##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2921068_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7707101 Sequences flagged as poor quality 0 Sequence length 125 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.315541083476134 33.0 31.0 34.0 28.0 34.0 2 31.407695059400414 33.0 31.0 34.0 28.0 34.0 3 31.51573749974212 34.0 31.0 34.0 28.0 34.0 4 34.99271645719966 37.0 35.0 37.0 32.0 37.0 5 34.73550495834945 37.0 35.0 37.0 32.0 37.0 6 34.49385041145821 37.0 35.0 37.0 31.0 37.0 7 34.445107570278374 37.0 35.0 37.0 30.0 37.0 8 34.43808910769432 37.0 35.0 37.0 30.0 37.0 9 36.00273812423115 39.0 37.0 39.0 30.0 39.0 10-11 35.96698564090441 39.0 37.0 39.0 30.0 39.0 12-13 35.905201839187 39.0 37.0 39.0 30.0 39.0 14-15 37.19416042426329 40.0 37.5 41.0 31.0 41.0 16-17 37.17847398133228 40.0 37.5 41.0 31.0 41.0 18-19 37.18707377780569 40.0 37.5 41.0 31.0 41.0 20-21 37.18916580955667 40.0 38.0 41.0 31.0 41.0 22-23 37.149090494597125 40.0 37.5 41.0 31.0 41.0 24-25 37.11089798875089 40.0 37.0 41.0 30.5 41.0 26-27 37.00568261658956 40.0 37.0 41.0 30.0 41.0 28-29 36.89314030009468 40.0 37.0 41.0 30.0 41.0 30-31 36.779689405912805 40.0 37.0 41.0 30.0 41.0 32-33 36.682419109338255 40.0 37.0 41.0 30.0 41.0 34-35 36.55538522201798 40.0 36.0 41.0 30.0 41.0 36-37 36.39529986696684 40.0 36.0 41.0 29.5 41.0 38-39 36.271455116521764 40.0 36.0 41.0 29.0 41.0 40-41 36.15186585721402 40.0 36.0 41.0 28.0 41.0 42-43 36.0147548994103 40.0 35.0 41.0 28.0 41.0 44-45 35.86348965713567 40.0 35.0 41.0 27.0 41.0 46-47 35.63851615542602 40.0 35.0 41.0 26.5 41.0 48-49 35.42135616232355 40.0 35.0 41.0 26.0 41.0 50-51 35.243843502245525 39.0 35.0 41.0 25.5 41.0 52-53 35.04465323602221 39.0 35.0 41.0 24.0 41.0 54-55 34.84301211311491 39.0 35.0 41.0 24.0 41.0 56-57 34.60331997725214 39.0 34.0 41.0 23.5 41.0 58-59 34.360323291468475 38.0 34.0 40.5 23.0 41.0 60-61 34.078859807338716 38.0 34.0 40.0 22.5 41.0 62-63 33.79357880479314 37.0 34.0 40.0 22.0 41.0 64-65 33.48263452107349 37.0 33.5 40.0 21.5 41.0 66-67 33.15388003868121 36.0 33.0 39.0 20.5 41.0 68-69 32.80700213997455 36.0 33.0 39.0 20.0 41.0 70-71 32.431057799813445 35.0 32.5 38.5 19.0 40.5 72-73 32.06021959489048 35.0 32.0 37.5 18.0 40.0 74-75 31.73584243932965 35.0 32.0 37.0 17.5 39.0 76-77 31.434464074105165 35.0 32.0 36.5 16.0 39.0 78-79 31.137649422266556 35.0 32.0 36.0 14.0 38.5 80-81 31.095252092842692 35.0 32.0 36.0 14.5 37.0 82-83 30.896097832375624 35.0 32.0 35.0 12.5 37.0 84-85 30.6959932794445 35.0 32.0 35.0 10.5 36.5 86-87 30.52694184233475 35.0 32.0 35.0 7.5 36.0 88-89 30.353811374730913 35.0 32.0 35.0 7.0 36.0 90-91 30.20081175788406 35.0 31.5 35.0 7.0 36.0 92-93 30.097053937660867 35.0 31.0 35.0 3.5 35.0 94-95 29.996227829374497 35.0 31.0 35.0 2.0 35.0 96-97 29.928919966145507 35.0 31.0 35.0 2.0 35.0 98-99 29.83874105192082 35.0 31.0 35.0 2.0 35.0 100-101 29.749221270098836 35.0 31.0 35.0 2.0 35.0 102-103 28.42954198991294 33.0 28.0 34.5 2.0 35.0 104-105 29.442588918972255 34.0 30.5 35.0 2.0 35.0 106-107 29.608156296381736 35.0 31.0 35.0 