##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921011_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2306835
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.460221472276952	31.0	28.0	33.0	16.0	34.0
2	28.463711535502107	31.0	28.0	34.0	16.0	34.0
3	28.56013238918258	31.0	28.0	34.0	16.0	34.0
4	31.995941192152884	35.0	32.0	37.0	19.0	37.0
5	32.08546384982021	35.0	32.0	37.0	20.0	37.0
6	32.05014272802346	35.0	32.0	37.0	19.0	37.0
7	31.859746362440312	35.0	32.0	37.0	18.0	37.0
8	31.82889716863148	35.0	32.0	37.0	18.0	37.0
9	32.92010785340087	37.0	32.0	39.0	17.0	39.0
10-11	32.90776843597396	37.0	32.0	39.0	17.0	39.0
12-13	32.85801325192309	37.0	32.0	39.0	17.0	39.0
14-15	33.76720311595758	38.0	32.5	40.5	17.0	41.0
16-17	33.67564997063076	38.0	32.0	40.0	16.5	41.0
18-19	33.7126751588215	38.0	32.0	40.0	17.0	41.0
20-21	33.73900582399695	38.0	32.0	40.0	17.0	41.0
22-23	33.66644515104028	38.0	32.0	40.0	16.5	41.0
24-25	33.58989849729174	38.0	32.0	40.0	16.0	41.0
26-27	33.445752513725516	38.0	32.0	40.0	15.0	41.0
28-29	33.383873575700036	38.0	32.0	40.0	14.5	41.0
30-31	33.243916448293874	38.0	32.0	40.0	10.0	41.0
32-33	33.16640917100703	38.0	32.0	40.0	9.5	41.0
34-35	33.00898872264379	38.0	31.0	40.0	9.0	41.0
36-37	32.843825197727625	38.0	31.0	40.0	8.5	41.0
38-39	32.698044073373254	38.0	31.0	40.0	8.0	41.0
40-41	32.609317311381176	38.0	31.0	40.0	7.5	41.0
42-43	32.39022426831568	38.0	31.0	40.0	3.5	41.0
44-45	32.21672681401141	38.0	30.5	40.0	2.0	41.0
46-47	32.01693424107056	37.0	30.0	40.0	2.0	41.0
48-49	31.840821515192893	37.0	30.0	40.0	2.0	41.0
50-51	31.592652053571236	37.0	30.0	40.0	2.0	41.0
52-53	31.398749368723813	37.0	30.0	40.0	2.0	41.0
54-55	31.18981223191082	36.0	29.5	40.0	2.0	41.0
56-57	30.974793602489992	36.0	29.0	40.0	2.0	41.0
58-59	30.710016321063275	35.5	29.0	39.0	2.0	41.0
60-61	30.52383698877466	35.0	28.5	39.0	2.0	41.0
62-63	30.415033151482444	35.0	29.0	39.0	2.0	41.0
64-65	30.189463268937743	35.0	29.0	39.0	2.0	40.5
66-67	29.905320059735523	35.0	28.0	38.5	2.0	40.0
68-69	29.59307817854333	35.0	28.0	38.0	2.0	40.0
70-71	29.26431084147761	34.0	28.0	37.0	2.0	40.0
72-73	28.981040473202462	34.0	28.0	37.0	2.0	39.0
74-75	28.68321899919153	34.0	27.5	36.0	2.0	39.0
76-77	28.402307707313266	34.0	27.0	36.0	2.0	38.5
78-79	28.093590785643535	34.0	26.5	35.0	2.0	37.5
80-81	27.795743301970013	34.0	26.0	35.0	2.0	37.0
82-83	27.530987478514934	33.5	26.0	35.0	2.0	36.5
84-85	27.285176009554213	33.0	26.0	35.0	2.0	36.0
86-87	27.210028458905818	33.0	26.0	35.0	2.0	36.0
88-89	27.30795700602774	34.0	26.0	35.0	2.0	35.5
90-91	27.221468808995873	34.0	26.0	35.0	2.0	35.0
92-93	27.06715694880648	34.0	26.0	35.0	2.0	35.0
94-95	26.97293781306422	34.0	25.5	35.0	2.0	35.0
96-97	26.873512193113072	34.0	25.0	35.0	2.0	35.0
98-99	26.807048401814605	33.5	25.0	35.0	2.0	35.0
100-101	26.6336677742448	33.0	25.0	35.0	2.0	35.0
102-103	25.292393690922843	31.5	22.0	34.0	2.0	35.0
104-105	26.33139973166698	33.0	24.5	35.0	2.0	35.0
106-107	26.51380311118914	33.0	25.0	35.0	2.0	35.0
108-109	26.505371862313517	33.5	25.0	35.0	2.0	35.0
110-111	26.399126075336987	33.0	25.0	35.0	2.0	35.0
