##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2921069_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2855182 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.180640323453986 31.0 30.0 34.0 22.0 34.0 2 29.377075436872325 31.0 30.0 34.0 22.0 34.0 3 29.536008562676564 31.0 30.0 34.0 23.0 34.0 4 32.89100449638587 37.0 35.0 37.0 25.0 37.0 5 33.01654710627904 37.0 35.0 37.0 26.0 37.0 6 32.96905206042907 37.0 35.0 37.0 25.0 37.0 7 32.88770838426412 37.0 35.0 37.0 25.0 37.0 8 32.79411365019813 37.0 35.0 37.0 25.0 37.0 9 34.19815339267339 39.0 35.0 39.0 23.0 39.0 10-11 34.16751839287303 39.0 35.0 39.0 23.5 39.0 12-13 34.07506509217276 39.0 35.0 39.0 22.5 39.0 14-15 35.221525633041956 40.0 36.0 41.0 22.5 41.0 16-17 35.18381647824903 40.0 35.5 41.0 22.5 41.0 18-19 35.21590164830123 40.0 35.5 41.0 23.0 41.0 20-21 35.26090823632259 40.0 35.0 41.0 23.5 41.0 22-23 35.178029631736266 40.0 35.0 41.0 23.5 41.0 24-25 35.15584102869799 40.0 35.0 41.0 24.0 41.0 26-27 35.073141572060905 40.0 35.0 41.0 23.0 41.0 28-29 34.93564665930228 40.0 35.0 41.0 22.5 41.0 30-31 34.817618806787095 40.0 35.0 41.0 22.0 41.0 32-33 34.6756152497459 40.0 34.0 41.0 20.0 41.0 34-35 34.5448615885082 40.0 34.0 41.0 18.5 41.0 36-37 34.41270153706489 39.5 34.0 41.0 17.0 41.0 38-39 34.27036822871537 39.0 34.0 41.0 16.0 41.0 40-41 34.133783240437914 39.0 34.0 41.0 15.0 41.0 42-43 33.9544027315947 39.0 33.0 41.0 11.5 41.0 44-45 33.78259459467032 39.0 33.0 41.0 9.0 41.0 46-47 33.601636953441144 39.0 33.0 41.0 8.0 41.0 48-49 33.36935386255587 38.0 33.0 41.0 6.5 41.0 50-51 33.19917521895277 38.0 33.0 40.5 2.0 41.0 52-53 33.02803078752948 38.0 32.5 40.0 2.0 41.0 54-55 32.79755493695323 38.0 32.0 40.0 2.0 41.0 56-57 32.56356127210104 38.0 32.0 40.0 2.0 41.0 58-59 32.29130192050805 37.0 31.0 40.0 2.0 41.0 60-61 31.99724886189392 37.0 31.0 40.0 2.0 41.0 62-63 31.71670825187326 36.0 31.0 40.0 2.0 41.0 64-65 31.40687581387106 36.0 31.0 39.0 2.0 41.0 66-67 31.065720854222253 35.5 30.0 39.0 2.0 41.0 68-69 30.699732276261198 35.0 30.0 39.0 2.0 40.0 70-71 30.33773451219572 35.0 29.5 38.0 2.0 40.0 72-73 29.969964086352462 35.0 29.0 37.0 2.0 39.5 74-75 29.911843273038286 35.0 29.5 37.0 2.0 39.0 76-77 29.930840660945606 35.0 30.0 36.5 2.0 39.0 78-79 29.73375059803543 35.0 30.0 36.0 2.0 39.0 80-81 29.50299525564395 35.0 30.0 36.0 2.0 37.0 82-83 29.244996816315037 35.0 30.0 35.0 2.0 37.0 84-85 28.982176267572434 35.0 29.5 35.0 2.0 36.5 86-87 28.818993850479586 35.0 29.0 35.0 2.0 36.0 88-89 28.636616685030937 35.0 29.0 35.0 2.0 36.0 90-91 28.479070511091763 34.0 29.0 35.0 2.0 36.0 92-93 28.360211012818098 34.0 29.0 35.0 2.0 35.0 94-95 28.242313975081096 34.0 29.0 35.0 2.0 35.0 96-97 28.142639943793426 34.0 29.0 35.0 2.0 35.0 98-99 28.036626386689186 34.0 29.0 35.0 2.0 35.0 100-101 27.90447053813032 34.0 29.0 35.0 2.0 35.0 102-103 26.66049414713318 32.5 25.5 34.5 2.0 35.0 104-105 27.580880308155486 34.0 27.5 35.0 2.0 35.0 106-107 27.72067665038516 34.0 28.5 35.0 2.0 35.0 108-109 27.66080270889912 34.0 28.5 35.0 2.0 35.0 110-111 27.5686858140742 34.0 27.5 35.0 2.0 35.0 112-113 27.45911679185425 34.0 27.0 35.0 2.0 35.0 114-115 27.329620318424535 34.0 27.0 35.0 2.0 35.0 116-117 27.191503553889035 34.0 27.0 35.0 2.0 35.0 118-119 26.993891807947794 34.0 26.5 35.0 2.0 35.0 120-121 26.788418741782486 34.0 25.0 35.0 2.0 35.0 122-123 26.495400643461608 34.0 25.0 35.0 2.0 35.0 124-125 25.260297417117368 32.5 20.5 34.5 2.