2.0 35.0 108-109 29.6092973869163 35.0 31.0 35.0 2.0 35.0 110-111 29.52264191166043 35.0 31.0 35.0 2.0 35.0 112-113 29.417498421261122 35.0 31.0 35.0 2.0 35.0 114-115 29.336339500416564 35.0 31.0 35.0 2.0 35.0 116-117 29.233465086288604 35.0 31.0 35.0 2.0 35.0 118-119 29.090217709616105 34.5 30.5 35.0 2.0 35.0 120-121 29.221442926464825 35.0 31.0 35.0 2.0 35.0 122-123 29.136918927103718 35.0 31.0 35.0 2.0 35.0 124-125 28.476985769357377 34.5 30.0 35.0 2.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 140111.0 3 51292.0 4 22695.0 5 19404.0 6 21403.0 7 24558.0 8 27669.0 9 30719.0 10 34590.0 11 38470.0 12 41572.0 13 46997.0 14 56937.0 15 52111.0 16 33415.0 17 29346.0 18 27520.0 19 26848.0 20 27457.0 21 28122.0 22 29789.0 23 34618.0 24 44630.0 25 59143.0 26 73068.0 27 86663.0 28 104280.0 29 126211.0 30 151613.0 31 185201.0 32 233779.0 33 301734.0 34 417169.0 35 640149.0 36 1249177.0 37 2287288.0 38 899857.0 39 1496.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.59931199842154 18.363748924916298 12.470529026317632 25.56641005034453 2 16.389674729934324 20.176440184833446 37.6876691580434 25.746215927188835 3 18.7725319805722 24.14837952688047 29.845696845026424 27.233391647520904 4 13.118851610818766 16.29812392829964 36.077292830547584 34.505731630334004 5 13.762273741317408 37.48248279009247 34.54338570294217 14.211857765647961 6 33.3408732847963 36.178743076240465 16.65339384166024 13.826989797302991 7 28.453661917313422 32.10251886616052 21.15031642674654 18.293502789779513 8 26.86633906710427 34.78925160611854 19.498839628992716 18.845569697784473 9 26.6704203595318 14.789709933145941 19.013782298876915 39.52608740844534 10-11 25.533354500949233 25.361556850035573 27.901755099853492 21.2033335491617 12-13 26.114421976495912 22.96655470503878 27.76428639693147 23.154736921533843 14-15 23.26620224219579 24.344529605583944 25.119291306831133 27.269976845389138 16-17 23.272024298593276 27.190796007843538 25.92172643501092 23.61545325855226 18-19 23.344445567722573 26.28285896749918 27.58056099566797 22.792134469110277 20-21 24.170291478969492 25.486131925496096 27.35852135820327 22.985055237331142 22-23 23.750508183333405 25.369731967968146 27.293064339822397 23.58669550887605 24-25 23.45110930005724 25.661370837419618 27.374724559277 23.512795303246143 26-27 23.35891395327484 25.71955105379328 27.47306669919806 23.448468293733825 28-29 23.4937701350626 25.83951466128451 27.389784703426034 23.276930500226854 30-31 23.667416333461 25.76838034465292 27.116612532236346 23.447590789649745 32-33 23.363593883725848 25.851820574461833 27.15370624418051 23.630879297631814 34-35 23.578594514733762 25.80730188690999 27.273832179840984 23.340271418515265 36-37 23.755098210946745 25.600119606350276 27.14466508761885 23.50011709508413 38-39 23.584879215617878 25.830094144127308 27.109515481663284 23.475511158591534 40-41 23.688197805179986 25.725985391617094 27.209711130808028 23.37610567239489 42-43 23.60904869770111 25.704385017521535 27.0252034489176 23.66136283585975 44-45 23.61051470638338 