112-113	26.304649660682276	33.0	24.0	35.0	2.0	35.0
114-115	26.182675397243408	33.0	24.0	35.0	2.0	35.0
116-117	26.041635834379136	33.0	24.0	35.0	2.0	35.0
118-119	25.833946511128886	33.0	23.0	35.0	2.0	35.0
120-121	25.611475463134553	33.0	20.0	35.0	2.0	35.0
122-123	25.374617387025946	33.0	18.5	35.0	2.0	35.0
124-125	24.199302074053843	31.5	2.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	133962.0
3	20017.0
4	12811.0
5	11465.0
6	12833.0
7	14215.0
8	14697.0
9	15953.0
10	16441.0
11	16776.0
12	16477.0
13	16980.0
14	16836.0
15	15459.0
16	13446.0
17	12125.0
18	11355.0
19	11055.0
20	11226.0
21	12181.0
22	14530.0
23	19456.0
24	25718.0
25	33385.0
26	38935.0
27	44063.0
28	51295.0
29	59685.0
30	69761.0
31	83654.0
32	101328.0
33	126863.0
34	163862.0
35	223656.0
36	340212.0
37	397448.0
38	106555.0
39	119.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.797876635313	18.689737504320146	12.307761690192393	25.204624170174455
2	16.08378449741398	19.850020009182977	38.69424797772846	25.37194751567458
3	19.497496457578528	23.932330644503914	28.613876926652367	27.956295971265195
4	12.988986622742077	15.504273585801453	35.67010076843465	35.836639023021824
5	14.19374586230353	37.57910668677763	33.895922762132145	14.331224688786696
6	34.205352633506955	35.32243905293892	16.588525240439324	13.883683073114797
7	29.445882266238353	31.72980799660125	20.51267378496833	18.311635952192066
8	26.768532202483115	35.682604724236036	19.18631846953723	18.362544603743615
9	26.644661833926804	16.08104159676361	18.717416504318166	38.556880064991425
10-11	25.65934387497426	26.133500959152045	27.051967616425888	21.15518754944781
12-13	25.87211060357738	23.39351412208454	27.242126537675116	23.49224873666297
14-15	23.110818062624375	24.90348582475484	24.272651271650528	27.71304484097026
16-17	23.573581351797444	27.892605288454764	25.182117787818065	23.351695571929724
18-19	23.7026756795947	27.131768663941862	26.425996316597484	22.73955933986595
20-21	24.29924246939933	26.302041619640264	26.205239660711953	23.193476250248455
22-23	23.97833115283677	25.96440371872201	26.110969501659	23.94629562678222
24-25	23.652311728531533	26.32308711367512	26.207082359040783	23.81751879875256
26-27	23.57813184452766	26.416828740746713	26.132193927082596	23.87284548764303
28-29	23.682677866638578	26.523506784206546	26.216001749616296	23.57781359953858
30-31	23.80781664703275	26.410699635752007	26.066067498046543	23.7154162191687
32-33	23.514642356302033	26.473826693283222	26.100718083434664	23.910812866980084
34-35	23.68381807856211	26.472703547476712	26.168634115255063	23.674844258706123
36-37	23.912867714238462	26.240457415454422	26.119440351896728	23.727234518410395
38-39	23.84983708052815	26.4167968425723	26.01949571584538	23.713870361054173
40-41	23.777497593544002	26.51471259838509	26.040422391821107	23.667367416249803
42-43	23.870089132247006	26.345707923150584	26.046418475487876	23.737784469114533
44-45	23.712759800850957	26.419933978234063	26.082455680234435	23.784850540680548
46-47	23.766686849465156	26.42678402606291	26.076370755623596	23.73015836884834
