FastQCFastQC Report
Mon 15 Aug 2016
SRR2921011_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921011_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2306835
Sequences flagged as poor quality0
Sequence length125
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG103000.027.1609151
CATGGGG63500.026.8017444
TACATGG106150.025.5654412
GCGTTAG1651.1071603E-525.252121
ACATGGG107650.024.876973
GTATCAA64650.024.2142491
GTATCGC1852.6925829E-522.5128866
TCAACGC69550.021.390144
CAACGCA70150.021.1209875
ATCAACG72650.020.5606613
AACGCAG71800.020.5509596
TATCAAC73900.020.5354462
GGTATCA51350.019.4738851
AACGCGG1908.32672E-418.788956
GTATATA12100.018.693131
GTATAAG9650.018.5045151
ATGGGGG32850.018.2948365
CGCAGAG82600.017.575788
ACGCAGA82350.017.5568877
ATGGGGA36950.017.553135
Produced by FastQC (version 0.11.5)