25.679418461315823 27.028109022603964 23.681957809696833 46-47 23.743396397828704 25.716803535332232 26.76380177999565 23.77599828684341 48-49 23.622657949129298 25.917292578357326 26.617515128100745 23.84253434441263 50-51 23.604607803424198 26.112025872777682 26.518208601865467 23.765157721932653 52-53 23.794629277615883 25.964601537932875 26.371943781462793 23.868825402988453 54-55 23.595084292282113 25.922769070928492 26.479353656433307 24.00279298035609 56-57 23.512466206496686 26.057005242543045 26.419392023844303 24.011136527115973 58-59 23.473614737034982 25.93221821413268 26.531141246615338 24.063025802217002 60-61 23.48101406682881 25.97593884676959 26.61628572209057 23.92676136431103 62-63 23.393510982593472 25.96353784286885 26.626022524427267 24.016928650110412 64-65 23.21560256111087 26.02089604617413 26.56735920418362 24.19614218853138 66-67 23.257921910949015 26.095503975725627 26.46507408370395 24.18150002962141 68-69 23.37867018631755 25.830560111533185 26.547673189748068 24.243096512401195 70-71 23.174767649091077 25.78196340216863 26.61357537731877 24.429693571421527 72-73 23.30783236632045 25.82613911526287 26.481566770483706 24.384461747932978 74-75 23.138180146254907 25.96330247846412 26.37333453069034 24.52518284459063 76-77 23.22240144356335 25.80382458385213 26.42008474550429 24.55368922708023 78-79 23.13712495583021 26.008294252613428 26.331877292919764 24.52270349863659 80-81 23.151144429529367 25.954950058708658 26.28560153275798 24.608303979004 82-83 23.11940979025462 25.87991066889516 26.412415567552223 24.588263973297998 84-85 22.98463453648646 25.948306229075197 26.226225472814065 24.84083376162428 86-87 22.86570830847296 25.91916769157583 26.362949682950763 24.852174317000447 88-89 22.920584859557604 25.87631470890693 26.19381486593746 25.00928556559801 90-91 22.753852173354453 25.94784520320308 26.240465680342446 25.057836943100025 92-93 22.888918635160042 25.891398094281996 26.236762684039643 24.98292058651832 94-95 22.699401071725408 25.81472996321824 26.319767098587967 25.166101866468388 96-97 22.73458007346303 25.698965876165087 26.303935684452235 25.262518365919657 98-99 22.649397669792435 25.812273665456782 26.162203221425784 25.376125443324998 100-101 22.571826349780665 25.846246374240305 26.18081108255655 25.401116193422478 102-103 22.432167197624988 25.830743862486898 26.19321570750694 25.54387323238117 104-105 22.339234498904858 25.972905926900676 25.957041822774535 25.73081775141993 106-107 22.34940863293714 25.996674523442987 25.938094226161606 25.71582261745827 108-109 22.216311504553392 26.080701085080598 25.929124590921244 25.77386281944477 110-111 22.219347565931834 26.219551841733235 25.803446965965755 25.757653626369176 112-113 22.166787517782957 26.376442305352647 25.72510507558467 25.73166510127972 114-115 22.136557774158426 26.557898735041235 25.55097577880176 25.754567711998583 116-117 21.99355784524879 26.458900849924525 25.68817803938285 25.85936326544383 118-119 22.01529301071676 26.475311122636697 25.53528976152293 