48-49	23.67070345554677	26.561459590494817	25.95576785689514	23.812069097063272
50-51	23.560448774168822	26.740378533441948	25.959675527900117	23.739497164489112
52-53	23.836780591717822	26.50468677727545	25.849647716824407	23.80888491418232
54-55	23.64874779594964	26.471514511781848	25.911330796665993	23.96840689560252
56-57	23.754900386178715	26.542574485761545	25.86996417383396	23.83256095422578
58-59	23.685790008620472	26.26516846333872	25.985333711070506	24.063707816970297
60-61	23.64842472367233	26.36914276920476	26.153087532932272	23.829344974190633
62-63	23.55148723760209	26.49737130629191	26.124754460889104	23.826386995216893
64-65	23.553152729776176	26.360834566008677	26.057232263781543	24.0287804404336
66-67	23.61196184382498	26.48095767577655	25.9392631029094	23.96781737748907
68-69	23.795924082558763	26.189618466348385	26.03522502686658	23.979232424226264
70-71	23.565689033045935	26.23861300573731	26.15865179635274	24.037046164864016
72-73	23.718018056489772	26.273495406323043	25.973442666233705	24.035043870953476
74-75	23.57315592127694	26.39002893841262	26.057468033035434	23.979347107275007
76-77	23.66320864604859	26.185608617264265	26.133697125598932	24.01748561108821
78-79	23.682552976401805	26.229815907464314	26.161171894405697	23.926459221728184
80-81	23.70458709590858	26.370810717782415	26.008073212501753	23.916528973807253
82-83	23.62499810344566	26.322656996768274	26.024843778109645	24.027501121676423
84-85	23.64874472108333	26.41133804227295	25.930350812244402	24.009566424399324
86-87	23.59966304683354	26.236412619355644	26.093255213843	24.070669119967814
88-89	23.55489230915952	26.167779663478314	26.094779210476695	24.182548816885475
90-91	23.511609120487282	26.253988972305482	26.124417138075067	24.109984769132165
92-93	23.552760866120583	26.210177283203777	26.159910156452366	24.07715169422327
94-95	23.401721099212804	26.129963127353584	26.39761549227725	24.07070028115637
96-97	23.63224017456553	25.916205683697296	26.281827441183083	24.16972670055409
98-99	23.53612078293197	26.087061126678503	26.104639468427475	24.272178621962045
100-101	23.337799728461096	26.13983581499716	26.285902327070627	24.23646212947112
102-103	23.22822834539847	25.964604018128483	26.444391553797157	24.362776082675886
104-105	23.22705612241359	26.19520677408838	26.154607156977672	24.423129946520355
106-107	23.158815957253548	26.252960735541443	26.206959045488777	24.381264261716233
108-109	23.24132160461981	26.160435968247498	26.23517833685224	24.363064090280456
110-111	23.11132673918967	26.319842198400522	26.239697057001905	24.3291340054079
112-113	23.152975336878846	26.4427353643459	26.108587614511062	24.29570168426419
114-115	23.13858163733801	26.457028450580754	26.135966055092407	24.268423856988825
116-117	23.04146636360917	26.395112872732756	26.17995823494297	24.383462528715103
118-119	23.06562008166935	26.284707794396468	26.05938986201525	24.59028226191893
120-121	23.06448263588885	26.47908273481879	26.114143122396964	24.3422915068954
