FastQCFastQC Report
Mon 15 Aug 2016
SRR2921069_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921069_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2855182
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37720.13211066755114034No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG124350.027.4882471
TACATGG122650.027.0801752
CATGGGG66400.026.9844594
TAGTACG1556.811759E-626.883244
GTATCAA84350.026.3084981
GGTATCA66950.026.1961
ACATGGG129200.024.6033383
AACGCAG94150.022.8833566
TCAACGC95950.022.3963624
TATCAAC97850.022.2640952
CAACGCA98150.022.1365665
ATCAACG99250.021.6516973
ACGCAGA103850.020.457267
GTATAAT12300.019.8846041
GTATAGA8750.019.7709791
CGCAGAG107200.019.5407528
GAGTACT36650.018.7497212-13
ATGGGGA37350.018.0092455
TATACCG2000.00117145717.857845
GCAGAGT116200.017.1054219
Produced by FastQC (version 0.11.5)