25.974106105123617 120-121 21.888631821753926 26.657578596270714 25.505123295881376 25.948666286093985 122-123 21.769008707759195 26.74356795915726 25.43448127311118 26.052942059972366 124-125 21.75366625457633 26.88635129818836 25.301581098236316 26.058401348998995 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 106.0 1 126.5 2 303.0 3 524.0 4 447.5 5 343.0 6 536.0 7 1159.0 8 1870.5 9 2063.0 10 1737.0 11 1514.5 12 1654.5 13 1898.0 14 2227.5 15 2628.5 16 3052.5 17 3437.0 18 3910.5 19 4472.5 20 5100.5 21 6024.5 22 7446.0 23 9382.0 24 11888.0 25 15560.5 26 21377.5 27 28891.5 28 37208.5 29 47333.5 30 58845.5 31 72285.0 32 87738.0 33 99219.0 34 110601.5 35 124326.0 36 139546.5 37 155018.0 38 169321.5 39 183659.5 40 196651.0 41 210086.0 42 224003.0 43 242095.0 44 260951.0 45 277006.5 46 293627.0 47 304582.5 48 312294.0 49 318218.5 50 322219.5 51 324344.5 52 320208.0 53 316237.5 54 311556.5 55 297443.5 56 276223.0 57 252788.5 58 232770.0 59 209179.5 60 175175.0 61 142902.5 62 114757.5 63 86706.0 64 63341.5 65 47924.0 66 36678.0 67 28424.0 68 21373.0 69 15517.0 70 11854.0 71 8963.5 72 6679.0 73 5457.0 74 4336.5 75 3233.5 76 2272.0 77 1497.0 78 968.5 79 536.5 80 353.0 81 236.0 82 155.5 83 105.0 84 61.0 85 45.0 86 41.5 87 38.0 88 29.0 89 21.5 90 18.5 91 15.0 92 15.5 93 15.5 94 13.5 95 25.0 96 37.5 97 34.5 98 19.0 99 6.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17534478917559274 2 0.011937043513507868 3 0.0 4 0.003970364472971095 5 0.1439970749053373 6 0.07657872914861243 7 0.00749957733783429 8 0.012261419695940145 9 0.011833243135129538 10-11 0.004671017027024818 12-13 0.009608022523644105 14-15 0.011165028199319044 16-17 0.014798041442560568 18-19 0.01920306999999092 20-21 0.022829595719583796 22-23 0.025411630131744738 24-25 0.02529485470606912 26-27 0.022362494016881316 28-29 0.02124015242566563 30-31 0.024652589864853205 32-33 0.024062225212826457 34-35 0.023419960371610545 36-37 0.023672973793907724 38-39 0.019060344479720714 40-41 0.02600848230742013 42-43 0.022407906682421836 44-45 0.03073139952363411 46-47 0.026287445824311893 48-49 0.018268866594585955 50-51 0.014097388888506847 52-53 0.015219730479722532 54-55 0.01756821404053223 56-57 0.014376352405398606 58-59 0.013221573195939694 60-61 0.01657562292228946 62-63 0.014421765070939124 64-65 0.026040919925663358 66-67 0.019806409699314957 68-69 0.01832725430742376 70-71 0.025236466993231305 72-73 0.030627599145255785 74-75 0.01608905864864104 76-77 0.00798614161148271 78-79 0.01351999928377739 80-81 0.016257734263505824 82-83 0.015083492483100973 84-85 0.014084413841209556 86-87 0.009432859385130674 88-89 0.02066925034458482 90-91 0.017983415554045548 92-93 0.015394893618235964 94-95 0.01084713954053541 96-97 0.020662762820936174 98-99 0.021966755074313934 100-101 0.02807800235133807 102-103 0.019942647695936514 104-105 0.013292935956074793 106-107 0.018664605537153333 108-109 0.009874010993238572 110-111 0.023770286648637406 112-113 0.017367100807424218 114-115 0.01297504729729116 116-117 0.010749826685805727 118-119 0.0074476771486451255 120-121 0.0038211514290522465 122-123 0.006078809658780909 124-125 6.876775067564315E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 7707101.