##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921070_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5965937
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.128588518450663	31.0	31.0	34.0	26.0	34.0
2	30.33505348782597	31.0	31.0	34.0	26.0	34.0
3	30.483381403457663	31.0	31.0	34.0	26.0	34.0
4	33.92496501387795	37.0	35.0	37.0	30.0	37.0
5	34.010384286659416	37.0	35.0	37.0	30.0	37.0
6	33.98675899527601	37.0	35.0	37.0	30.0	37.0
7	33.90392456373576	37.0	35.0	37.0	29.0	37.0
8	33.83833151439581	37.0	35.0	37.0	29.0	37.0
9	35.36047866412267	39.0	37.0	39.0	29.0	39.0
10-11	35.31474980376092	39.0	35.0	39.0	29.0	39.0
12-13	35.241405583062644	39.0	35.0	39.0	28.0	39.0
14-15	36.4832444593364	40.0	37.0	41.0	28.5	41.0
16-17	36.44857027487887	40.0	37.0	41.0	28.0	41.0
18-19	36.48085020006078	40.0	37.0	41.0	28.0	41.0
20-21	36.51963103197369	40.0	37.0	41.0	28.0	41.0
22-23	36.451147573298215	40.0	37.0	41.0	28.0	41.0
24-25	36.42985452243294	40.0	37.0	41.0	28.0	41.0
26-27	36.37296287238702	40.0	37.0	41.0	28.0	41.0
28-29	36.25963683491797	40.0	36.0	41.0	28.0	41.0
30-31	36.1725992916117	40.0	36.0	41.0	28.0	41.0
32-33	36.06696450197178	40.0	36.0	41.0	27.0	41.0
34-35	35.965516397508054	40.0	36.0	41.0	27.0	41.0
36-37	35.8607631123158	40.0	36.0	41.0	27.0	41.0
38-39	35.74175079287629	40.0	35.0	41.0	26.5	41.0
40-41	35.61968136773821	40.0	35.0	41.0	26.0	41.0
42-43	35.45856023957343	40.0	35.0	41.0	26.0	41.0
44-45	35.29300896070475	40.0	35.0	41.0	25.0	41.0
46-47	35.1302108118138	39.0	35.0	41.0	24.5	41.0
48-49	34.91859258654592	39.0	34.5	41.0	24.0	41.0
50-51	34.75684431464831	39.0	34.0	41.0	24.0	41.0
52-53	34.58212314343916	39.0	34.0	41.0	23.0	41.0
54-55	34.35998201120796	38.5	34.0	40.5	22.5	41.0
56-57	34.12364009207606	38.0	33.5	40.0	22.0	41.0
58-59	33.852834433216444	38.0	33.0	40.0	21.5	41.0
60-61	33.55914267951539	37.5	33.0	40.0	20.0	41.0
62-63	33.275396974523865	37.0	33.0	40.0	19.5	41.0
64-65	32.95301551122648	36.5	32.0	40.0	18.0	41.0
66-67	32.61023423478994	36.0	32.0	39.0	17.5	41.0
68-69	32.249495343313214	35.5	32.0	39.0	15.0	40.5
70-71	31.88819493065381	35.0	31.0	38.5	13.0	40.0
72-73	31.526835851602186	35.0	31.0	37.5	11.5	40.0
74-75	31.466331273695985	35.0	31.0	37.0	12.0	39.0
76-77	31.48603547104168	35.0	32.0	37.0	13.0	39.0
78-79	31.28518286398264	35.0	32.0	36.0	11.0	39.0
80-81	31.0492664438126	35.0	32.0	36.0	9.0	37.5
82-83	30.78950800184447	35.0	32.0	35.5	7.0	37.0
84-85	30.52543012103547	35.0	31.5	35.0	6.0	37.0
86-87	30.365835157159722	35.0	32.0	35.0	2.0	36.0
88-89	30.18457871747556	35.0	31.0	35.0	2.0	36.0
90-91	30.035900731100583	35.0	31.0	35.0	2.0	36.0
92-93	29.919712276546	35.0	31.0	35.0	2.0	35.5
94-95	29.806823471317244	35.0	31.0	35.0	2.0	35.0
96-97	29.708247505798333	35.0	31.0	35.0	2.0	35.0
98-99	29.609442741349767	35.0	31.0	35.0	2.0	35.0
100-101	29.48013891195968	35.0	31.0	35.0	2.0	35.0
102-103	28.210359914963902	33.0	28.0	34.5	2.0	35.0
104-105	29.148429492299364	34.0	30.0	35.0	2.0	35.0