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 137367.0 3 19668.0 4 12883.0 5 12950.0 6 18388.0 7 18188.0 8 15972.0 9 16935.0 10 17876.0 11 18479.0 12 19154.0 13 19193.0 14 18938.0 15 17033.0 16 13818.0 17 12483.0 18 11736.0 19 11508.0 20 11919.0 21 12810.0 22 14840.0 23 19002.0 24 24980.0 25 31385.0 26 34976.0 27 39193.0 28 44914.0 29 52801.0 30 61696.0 31 74125.0 32 91900.0 33 116684.0 34 158360.0 35 234996.0 36 433960.0 37 729564.0 38 254186.0 39 322.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.81556400058174 18.88292397438951 12.284808156686852 25.0167038683419 2 16.298983398962896 21.111969815658917 37.959542748590586 24.6295040367876 3 18.987982484797584 24.853864715672405 29.148328189913475 27.009824609616533 4 12.754850399778736 16.983611683375383 36.191710107922205 34.06982780892368 5 13.642833453582695 38.32476863391817 34.013320684362604 14.019077228136528 6 32.39582873050241 36.80672598970509 16.9290192621022 13.868426017690302 7 27.82704621617575 33.20308979790921 20.890815288531567 18.079048697383477 8 25.63535586134783 35.589080263283854 20.032631712933497 18.74293216243482 9 26.117340034457108 16.18284922366298 19.184082539965306 38.5157282019146 10-11 24.61394313361147 26.704484100550435 27.83725414577709 20.844318620061003 12-13 25.10513127562553 24.674650427115527 27.333798635922573 22.88641966133637 14-15 22.901906793328116 26.04888007269851 24.782125337618954 26.267087796354417 16-17 22.82805717006362 28.322643572014073 25.744391644245866 23.104907613676442 18-19 22.768007387214563 27.642212294075087 27.168171313824807 22.421609004885546 20-21 23.955202323283952 26.891506930377908 26.6686859380883 22.484604808249845 22-23 23.48735071101592 26.886589179252983 26.285972053568347 23.34008805616275 24-25 23.050381350250685 26.91145295190421 26.66025167451452 23.37791402333059 26-27 23.027534209379052 26.922865894115482 26.62780830161505 23.42179159489041 28-29 23.070983732127864 27.177372565997853 26.72044886075816 23.031194841116122 30-31 23.385450059750433 27.164187318746215 26.315359594545377 23.13500302695798 32-33 23.023514957570214 27.23327590253674 26.38862424991284 23.354584889980202 34-35 23.170688314886597 27.138093289151143 26.59672419640799 23.094494199554262 36-37 23.320763408231716 27.045800006730758 26.385116470165915 23.24832011487161 38-39 23.107729759429212 27.17521892178229 26.44960702302304 23.26744429576545 40-41 23.203943720926073 27.100022804281597 26.607934102111646 23.08809937268068 42-43 23.112852060220483 27.090172984909827 26.449603028550396 23.347371926319294 44-45 23.172461632507705 27.14032140924297 26.382260239977345 23.30495671827198 46-47 23.009120325384192 