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.194241165485096 #Duplication Level Percentage of deduplicated Percentage of total 1 65.11334278606974 25.520680602481168 2 14.7295194762303 11.546246772061602 3 6.7139678354612125 7.894466235611302 4 3.857501275858788 6.047673412087031 5 2.297677514478062 4.502786331148277 6 1.5252213791301532 3.58679367386286 7 1.0991244988280302 3.0155545474571133 8 0.7949713438512991 2.4926638856446073 9 0.595355855494704 2.100106888158881 >10 3.010365047415385 20.62080009361676 >50 0.17333632304144 4.669703937908423 >100 0.08418410256002186 5.775898300820899 >500 0.003776493721994297 1.000312393967789 >1k 0.001556703791402625 0.8868164464742756 >5k 6.624271159077157E-5 0.2010470314168087 >10k+ 3.3121355795385785E-5 0.13844944728225386 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10665 0.13837887942561022 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9451 0.12262717200669875 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 7.785028378374696E-5 2 0.0 0.0 0.0 0.0 7.785028378374696E-5 3 0.0 0.0 0.0 0.0 7.785028378374696E-5 4 0.0 0.0 0.0 0.0 7.785028378374696E-5 5 0.0 0.0 0.0 0.0 7.785028378374696E-5 6 0.0 0.0 0.0 0.0 1.297504729729116E-4 7 0.0 0.0 0.0 0.0 1.297504729729116E-4 8 0.0 0.0 0.0 0.0 1.297504729729116E-4 9 0.0 0.0 0.0 0.0 1.297504729729116E-4 10-11 0.0 0.0 0.0 6.48752364864558E-6 1.5570056756749392E-4 12-13 0.0 0.0 0.0 1.297504729729116E-5 3.957389425673804E-4 14-15 0.0 0.0 0.0 1.297504729729116E-5 5.190018918916464E-4 16-17 0.0 0.0 0.0 2.595009459458232E-5 5.903646520267478E-4 18-19 0.0 0.0 0.0 3.24376182432279E-5 6.228022702699757E-4 20-21 0.0 0.0 0.0 1.1677542567562045E-4 7.201151249996594E-4 22-23 0.0 0.0 0.0 2.0111323310801298E-4 7.590402668915329E-4 24-25 0.0 1.297504729729116E-5 0.0 3.8925141891873484E-4 7.655277905401785E-4 26-27 0.0 1.297504729729116E-5 0.0 8.758156925671533E-4 8.174279797293431E-4 28-29 0.0 1.297504729729116E-5 0.0 0.00302318602026884 8.693281689185078E-4 30-31 0.0 1.297504729729116E-5 0.0 0.011755392851345792 9.017657871617357E-4 32-33 0.0 1.297504729729116E-5 0.0 0.03371566040201108 9.342034054049635E-4 34-35 0.0 1.297504729729116E-5 0.0 0.0659002652229418 0.0010120536891887106 36-37 0.0 1.297504729729116E-5 0.0 0.1221211451621044 0.0010120536891887106 38-39 0.0 1.297504729729116E-5 0.0 0.2137120040336827 0.0011028790202697487 40-41 0.0 1.946257094593674E-5 0.0 0.3182454725843089 0.0011223415912156854 42-43 0.0 3.892514189187348E-5 0.0 0.42242212733425966 0.001141804162161622 44-45 0.0 3.892514189187348E-5 0.0 0.5502522933071722 0.0011872168277021413 46-47 0.0 4.541266554051906E-5 0.0 0.7069843771348008 0.001252092064188597 48-49 0.0 5.190018918916464E-5 0.0 0.8760687059894505 0.001297504729729116 50-51 0.0 5.190018918916464E-5 0.0 1.0590428229758504 0.0013364298716209894 52-53 0.0 5.190018918916464E-5 0.0 1.244242160573736 