106-107	29.278085906706693	35.0	31.0	35.0	2.0	35.0
108-109	29.209289504733288	35.0	31.0	35.0	2.0	35.0
110-111	29.108451027893857	35.0	30.0	35.0	2.0	35.0
112-113	28.998095437481155	35.0	30.0	35.0	2.0	35.0
114-115	28.861023591097258	35.0	30.0	35.0	2.0	35.0
116-117	28.72822425044046	35.0	30.0	35.0	2.0	35.0
118-119	28.535987054506272	35.0	30.0	35.0	2.0	35.0
120-121	28.339020425458735	34.5	30.0	35.0	2.0	35.0
122-123	28.057241553170947	34.0	29.0	35.0	2.0	35.0
124-125	26.8446378163229	33.0	26.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	181773.0
3	28549.0
4	18068.0
5	16435.0
6	19588.0
7	21743.0
8	23238.0
9	24649.0
10	27362.0
11	29838.0
12	31057.0
13	30811.0
14	31208.0
15	28099.0
16	23552.0
17	21167.0
18	20384.0
19	19995.0
20	20619.0
21	22225.0
22	27087.0
23	34374.0
24	47249.0
25	61670.0
26	70168.0
27	76707.0
28	89558.0
29	105998.0
30	125011.0
31	151549.0
32	190866.0
33	248909.0
34	338440.0
35	510870.0
36	961801.0
37	1663454.0
38	621141.0
39	725.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.55703313621861	17.99998285422952	11.971429094926886	25.47155491462499
2	15.959578610350771	20.096335174079776	39.035353368468705	24.908732847100744
3	18.620730377404097	24.43986902920473	29.51086913673109	27.42853145666008
4	12.591698997960558	16.3271817898611	36.87107895512505	34.210040257053286
5	13.65392920020817	37.79631193622845	34.71213782139361	13.837621042169769
6	32.79372256913351	36.474644447636834	17.304924499246134	13.426708483983528
7	28.363049830145098	32.222189406504626	21.49721674237029	17.917544020979985
8	26.135467206153535	35.54625981857092	19.995710864618207	18.32256211065734
9	26.541373420548915	15.44860656560283	19.117521196118595	38.89249881772966
10-11	25.06911773588451	25.85675288417012	28.21730099393972	20.856828386005652
12-13	25.595167094718914	23.435570965324498	27.91450899983408	23.054752940122512
14-15	23.03326819862013	24.823674461887805	24.95023876250029	27.192818576991773
16-17	23.306462092557503	27.49555187048785	25.744747625014693	23.453238411939957
18-19	23.32601436865856	27.004421157973457	26.867747356889076	22.80181711647891
20-21	24.225819478028445	26.232314469382494	26.51477797594971	23.027088076639355
22-23	23.89546659599254	26.101346358842836	26.289155575609712	23.714031469554914
24-25	23.53732897552866	26.21642262708854	26.523537974845464	23.72271042253734
26-27	23.499820015229286	26.326684061419474	26.534562067397943	23.638933855953294
28-29	23.57970271328796	26.463904404406964	26.421157098112197	23.535235784192878
30-31	23.76557378768021	26.36433446920052	26.25296775884666	23.61712398427261
32-33	23.393964041343672	26.456982077714713	26.337477246812036	23.811576634129576
34-35	23.757468033983564	26.38102375972443	26.405064066242534	23.456444140049467
36-37	23.881565029701516	26.188559703409286	26.26923669899059	23.66063856789861
38-39	23.617605800663018	26.45769126734338	26.288905057757106	23.63579787423649