27.119216119905687 26.320002593540508 23.551660961169617 48-49 22.99349252185019 27.05750322710212 26.47113973571234 23.47786451533535 50-51 22.835000164732058 27.265013224128833 26.34540527240724 23.55458133873187 52-53 22.956592913529352 27.200500567871117 26.135023626242653 23.707882892356874 54-55 22.91441689279067 26.995884283170994 26.269494705096385 23.820204118941955 56-57 22.940980864519226 26.99746509176662 26.185035855252377 23.876518188461777 58-59 22.881912688397634 26.732919990445954 26.436607779820875 23.948559541335545 60-61 23.039206321357895 26.677075367677023 26.357698375404855 23.926019935560234 62-63 22.793881406100823 26.611964207182716 26.46190725004194 24.132247136674525 64-65 22.711085335350994 26.53030284707058 26.50110356124375 24.257508256334674 66-67 22.763036485246683 26.59825920236663 26.427036078657483 24.211668233729203 68-69 22.900172130614553 26.355543925556006 26.46811850424262 24.276165439586816 70-71 22.674389359663447 26.222546970884263 26.673436612989075 24.42962705646321 72-73 22.695267487404358 26.13672684350564 26.789727087427913 24.37827858166209 74-75 22.578172545412222 26.22727636040351 26.743588862682188 24.450962231502075 76-77 22.450812268277375 25.986782771852678 27.07040897120982 24.49199598866013 78-79 22.56195480707402 25.990725728645803 27.051821686806953 24.395497777473217 80-81 22.4963353523732 25.981409548889456 26.98581133273984 24.536443765997504 82-83 22.385942905983025 25.9845298064508 27.087632995179938 24.54189429238624 84-85 22.619323082101012 25.735326083145765 26.969803740087084 24.675547094666133 86-87 22.392533088044555 25.702284018043308 27.097878074464397 24.807304819447737 88-89 22.4976181142825 25.64600052605635 27.0876437564629 24.768737603198254 90-91 22.530017552525237 25.56682877716706 27.14106978907873 24.762083881228964 92-93 22.481282931421333 25.59410614738557 27.11188674920565 24.812724171987448 94-95 22.154259347032873 25.54510919712697 27.357950481970622 24.942680973869535 96-97 22.298530545315227 25.489628358116352 27.253352551696963 24.958488544871454 98-99 22.199077116664302 25.544870952308347 27.101469848563713 25.15458208246364 100-101 22.100425133908242 25.76789308234529 27.13524881394055 24.99643296980592 102-103 21.894353095259834 25.866796994995106 27.212498902588976 25.026351007156084 104-105 21.671827113691997 26.00784687036471 27.17320595896691 25.14712005697638 106-107 21.643631091217234 25.98792566940705 27.20026656565871 25.168176673717007 108-109 21.58079438506939 25.996574808187507 27.27900588803444 25.143624918708664 110-111 21.584505017899534 26.25496566626516 27.107489760175675 25.053039555659634 112-113 21.594152784513096 26.290316898847443 27.076285348293943 25.03924496834552 114-115 21.48734675333189 26.38428134670215 27.04312238804505 25.08524951192091 116-117 21.414002541799384 26.310042386108474 27.11441970748099 25.161535364611147 118-119 21.50330301415267 26.16355602820895 27.09689653546738 25.236244422171005 120-121 21.534295759894178 26.248561312836554 27.09097236405776 25.12617056321151 