0.0014013051081074453 54-55 0.0 5.190018918916464E-5 0.0 1.4479504031412072 0.0014337427263506732 56-57 0.0 5.190018918916464E-5 0.0 1.6924781445059562 0.0014726678682425469 58-59 0.0 5.190018918916464E-5 0.0 1.9630013931308286 0.0014921304391884835 60-61 0.0 5.190018918916464E-5 0.0 2.253389439167853 0.0014921304391884835 62-63 0.0 5.190018918916464E-5 0.0 2.5511408245460907 0.0015959308175668128 64-65 0.0 5.190018918916464E-5 0.0 2.865532708083104 0.0016283684358100407 66-67 0.0 5.190018918916464E-5 0.0 3.233109310491714 0.0016478310067559775 68-69 0.0 5.190018918916464E-5 0.0 3.6501078680557058 0.0016608060540532685 70-71 0.0 5.838771283781022E-5 0.0 4.100854523639953 0.0016867561486478507 72-73 0.0 6.48752364864558E-5 0.0 4.561066476227572 0.0018748943344585726 74-75 0.0 6.48752364864558E-5 0.0 5.032287756446944 0.0019851822364855476 76-77 0.0 7.785028378374696E-5 0.0 5.552191414125752 0.0020630325202692945 78-79 0.0 7.785028378374696E-5 0.0 6.136341018497097 0.002088982614863877 80-81 0.0 7.785028378374696E-5 0.0 6.761874536223154 0.002101957662161168 82-83 0.0 7.785028378374696E-5 0.0 7.409939223580955 0.0022317081351340795 84-85 0.0 7.785028378374696E-5 0.0 8.054293047411731 0.0022446831824313707 86-87 0.0 7.785028378374696E-5 0.0 8.732894508583708 0.002283608324323244 88-89 0.0 7.785028378374696E-5 0.0 9.466821571431332 0.002335508513512409 90-91 0.0 7.785028378374696E-5 0.0 10.257248477735015 0.0023549710844583457 92-93 0.0 7.785028378374696E-5 0.0 11.068785785991388 0.0024133587972961557 94-95 0.0 7.785028378374696E-5 0.0 11.874276722207222 0.0024133587972961557 96-97 0.0 7.785028378374696E-5 0.0 12.692333991730482 0.002601496983106878 98-99 0.0 7.785028378374696E-5 0.0 13.560929070476694 0.0027442225033770806 100-101 0.0 7.785028378374696E-5 0.0 14.491317811976254 0.002750710027025726 102-103 0.0 7.785028378374696E-5 0.0 15.444873500425128 0.0028285603108094727 104-105 6.48752364864558E-6 7.785028378374696E-5 0.0 16.380303047799686 0.0028804604999986376 106-107 1.297504729729116E-5 9.082533108103813E-5 0.0 17.31450645320465 0.0028804604999986376 108-109 1.297504729729116E-5 9.082533108103813E-5 0.0 18.2652984565792 0.002925873165539157 110-111 1.297504729729116E-5 9.082533108103813E-5 0.0 19.266174142521294 0.0029712858310796757 112-113 1.297504729729116E-5 9.082533108103813E-5 0.0 20.28945773514581 0.0029712858310796757 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 12480 0.0 49.91225 1 GTATCAA 19365 0.0 43.75141 1 TCAACGC 23940 0.0 34.708065 4 CAACGCA 24580 0.0 33.780106 5 ATCAACG 24680 0.0 33.73984 3 AACGCAG 25235 0.0 32.953014 6 TATCAAC 25480 0.0 32.75111 2 GTACATG 27425 0.0 28.869915 1 ACGCAGA 28915 0.0 28.45223 7 TACATGG 28015 0.0 27.915194 2 ACATGGG 27715 0.0 26.969599 3 CGCAGAG 30420 0.0 26.966888 8 CATGGGG 19655 0.0 26.565796 4 GCAGAGT 34170 0.0 23.5714 9 AGAGTAC 30695 0.0 22.800505 10-11 GAGTACT 18135 0.0 22.060247 12-13 CAGAGTA 32560 0.0 21.302683 10-11 AGTACTT 19850 0.0 20.873547 12-13 GTACTTT 20120 0.0 19.855417 14-15 ATGGGGA 11040 0.0 18.734936 5 >>END_MODULE