40-41	23.77976063403904	26.314972279707494	26.41755241892088	23.487714667332583
42-43	23.60046415719278	26.330419699924974	26.325202754245375	23.743913388636873
44-45	23.62831827976036	26.369840753436	26.26187371169017	23.739967255113463
46-47	23.682786180934905	26.35641134490787	26.22489752371266	23.735904950444567
48-49	23.57713813484576	26.39529975690162	26.201069290712436	23.826492817540185
50-51	23.54175000901565	26.56438306533789	26.138584383551766	23.7552825420947
52-53	23.70263301079929	26.451325614335218	26.09095230230863	23.755089072556864
54-55	23.51679663941391	26.42372373808164	26.12357434701172	23.935905275492736
56-57	23.491302726976528	26.49811907776257	26.125046851086864	23.88553134417404
58-59	23.484189018607804	26.23624608822161	26.30639728606694	23.973167607103647
60-61	23.523296192609607	26.266522276340172	26.323767879605242	23.886413651444986
62-63	23.364107970308314	26.342788399192667	26.348358064380296	23.94474556611872
64-65	23.37236380746532	26.21831316388396	26.32702144264718	24.082301586003545
66-67	23.28816157534231	26.317091198329877	26.265185093613404	24.12956213271441
68-69	23.420502546517284	26.193892283853852	26.303407372813787	24.08219779681508
70-71	23.23522169057727	26.010203691365007	26.457676185951822	24.2968984321059
72-73	23.297552355717997	26.006836860277076	26.402005841285686	24.29360494271924
74-75	23.111544721011956	26.135998943767884	26.407849535910465	24.34460679930969
76-77	23.141229544775843	25.9860785359814	26.540538001335438	24.332153917907316
78-79	23.10708598125135	26.02485875440863	26.55693050290845	24.311124761431575
80-81	23.046155959925244	26.028268001720207	26.53236892792954	24.39320711042501
82-83	23.074788565442645	25.932354195234232	26.6572079674861	24.335649271837028
84-85	22.992508770749527	25.93467559509479	26.591129140363368	24.481686493792317
86-87	22.88764122883397	25.889328245582266	26.678073370340393	24.54495715524337
88-89	22.912947454364804	25.762652366106682	26.620083847062638	24.70431633246588
90-91	22.83189017756562	25.81581289386913	26.678644733718677	24.67365219484658
92-93	22.86057180675982	25.7003176145149	26.78499877311603	24.65411180560925
94-95	22.694029224755482	25.604375325045716	26.862566477091615	24.839028973107187
96-97	22.73960667456865	25.46053980173027	26.883567446028383	24.916286077672698
98-99	22.67539391808624	25.490710801214888	26.775015352141473	25.0588799285574
100-101	22.59690991490657	25.560047725601947	26.871662957396914	24.97137940209457
102-103	22.492268058527944	25.474432599609546	26.906728634012495	25.126570707850014
104-105	22.35323588951298	25.509872556391542	26.846343911058344	25.29054764303713
106-107	22.333960531476613	25.53161417517013	26.812242509606765	25.322182783746488
108-109	22.24675020946096	25.479101047589996	26.907352993974705	25.366795748974337
110-111	22.289746514788035	25.622649264372832	26.799065577345704	25.288538643493432
112-113	22.328948870372244	25.5890525666717	26.813584434970572	25.268414127985476
