FastQCFastQC Report
Mon 15 Aug 2016
SRR2921069_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921069_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2855182
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73480.2573566238509489No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65060.22786638469981949No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT54630.19133631411237534No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46120.16153085862827657No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT38060.13330148480902443RNA PCR Primer, Index 33 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83950.038.6607551
GTATCAA118650.036.405161
TCAACGC147300.028.887824
TATCAAC149000.028.8787162
CAACGCA147450.028.7781035
ATCAACG149050.028.7085843
AACGCAG150850.028.2537866
ACGCAGA167200.025.4739657
GAGTACT93950.025.10512512-13
CGCAGAG172100.024.7832418
CATGGGG70450.023.9400334
AGTACTT100650.023.0497812-13
GCAGAGT182600.022.8039179
GTACATG134300.022.6561571
GTACTTT103300.022.57562414-15
TACATGG133400.022.1591342
AGAGTAC169200.020.95302210-11
ACATGGG136300.020.9439683
CAGAGTA175800.020.55550610-11
CCCTACG4650.020.528591
Produced by FastQC (version 0.11.5)