FastQCFastQC Report
Mon 15 Aug 2016
SRR2921068_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921068_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7707101
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA150000.037.619891
GGTATCA105350.034.9922261
GTACATG283350.034.335891
TACATGG289600.032.5949752
TCAACGC168150.032.0690274
ACATGGG288950.031.7420083
CATGGGG206500.030.955564
ATCAACG176100.030.6550753
CAACGCA176400.030.4001035
AACGCAG178350.030.0304476
TATCAAC184350.029.6054862
ACGCAGA203950.026.0548827
CGCAGAG216950.024.4389328
ATGGGGA117900.022.1110365
ATGGGGG108050.020.3810125
GCAGAGT249900.020.1925419
AGAGTAC215300.018.59902210-11
CAGAGTA237750.017.51848410-11
ATGGGGC78450.016.5391185
GTATATA36600.016.1351031
Produced by FastQC (version 0.11.5)