122-123 21.59721490681096 26.34408668115279 26.99140490542802 25.067293506608234 124-125 21.58672081613404 26.384541279618652 26.867328253241386 25.161409651005922 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3014.0 1 2075.0 2 983.0 3 750.0 4 575.5 5 468.0 6 461.5 7 485.0 8 530.5 9 562.5 10 584.0 11 608.5 12 658.5 13 749.5 14 870.5 15 1004.0 16 1185.5 17 1393.5 18 1692.0 19 2061.0 20 2550.0 21 3185.5 22 3907.5 23 4941.5 24 6349.5 25 8122.5 26 10816.5 27 13950.5 28 16573.5 29 19913.0 30 23768.0 31 27816.0 32 32730.5 33 38514.5 34 45142.0 35 51460.0 36 57611.5 37 63317.5 38 66962.5 39 70462.0 40 75918.0 41 81961.0 42 87827.0 43 93756.0 44 99583.0 45 104354.0 46 107993.0 47 112817.0 48 116772.5 49 119228.5 50 122347.5 51 121259.0 52 116093.5 53 112973.0 54 109772.5 55 103216.0 56 95555.5 57 86638.5 58 77517.0 59 68191.0 60 57230.0 61 46584.5 62 36916.0 63 28198.5 64 20869.5 65 15451.5 66 11505.0 67 8629.5 68 6544.5 69 5006.0 70 3903.0 71 3025.0 72 2348.5 73 1934.0 74 1620.5 75 1184.5 76 791.5 77 550.5 78 380.0 79 266.0 80 205.0 81 171.0 82 134.5 83 93.0 84 79.0 85 62.0 86 48.5 87 40.0 88 29.5 89 27.5 90 22.0 91 21.5 92 21.0 93 15.0 94 20.5 95 21.0 96 14.0 97 14.0 98 19.5 99 30.0 100 50.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2989651798028987 2 0.07169420373202129 3 0.08216639079400193 4 0.0868245877145485 5 0.09722672670253595 6 0.12874835999946765 7 0.1171554037535961 8 0.11750564412356199 9 0.12331963426499606 10-11 0.10093927462417457 12-13 0.08959148663727917 14-15 0.09995860158827004 16-17 0.10227018803004502 18-19 0.09407456337284278 20-21 0.09344413070690415 22-23 0.07412837430328434 24-25 0.0871923401030127 26-27 0.10008118571775809 28-29 0.09200814519004392 30-31 0.08696468386253486 32-33 0.09678892624007857 34-35 0.09104498417263768 36-37 0.09127264041311552 38-39 0.07983729233372865 40-41 0.09272613794847404 42-43 0.08167605427604965 44-45 0.07866398709434284 46-47 0.06792911975488779 48-49 0.0701531461041713 50-51 0.07228961236096333 52-53 0.08482821760574281 54-55 0.09037952746970246 56-57 0.09843505597891833 58-59 0.06799916782888096 60-61 0.08547616229017975 62-63 0.09100996013564108 64-65 0.08309452777441158 66-67 0.07633488863406956 68-69 0.08428534503229566 70-71 0.08326964795939454 72-73 0.06353360311181563 74-75 0.09267360189297914 76-77 0.09696404642506151 78-79 0.07829623470587864 80-81 0.09065971976567519 82-83 0.09558059696369618 84-85 0.09953831314431096 86-87 0.08720985212151099 88-89 0.06568758138710598 90-91 0.09109752022813257 92-93 0.06668576644150881 94-95 0.09547552485270641 96-97 0.08248160712697124 98-99 0.07589708817161217 100-101 0.09347915474390074 102-103 0.06568758138710598 104-105 0.07249975658294287 106-107 0.09111503224663087 108-109 0.09761199110949845 110-111 0.09085235196915643 112-113 0.07945202792676613 114-115 0.09361925089188709 116-117 0.0868070756960502 118-119 0.07906676351980364 120-121 0.11170916600062623 122-123 0.08988919095175019 124-125 0.11205940637059213 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2855182.