122-123	23.065826400661912	26.464195594434443	26.053188378560165	24.41678962634348
124-125	23.02980436567997	26.50543698546747	25.96474205846193	24.50001659039063
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	741.0
1	553.0
2	315.5
3	241.5
4	199.0
5	170.0
6	169.5
7	203.5
8	227.0
9	242.0
10	252.0
11	277.5
12	319.5
13	368.0
14	430.0
15	474.5
16	545.0
17	641.0
18	772.5
19	972.0
20	1184.0
21	1513.0
22	2184.5
23	2936.5
24	3762.0
25	4933.0
26	6706.5
27	9208.5
28	11400.5
29	13881.0
30	17466.0
31	21786.5
32	25999.5
33	29601.5
34	32920.0
35	36201.5
36	40759.5
37	46244.5
38	50994.5
39	54330.0
40	58636.5
41	63699.0
42	67878.0
43	72952.0
44	77168.0
45	80576.5
46	85581.0
47	89345.0
48	92112.5
49	94891.0
50	98300.0
51	99393.0
52	95977.5
53	93829.0
54	91671.0
55	87757.5
56	82325.0
57	75406.0
58	69472.5
59	62280.5
60	52361.5
61	43342.0
62	35847.0
63	27713.0
64	20549.0
65	15602.5
66	11729.0
67	8989.5
68	6936.5
69	5266.5
70	4190.0
71	3281.5
72	2500.5
73	2001.0
74	1543.5
75	1157.5
76	877.5
77	600.0
78	394.0
79	263.5
80	167.0
81	119.5
82	82.5
83	61.5
84	49.0
85	39.5
86	36.5
87	28.5
88	20.0
89	13.0
90	8.0
91	6.0
92	5.0
93	5.5
94	5.0
95	4.0
96	4.5
97	6.0
98	6.5
99	4.5
100	5.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.033725862491248836
2	0.017079678433871516
3	0.021804767137658308
4	0.019854042443434403
5	0.01291813241952719
6	0.005201932517930411
7	0.00455169095318911
8	0.005808824645022292
9	0.002991111197809986
10-11	0.00476843814143621
12-13	0.006350692615640043
14-15	0.0021457971636462945
16-17	7.586151588648516E-4
18-19	2.1674718824710048E-5
20-21	0.0027526892907381757
22-23	9.103381906378219E-4
24-25	0.001430531442430863
26-27	8.453140341636918E-4
28-29	0.0018857005377497739
30-31	0.0011921095353590525
32-33	0.0
34-35	0.0054186797061775115
36-37	0.005873848801496422
38-39	8.88663471813112E-4
40-41	8.453140341636918E-4
42-43	0.0016472786306779635
44-45	8.66988752988402E-4
46-47	0.01801169134333405
48-49	0.002210821320120425
50-51	0.008496489779286338
52-53	0.0011270853788849225
54-55	1.0837359412355024E-4
56-57	6.502415647413014E-5
58-59	0.0050502094861574415
60-61	7.586151588648516E-4
62-63	0.007629501026297936
64-65	0.008626538092234598
66-67	0.0
68-69	0.002861062884861726
70-71	0.003966473544921938
72-73	0.010425539754685532
74-75	0.0017123027871520937
76-77	0.0010403865035860823
78-79	2.6009662589652056E-4
80-81	0.003056135354284116
82-83	8.66988752988402E-4
84-85	7.369404400401415E-4
86-87	0.012267890854785886
88-89	0.0
90-91	2.817713447212306E-4
92-93	0.006870885867433085
94-95	0.004833462297910341
96-97	0.0034462802931288974
98-99	8.236393153389817E-4
100-101	0.015237327333771162
102-103	0.008193043715740398
104-105	0.007065958336855475
106-107	0.017253076184469196
108-109	0.010620612224107923
110-111	0.017101353152696227
112-113	0.003077810073108827
114-115	0.011877745915941106
116-117	0.0052886313932292515
118-119	0.004096521857870199
120-121	0.006589114522711854
122-123	0.007997971246318007
124-125	0.05563900322303069
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2306835.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.86888248914584
