FastQCFastQC Report
Mon 15 Aug 2016
SRR2921070_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921070_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5965937
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75950.12730607111674158No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64210.10762768698362052No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83600.052.1660461
GTATCAA132950.045.8156931
TCAACGC162750.036.6950534
CAACGCA167450.035.558315
ATCAACG169600.035.177833
AACGCAG176950.033.9068576
TATCAAC182500.032.952832
GTACATG191500.032.275121
TACATGG197600.030.856962
ACGCAGA200750.029.5435227
ACATGGG201750.029.3357053
CGCAGAG210900.028.1787998
CATGGGG143450.027.9209084
GCAGAGT238700.024.1985559
AGAGTAC212600.023.27978910-11
GAGTACT130350.022.06567212-13
CAGAGTA228700.021.52389310-11
GTACTTT140750.020.2880814-15
AGTACTT148250.020.18389712-13
ATGGGGG72250.018.4786825
Produced by FastQC (version 0.11.5)