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.255964073130755 #Duplication Level Percentage of deduplicated Percentage of total 1 68.93860843345556 33.2669901181646 2 13.927293629782458 13.441499620694502 3 6.235994797856106 9.027718227767236 4 3.445818202924044 6.651251176113707 5 2.0548850204426037 4.958022886044644 6 1.3253396907253296 3.8373326700201438 7 0.9038329672949041 3.053073183852584 8 0.6417976613729248 2.4776451911544957 9 0.44797899670238434 1.945589253334868 >10 1.9691947742556848 15.747069709477746 >50 0.07979947239514514 2.6290110027873235 >100 0.02807120055907096 2.181164143846728 >500 8.74832989725308E-4 0.2817911947204027 >1k 5.103192439592877E-4 0.501841622020985 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3772 0.13211066755114034 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 3.5024036996590764E-5 3 0.0 0.0 0.0 0.0 3.5024036996590764E-5 4 0.0 0.0 0.0 0.0 3.5024036996590764E-5 5 0.0 0.0 0.0 0.0 7.004807399318153E-5 6 1.0507211098977228E-4 0.0 0.0 0.0 1.0507211098977228E-4 7 2.1014422197954455E-4 0.0 0.0 3.5024036996590764E-5 1.0507211098977228E-4 8 2.801922959727261E-4 0.0 0.0 3.5024036996590764E-5 1.0507211098977228E-4 9 2.801922959727261E-4 0.0 0.0 7.004807399318153E-5 1.0507211098977228E-4 10-11 2.801922959727261E-4 0.0 0.0 7.004807399318153E-5 1.4009614798636305E-4 12-13 3.67752388464203E-4 0.0 0.0 7.004807399318153E-5 3.3272835146761225E-4 14-15 4.202884439590891E-4 0.0 0.0 7.004807399318153E-5 4.378004624573845E-4 16-17 4.553124809556799E-4 0.0 0.0 1.0507211098977228E-4 4.903365179522707E-4 18-19 6.129206474403383E-4 0.0 0.0 1.4009614798636305E-4 5.603845919454522E-4 20-21 7.355047769284059E-4 0.0 0.0 2.1014422197954455E-4 5.778966104437476E-4 22-23 8.055528509215875E-4 0.0 0.0 4.553124809556799E-4 6.654567029352245E-4 24-25 9.106249619113598E-4 0.0 0.0 0.0013309134058704488 6.829687214335199E-4 26-27 9.106249619113598E-4 0.0 0.0 0.00231158644177499 7.705288139249968E-4 28-29 9.981850544028366E-4 0.0 0.0 0.0060766704189084975 7.880408324232921E-4 30-31 0.0010857451468943135 0.0 0.0 0.02055910971699878 8.055528509215875E-4 32-33 0.0010857451468943135 0.0 0.0 0.058297509580825316 8.580889064164737E-4 34-35 0.001103257165392609 0.0 0.0 0.11855636523345972 9.106249619113598E-4 36-37 0.0011557932208874952 0.0 0.0 0.22338330796425587 9.106249619113598E-4 38-39 0.001330913405870449 0.0 0.0 0.3940204162116461 9.106249619113598E-4 40-41 0.001330913405870449 0.0 0.0 0.5889992301716669 9.106249619113598E-4 42-43 0.001383449461365335 0.0 0.0 0.7693379966671126 9.106249619113598E-4 44-45 0.0015060335908534026 0.0 0.0 0.990024453782631 9.106249619113598E-4 46-47 0.0017687138683278333 0.0 0.0 1.2610054280252538 9.281369804096551E-4 48-49 0.0019263220348124917 0.0 0.0 1.5404797312395497 9.806730359045413E-4 50-51 0.0019263220348124917 0.0 0.0 1.8352770506398541 0.0010857451468943135 52-53 0.0019263220348124917 0.0 0.0 2.1256788533970865 0.0011207691838909044 54-55 0.001996370108805673 0.0 0.0 2.4532936954631963 0.0011207691838909044 56-57 0.002066418182798855 0.0 