114-115	22.230845272861256	25.704810664062975	26.74200931339798	25.32233474967779
116-117	22.077103896294524	25.640113317774155	26.81701611905496	25.46576666687636
118-119	22.18441243589248	25.537868276735654	26.749059999582325	25.528659287789544
120-121	22.09086595301209	25.660674129400817	26.781938357775648	25.46652155981144
122-123	22.163594321807786	25.673129258918863	26.637950869044037	25.52532555022931
124-125	22.2160968474054	25.71957167158356	26.475663478488777	25.58866800252226
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1760.0
1	1442.0
2	989.0
3	767.5
4	596.5
5	502.5
6	477.0
7	487.5
8	526.0
9	567.0
10	631.0
11	721.0
12	806.0
13	907.5
14	1046.0
15	1174.0
16	1346.5
17	1541.0
18	1868.0
19	2262.5
20	2725.5
21	3445.5
22	4501.0
23	5982.5
24	7985.5
25	10717.5
26	15064.0
27	20679.0
28	26558.5
29	34148.0
30	43683.0
31	54207.0
32	65934.5
33	76473.0
34	87414.0
35	99239.5
36	112257.5
37	126309.5
38	137798.5
39	148489.0
40	160818.5
41	174120.0
42	184814.5
43	197903.5
44	211759.0
45	223715.5
46	236072.0
47	243382.0
48	249433.5
49	254455.0
50	255135.0
51	252665.0
52	246232.0
53	239641.0
54	232523.0
55	219990.5
56	202803.0
57	184123.0
58	167676.5
59	149035.5
60	125793.0
61	104040.5
62	84843.5
63	64926.5
64	47874.0
65	36410.5
66	27545.5
67	21291.0
68	16036.0
69	11672.0
70	8907.5
71	6820.0
72	5098.0
73	3962.5
74	3110.5
75	2394.0
76	1715.0
77	1147.0
78	803.5
79	539.5
80	369.0
81	258.0
82	185.0
83	134.0
84	114.0
85	99.5
86	73.5
87	61.5
88	48.5
89	38.0
90	31.5
91	25.0
92	17.0
93	12.5
94	11.0
95	9.5
96	10.5
97	11.0
98	10.5
99	10.5
100	16.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.28407943295411936
2	0.06778482575327228
3	0.07723849581381767
4	0.08370856078433278
5	0.09069824237165092
6	0.12271333069725679
7	0.11285737680434775
8	0.11220366557675683
9	0.1183887794993477
10-11	0.09750354386913573
12-13	0.0874296862336964
14-15	0.09596983675824938
16-17	0.0980147795727645
18-19	0.09042167223690092
20-21	0.08957520000630244
22-23	0.07210937695118134
24-25	0.08354932343402217
26-27	0.0957184093630221
28-29	0.08929862987155245
30-31	0.08380075082924945
32-33	0.09233252044062819
34-35	0.0857283608593252
36-37	0.08598816916772671
38-39	0.07625792897243132
40-41	0.08768949454209791
42-43	0.07701221115811313
44-45	0.07367660771476467
46-47	0.06413074760930261
48-49	0.06616730951064351
50-51	0.06842177515451471
52-53	0.07974438885291615
54-55	0.08501598323951459
56-57	0.0932460399766206
58-59	0.06394636751946929
60-61	0.0801299108589313
62-63	0.08508303054490854
64-65	0.07659316549940101
66-67	0.070575669840295
68-69	0.07807658713124191
70-71	0.07651773728083285
72-73	0.05952124536346931
74-75	0.08592112186233276
76-77	0.08979310374883274
78-79	0.07266251722068134
80-81	0.08427008196700703
82-83	0.08861139499126457
84-85	0.09219004491666608
86-87	0.08083390756556766
88-89	0.06123095165101475
90-91	0.08451312844906005
92-93	0.061649997309726874
94-95	0.08778168458701457
