FastQCFastQC Report
Mon 15 Aug 2016
SRR2921068_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921068_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7707101
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106650.13837887942561022No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94510.12262717200669875No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA124800.049.912251
GTATCAA193650.043.751411
TCAACGC239400.034.7080654
CAACGCA245800.033.7801065
ATCAACG246800.033.739843
AACGCAG252350.032.9530146
TATCAAC254800.032.751112
GTACATG274250.028.8699151
ACGCAGA289150.028.452237
TACATGG280150.027.9151942
ACATGGG277150.026.9695993
CGCAGAG304200.026.9668888
CATGGGG196550.026.5657964
GCAGAGT341700.023.57149
AGAGTAC306950.022.80050510-11
GAGTACT181350.022.06024712-13
CAGAGTA325600.021.30268310-11
AGTACTT198500.020.87354712-13
GTACTTT201200.019.85541714-15
ATGGGGA110400.018.7349365
Produced by FastQC (version 0.11.5)