0.0 2.8412724652929304 0.0011733052393857906 58-59 0.0021014422197954455 0.0 0.0 3.2716303198885397 0.0012258412948806766 60-61 0.0021364662567920365 0.0 0.0 3.7197628732599184 0.0012258412948806766 62-63 0.002206514330785218 0.0 0.0 4.167772842501809 0.0012783773503755626 64-65 0.0022765624047783994 0.0 0.0 4.639389012679402 0.0013309134058704488 66-67 0.0025217306637545346 0.0 0.0 5.19098257133871 0.0013659374428670397 68-69 0.002591778737747716 0.0 0.0 5.813709949138094 0.0013659374428670397 70-71 0.0026443147932426023 0.0 0.0 6.496100773961169 0.001418473498361926 72-73 0.0027493869042323747 0.0 0.0 7.172309856254348 0.001523545609351698 74-75 0.002801922959727261 0.0 0.0 7.839832977372371 0.0015935936833448797 76-77 0.002801922959727261 0.0 0.0 8.589469953228901 0.001611105701843175 78-79 0.002801922959727261 0.0 0.0 9.430099377202573 0.001611105701843175 80-81 0.003047091218703396 0.0 0.0 10.312389893183692 0.001611105701843175 82-83 0.003047091218703396 0.0 0.0 11.220247956172322 0.0016461297388397657 84-85 0.0031696753481914638 0.0 0.0 12.118194216690915 0.0016461297388397657 86-87 0.0032922594776795314 0.0 0.0 13.06389575165436 0.0017161778128329474 88-89 0.0032922594776795314 1.7512018498295382E-5 0.0 14.092691814392218 0.0017862258868261288 90-91 0.0034323556256658943 5.2536055494886145E-5 0.0 15.176423079159227 0.0017862258868261288 92-93 0.0034323556256658943 8.75600924914769E-5 0.0 16.287543140857572 0.0018562739608193103 94-95 0.0034323556256658943 1.2258412948806767E-4 0.0 17.395318407022742 0.001891297997815901 96-97 0.0034323556256658943 1.5760816648465842E-4 0.0 18.49843547626736 0.0019263220348124917 98-99 0.0035199157181573714 1.751201849829538E-4 0.0 19.65421118513636 0.0019263220348124917 100-101 0.003607475810648848 1.751201849829538E-4 0.0 20.9040439453597 0.0019263220348124917 102-103 0.003730059940136916 1.751201849829538E-4 0.0 22.167290911752737 0.0019963701088056736 104-105 0.004027764254607938 1.751201849829538E-4 0.0 23.37754651016993 0.002031394145802264 106-107 0.004027764254607938 1.751201849829538E-4 0.0 24.588187372994085 0.002031394145802264 108-109 0.004027764254607938 1.751201849829538E-4 0.0 25.82024543444166 0.002031394145802264 110-111 0.004027764254607938 1.751201849829538E-4 0.0 27.08168866292937 0.002066418182798855 112-113 0.004027764254607938 1.751201849829538E-4 0.0 28.383654702222135 0.0021014422197954455 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 12435 0.0 27.488247 1 TACATGG 12265 0.0 27.080175 2 CATGGGG 6640 0.0 26.984459 4 TAGTACG 155 6.811759E-6 26.88324 4 GTATCAA 8435 0.0 26.308498 1 GGTATCA 6695 0.0 26.196 1 ACATGGG 12920 0.0 24.603338 3 AACGCAG 9415 0.0 22.883356 6 TCAACGC 9595 0.0 22.396362 4 TATCAAC 9785 0.0 22.264095 2 CAACGCA 9815 0.0 22.136566 5 ATCAACG 9925 0.0 21.651697 3 ACGCAGA 10385 0.0 20.45726 7 GTATAAT 1230 0.0 19.884604 1 GTATAGA 875 0.0 19.770979 1 CGCAGAG 10720 0.0 19.540752 8 GAGTACT 3665 0.0 18.74972 12-13 ATGGGGA 3735 0.0 18.009245 5 TATACCG 200 0.001171457 17.85784 5 GCAGAGT 11620 0.0 17.105421 9 >>END_MODULE