FastQCFastQC Report
Mon 15 Aug 2016
SRR2921070_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921070_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5965937
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA104350.041.0516661
GGTATCA71350.038.3811071
TCAACGC116400.035.2336584
CAACGCA121600.033.775915
GTACATG197700.033.4675331
ATCAACG124250.033.295073
AACGCAG126600.032.5321586
TACATGG200050.031.9555512
ACATGGG203150.030.3552953
TATCAAC138900.030.2540192
CATGGGG143400.028.475184
ACGCAGA143300.028.2809547
CGCAGAG152050.026.574758
GCAGAGT179700.021.5253949
AGAGTAC153050.020.23563210-11
CAGAGTA163600.019.29440710-11
ATGGGGG74550.019.0029415
ATGGGGC66950.017.5148815
ATGGGGA77900.017.4216355
GTATAGG16000.017.1528641
Produced by FastQC (version 0.11.5)