563816953994
2	0.06929540569922026
3	0.07887063101503926
4	0.08475420319704853
5	0.09186832642366104
6	0.1251316593480925
7	0.114133609386951
8	0.11417206410709486
9	0.12120927789341968
10-11	0.09888631284991392
12-13	0.08867658465172137
14-15	0.09859790244883503
16-17	0.09969386197293481
18-19	0.09234901042545918
20-21	0.09196446322402066
22-23	0.07471752123950332
24-25	0.08665771184416916
26-27	0.09857867508876311
28-29	0.09069545745927357
30-31	0.08648466560352185
32-33	0.095502297477255
34-35	0.08917649601359143
36-37	0.08921495073373528
38-39	0.07940899709705317
40-41	0.09129150562150326
42-43	0.0807741396621599
44-45	0.07714016860856594
46-47	0.0677572168934662
48-49	0.06996836330173765
50-51	0.07221796443015296
52-53	0.08411970031467497
54-55	0.08925340545387914
56-57	0.09742503348444757
58-59	0.06779567161361005
60-61	0.08423506447510654
62-63	0.09033013761790698
64-65	0.08113945950352648
66-67	0.07500593164058218
68-69	0.08323524175136639
70-71	0.08177396238590004
72-73	0.06368101655821795
74-75	0.09069545745927357
76-77	0.09559843427761462
78-79	0.07704403180820632
80-81	0.08956104321502995
82-83	0.09425251907257982
84-85	0.09813644580710881
86-87	0.08546561551970977
88-89	0.06500770440318082
90-91	0.08927263281395106
92-93	0.06533456952440356
94-95	0.09379106243085361
96-97	0.08169705294561233
98-99	0.07494824956036641
100-101	0.09184909906358911
102-103	0.06414247319994416
104-105	0.07058363882403929
106-107	0.09002249985675617
108-109	0.09600220883912507
110-111	0.0894456790545984
112-113	0.0782361281326657
114-115	0.09202214530423644
116-117	0.08602320896179562
118-119	0.07789003565137105
120-121	0.11094186761501133
122-123	0.088772721452081
124-125	0.11086495817472362
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2600461.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.25076735305495
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.2161193428746	31.087970981024704
2	14.57583261178008	13.482868862090235
3	6.62676597477955	9.194790342080083
4	3.5762605950537205	6.616191871029099
5	2.174752186010249	5.029197870285387
6	1.403123008296908	3.893730949467533
7	0.9677341267377324	3.1330912168751066
8	0.6574886356571308	2.4327483140044377
9	0.4946877775179215	2.0591720379343075
>10	2.1889898923041784	16.88065329529087
>50	0.08400251963357482	2.6189502210885327
>100	0.032239603270488394	2.462795163845841
>500	0.0013358173892327049	0.411896733340009
>1k	5.844201077819894E-4	0.494299248470406
>5k	8.348858682599848E-5	0.2016428931733818
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5222	0.2008105485911921	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.845472014385142E-5	3.845472014385142E-5
5	0.0	0.0	0.0	3.845472014385142E-5	7.690944028770284E-5
6	0.0	0.0	0.0	3.845472014385142E-5	7.690944028770284E-5
7	0.0	0.0	0.0	3.845472014385142E-5	7.690944028770284E-5
8	0.0	0.0	0.0	3.845472014385142E-5	7.690944028770284E-5
9	0.0	0.0	0.0	3.845472014385142E-5	7.690944028770284E-5
10-11	0.0	0.0	0.0	3.845472014385142E-5	1.1536416043155425E-4
12-13	0.0	0.0	0.0	3.845472014385142E-5	2.1150096079118277E-4
14-15	0.0	0.0	0.0	3.845472014385142E-5	2.884104010788856E-4
16-17	0.0	0.0	0.0	3.845472014385142E-5	3.4609248129466275E-4
18-19	0.0	0.0	0.0	5.768208021577713E-5	3.8454720143851415E-4
20-21	0.0	0.0	0.0	1.9227360071925707E-4	3.8454720143851415E-4
22-23	0.0	0.0	0.0	4.422292816542913E-4	3.8454720143851415E-4
24-25	0.0	0.0	0.0	9.805953636682111E-4	3.8454720143851415E-4
26-27	0.0	0.0	0.0	0.0021342369679837538	4.230019215823656E-4