96-97	0.07648421362813587
98-99	0.07086900180139348
100-101	0.08707768788037822
102-103	0.061297998956408695
104-105	0.06714787635202986
106-107	0.08439579566462066
108-109	0.09049710045546912
110-111	0.08442931931731763
112-113	0.07364308406206771
114-115	0.08665026130849185
116-117	0.08086743121826463
118-119	0.07325756205605255
120-121	0.10377246692346903
122-123	0.0836498943921131
124-125	0.10410770345043872
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	5965937.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.728724441198096
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.49753415029103	33.86526190952286
2	14.914530973110576	14.535321399168389
3	6.085063046683857	8.89552081227525
4	3.0109782977085784	5.8688452706987615
5	1.7763465966610288	4.327955191037767
6	1.1085681510277838	3.2411467173432817
7	0.7329203778043427	2.499999258915663
8	0.5039214549240608	1.964435977360175
9	0.39406679178170845	1.7282134897342074
>10	1.829693976551409	15.653715581701302
>50	0.10331646009847713	3.4463113406632426
>100	0.041646719639539066	3.371443691351673
>500	0.001068374353143607	0.34976118677672496
>1k	3.4462936442215906E-4	0.2520681734507207
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.676182634848474E-5
2	0.0	0.0	0.0	0.0	1.676182634848474E-5
3	0.0	0.0	0.0	0.0	1.676182634848474E-5
4	0.0	0.0	0.0	0.0	1.676182634848474E-5
5	0.0	0.0	0.0	0.0	3.352365269696948E-5
6	0.0	0.0	0.0	0.0	8.38091317424237E-5
7	0.0	0.0	0.0	0.0	8.38091317424237E-5
8	0.0	0.0	0.0	0.0	8.38091317424237E-5
9	0.0	0.0	0.0	0.0	8.38091317424237E-5
10-11	0.0	0.0	0.0	8.38091317424237E-6	1.0057095809090843E-4
12-13	0.0	0.0	0.0	1.676182634848474E-5	1.5085643713636266E-4
14-15	0.0	0.0	0.0	2.514273952272711E-5	1.8438008983333212E-4
16-17	0.0	0.0	0.0	7.542821856818133E-5	2.0114191618181686E-4
18-19	0.0	0.0	0.0	9.219004491666606E-5	2.179037425303016E-4
20-21	0.0	0.0	0.0	1.0057095809090843E-4	2.5980830840151346E-4
22-23	0.0	0.0	0.0	1.5085643713636266E-4	2.8495104792424056E-4
24-25	0.0	0.0	0.0	2.0952282935605922E-4	2.8495104792424056E-4
26-27	0.0	0.0	0.0	4.6933113775757273E-4	3.519983533181795E-4
28-29	0.0	0.0	0.0	0.0018689436378560484	3.687601796666643E-4
30-31	0.0	0.0	0.0	0.007065109805886318	3.771410928409066E-4
32-33	0.0	0.0	0.0	0.021597613250022588	3.85522006015149E-4
34-35	0.0	0.0	0.0	0.04282646632037851	4.5256931140908797E-4
36-37	0.0	0.0	0.0	0.07993714985592373	4.5256931140908797E-4
38-39	0.0	0.0	0.0	0.15329528287006716	4.944738772802998E-4
40-41	0.0	0.0	0.0	0.24282857831049842	5.028547904545422E-4
42-43	0.0	0.0	0.0	0.3320936845293539	5.028547904545422E-4
44-45	0.0	0.0	0.0	0.4373495730846638	5.112357036287845E-4
46-47	0.0	0.0	0.0	0.5697344775849963	5.279975299772693E-4
48-49	0.0	0.0	0.0	0.7134922812627756	5.363784431515117E-4
50-51	1.676182634848474E-5	0.0	0.0	0.8778671313491913	5.531402694999964E-4
52-53	1.676182634848474E-5	0.0	0.0	1.043599689369834	6.201875748939353E-4
54-55	1.676182634848474E-5	0.0	0.0	1.22838206303553	6.201875748939353E-4
