FastQCFastQC Report
Mon 15 Aug 2016
SRR2921071_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921071_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2600461
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84140.3235580152903658No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73610.28306519497889027No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53520.20580966220989277No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT28870.11101877705529904No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA95200.035.964671
GTATCAA132600.033.288121
TACCGTA2353.4197E-1027.8465777
TCAACGC161400.026.9199074
TATCAAC161900.026.9103952
ATCAACG162300.026.7706263
CAACGCA162900.026.6354355
AACGCAG164700.026.3144266
CATGGGG65150.025.9808564
GAGTACT102400.025.53324112-13
GGTCGAC2351.0437361E-825.3150717
GTACATG120050.025.2904591
TACATGG118900.025.0632672
GTATAGG6200.025.0139791
ACATGGG122000.024.0355913
ACGCAGA181450.023.9010817
AGTACTT107300.023.89596212-13
CGCAGAG184750.023.3780088
GTACTTT113300.022.84229514-15
CTATACT8850.022.2238984
Produced by FastQC (version 0.11.5)