28-29	0.0	0.0	0.0	0.005787435381649639	4.230019215823656E-4
30-31	0.0	0.0	0.0	0.01788144486689091	4.230019215823656E-4
32-33	0.0	0.0	0.0	0.0482414464204616	4.230019215823656E-4
34-35	0.0	0.0	0.0	0.09952081573228747	4.61456641726217E-4
36-37	0.0	0.0	0.0	0.18164087059948217	4.61456641726217E-4
38-39	0.0	0.0	0.0	0.3170783949461269	4.999113618700684E-4
40-41	0.0	0.0	0.0	0.47816521762872044	4.999113618700684E-4
42-43	0.0	0.0	0.0	0.6252352948188802	4.999113618700684E-4
44-45	0.0	0.0	0.0	0.8076452598212394	4.999113618700684E-4
46-47	0.0	0.0	0.0	1.0293751761706866	5.191387219419942E-4
48-49	0.0	0.0	0.0	1.2641220152888275	5.383660820139198E-4
50-51	0.0	0.0	0.0	1.5205765439281729	5.383660820139198E-4
52-53	0.0	0.0	0.0	1.768205714294504	5.383660820139198E-4
54-55	0.0	0.0	0.0	2.0460410673338307	5.575934420858455E-4
56-57	0.0	0.0	0.0	2.383846556437493	5.768208021577712E-4
58-59	0.0	0.0	0.0	2.753531008540409	5.768208021577712E-4
60-61	0.0	0.0	0.0	3.1455961077670462	5.768208021577712E-4
62-63	0.0	0.0	0.0	3.54562133406346	7.498670428051025E-4
64-65	0.0	0.0	0.0	3.977140976157689	7.690944028770283E-4
66-67	0.0	0.0	0.0	4.475994833223801	7.690944028770283E-4
68-69	0.0	0.0	0.0	5.049623893609633	7.88321762948954E-4
70-71	0.0	0.0	0.0	5.6818964022148375	8.267764830928055E-4
72-73	0.0	0.0	0.0	6.315495598665006	8.652312032366568E-4
74-75	0.0	0.0	0.0	6.962361673564803	8.844585633085826E-4
76-77	0.0	0.0	0.0	7.678465472083603	8.844585633085826E-4
78-79	0.0	0.0	0.0	8.48328430997427	8.844585633085826E-4
80-81	0.0	3.845472014385142E-5	0.0	9.347784873528195	8.844585633085826E-4
82-83	0.0	3.845472014385142E-5	0.0	10.234569947405479	8.844585633085826E-4
84-85	0.0	3.845472014385142E-5	0.0	11.115702177421618	8.844585633085826E-4
86-87	0.0	3.845472014385142E-5	0.0	12.04638331434311	9.036859233805083E-4
88-89	0.0	3.845472014385142E-5	0.0	13.049417007215258	9.421406435243597E-4
90-91	0.0	3.845472014385142E-5	0.0	14.116746992167927	9.613680035962855E-4
92-93	0.0	3.845472014385142E-5	0.0	15.21009159529791	9.613680035962855E-4
94-95	0.0	3.845472014385142E-5	0.0	16.294764659035454	9.613680035962855E-4
96-97	0.0	3.845472014385142E-5	0.0	17.390166589693138	0.0010767321640278397
98-99	0.0	3.845472014385142E-5	0.0	18.544096604409756	0.0010767321640278397
100-101	0.0	3.845472014385142E-5	0.0	19.77733947942307	0.0010767321640278397
102-103	0.0	3.845472014385142E-5	0.0	21.029636668267663	0.001115186884171691
104-105	0.0	3.845472014385142E-5	0.0	22.250958580036386	0.0011536416043155424
106-107	0.0	3.845472014385142E-5	0.0	23.46060948424145	0.0011536416043155424
108-109	0.0	3.845472014385142E-5	0.0	24.680700844965564	0.0011536416043155424
110-111	0.0	3.845472014385142E-5	0.0	25.963434944804018	0.0011536416043155424
112-113	0.0	7.690944028770284E-5	0.0	27.28421999022481	0.0011536416043155424
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACATG	12305	0.0	28.352459	1
TACATGG	11935	0.0	28.326218	2
CATGGGG	7015	0.0	27.829721	4
ACATGGG	12480	0.0	26.278446	3
GTATCAA	10560	0.0	23.719297	1
TACGAGA	360	3.6379788E-12	23.146654	4
AACGCAG	11270	0.0	21.702698	6
TCAACGC	11615	0.0	20.805086	4
ATGGGGG	3435	0.0	20.793001	5
GTGTAGC	2010	0.0	20.769136	1
TATCAAC	12090	0.0	20.62768	2
CAACGCA	11825	0.0	20.586609	5
GGTATCA	8465	0.0	20.289995	1
ATCAACG	12195	0.0	20.059618	3
TCTATAC	900	0.0	19.839989	3
ACGCAGA	12545	0.0	19.211596	7
CGCAGAG	12700	0.0	18.83619	8
GTATAGA	1020	0.0	18.709677	1
ATGGGGC	3315	0.0	18.313835	5
ATACTCG	325	3.206187E-7	18.311016	6
>>END_MODULE