56-57	1.676182634848474E-5	0.0	0.0	1.451666351823695	6.285684880681777E-4
58-59	1.676182634848474E-5	0.0	0.0	1.7020964183832312	6.369494012424201E-4
60-61	1.676182634848474E-5	0.0	0.0	1.98109701795376	6.369494012424201E-4
62-63	1.676182634848474E-5	0.0	0.0	2.267950533168553	6.704730539393896E-4
64-65	1.676182634848474E-5	0.0	0.0	2.5686660787735436	6.704730539393896E-4
66-67	1.676182634848474E-5	0.0	0.0	2.9192229150257534	6.704730539393896E-4
68-69	1.676182634848474E-5	0.0	0.0	3.3227471225391754	6.704730539393896E-4
70-71	1.676182634848474E-5	0.0	0.0	3.7740928206248237	7.039967066363592E-4
72-73	1.676182634848474E-5	0.0	0.0	4.238688742438949	7.542821856818132E-4
74-75	1.676182634848474E-5	0.0	0.0	4.7096206346128024	7.794249252045404E-4
76-77	1.676182634848474E-5	0.0	0.0	5.224300893556201	7.878058383787828E-4
78-79	1.676182634848474E-5	0.0	0.0	5.808668445543424	8.129485779015098E-4
80-81	1.676182634848474E-5	0.0	0.0	6.4498585888520115	8.297104042499946E-4
82-83	1.676182634848474E-5	0.0	0.0	7.116685945560605	8.883767964696912E-4
84-85	1.676182634848474E-5	0.0	0.0	7.77863561080179	8.883767964696912E-4
86-87	1.676182634848474E-5	0.0	0.0	8.469440760101893	9.386622755151454E-4
88-89	1.676182634848474E-5	0.0	0.0	9.233478328718522	9.973286677348418E-4
90-91	1.676182634848474E-5	0.0	0.0	10.057263427354329	0.0010140904940833268
92-93	1.676182634848474E-5	0.0	0.0	10.913356946276838	0.0010895187126515082
94-95	1.676182634848474E-5	0.0	0.0	11.757373569315265	0.0011062805389999927
96-97	1.676182634848474E-5	0.0	0.0	12.613768801112046	0.0011900896707424165
98-99	1.676182634848474E-5	0.0	0.0	13.516309340846208	0.0012403751497878706
100-101	1.676182634848474E-5	0.0	0.0	14.496323041963064	0.0012487560629621131
102-103	1.676182634848474E-5	0.0	0.0	15.512198670552504	0.0012990415420075672
104-105	1.676182634848474E-5	0.0	0.0	16.513935698616997	0.0013241842815302945
106-107	1.676182634848474E-5	0.0	0.0	17.494142160736864	0.0013241842815302945
108-109	1.676182634848474E-5	1.676182634848474E-5	0.0	18.496214425328326	0.0013241842815302945
110-111	1.676182634848474E-5	1.676182634848474E-5	0.0	19.548102837827486	0.0013241842815302945
112-113	1.676182634848474E-5	1.676182634848474E-5	0.0	20.647460742545555	0.0013409461078787792
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	10435	0.0	41.051666	1
GGTATCA	7135	0.0	38.381107	1
TCAACGC	11640	0.0	35.233658	4
CAACGCA	12160	0.0	33.77591	5
GTACATG	19770	0.0	33.467533	1
ATCAACG	12425	0.0	33.29507	3
AACGCAG	12660	0.0	32.532158	6
TACATGG	20005	0.0	31.955551	2
ACATGGG	20315	0.0	30.355295	3
TATCAAC	13890	0.0	30.254019	2
CATGGGG	14340	0.0	28.47518	4
ACGCAGA	14330	0.0	28.280954	7
CGCAGAG	15205	0.0	26.57475	8
GCAGAGT	17970	0.0	21.525394	9
AGAGTAC	15305	0.0	20.235632	10-11
CAGAGTA	16360	0.0	19.294407	10-11
ATGGGGG	7455	0.0	19.002941	5
ATGGGGC	6695	0.0	17.514881	5
ATGGGGA	7790	0.0	17.421635	5
GTATAGG	1600	0.0	17.152864	1
>>END_MODULE