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.86699556013834	37.276607475567815
2	13.429530978614768	13.931495284282262
3	5.652869319643939	8.79624043401328
4	2.8386562239377273	5.889517044260337
5	1.6976774712358245	4.402831663000063
6	1.0852787151390961	3.3775316486112574
7	0.7367951634283517	2.675171922530533
8	0.5160766028246622	2.1414653333847995
9	0.3858041935135728	1.801010913945767
>10	1.7028886253300648	14.74220783313626
>50	0.06295313797336524	2.1909574650281916
>100	0.0230413320588677	2.128541997564671
>500	0.001179850956519287	0.42252537448389405
>1k	2.528252049683203E-4	0.223895610190964
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	4.3349437649420096E-5	0.0
3	0.0	0.0	0.0	4.3349437649420096E-5	0.0
4	0.0	0.0	0.0	4.3349437649420096E-5	0.0
5	4.3349437649420096E-5	0.0	0.0	4.3349437649420096E-5	4.3349437649420096E-5
6	8.669887529884019E-5	0.0	0.0	4.3349437649420096E-5	8.669887529884019E-5
7	1.7339775059768038E-4	0.0	0.0	4.3349437649420096E-5	8.669887529884019E-5
8	1.7339775059768038E-4	0.0	0.0	4.3349437649420096E-5	8.669887529884019E-5
9	1.7339775059768038E-4	0.0	0.0	4.3349437649420096E-5	8.669887529884019E-5
10-11	2.1674718824710046E-4	0.0	0.0	4.3349437649420096E-5	8.669887529884019E-5
12-13	2.384219070718105E-4	0.0	0.0	6.502415647413014E-5	2.817713447212306E-4
14-15	3.4679550119536077E-4	0.0	0.0	8.669887529884019E-5	3.251207823706507E-4
16-17	4.76843814143621E-4	0.0	0.0	1.3004831294826028E-4	4.1181965766949087E-4
18-19	5.635426894424612E-4	0.0	0.0	2.6009662589652056E-4	4.334943764942009E-4
20-21	6.502415647413014E-4	0.0	0.0	3.901449388447808E-4	4.76843814143621E-4
22-23	7.369404400401415E-4	0.0	0.0	7.586151588648516E-4	4.76843814143621E-4
24-25	8.236393153389817E-4	0.0	0.0	0.0013221578483073129	4.76843814143621E-4
26-27	8.236393153389817E-4	0.0	0.0	0.0021241224448215845	4.76843814143621E-4
28-29	8.886634718131118E-4	0.0	0.0	0.00459504039083853	4.98518532968331E-4
30-31	9.103381906378219E-4	0.0	0.0	0.013416650952495519	5.201932517930411E-4
32-33	9.103381906378219E-4	0.0	0.0	0.031059872075809496	6.068921270918813E-4
34-35	0.0011487600977096325	0.0	0.0	0.05375330268528092	7.802898776895616E-4
36-37	0.0013438325671320229	0.0	0.0	0.09183578366029646	7.802898776895616E-4
38-39	0.001430531442430863	0.0	0.0	0.1512678626776514	9.103381906378219E-4
40-41	0.001473880880080283	0.0	0.0	0.22142892751323784	9.103381906378219E-4
42-43	0.0015605797553791233	0.0	0.0	0.2887939536204367	9.103381906378219E-4
44-45	0.0016906280683273837	0.0	0.0	0.3627047448126979	9.32012909462532E-4
46-47	0.001907375256574484	0.0	0.0	0.4532617200623365	9.75362347111952E-4
48-49	0.001994074131873324	0.0	0.0	0.5491073267052042	0.0010620612224107923
50-51	0.001994074131873324	0.0	0.0	0.6482691653282528	0.0011270853788849225
52-53	0.001994074131873324	0.0	0.0	0.7484063662984132	0.0012137842541837627
54-55	0.001994074131873324	0.0	0.0	0.856433164920768	0.0012137842541837627
56-57	0.0020374235695227445	0.0	0.0	0.9836854391406408	0.0012137842541837627
58-59	0.0020374235695227445	0.0	0.0	1.120084444704541	0.0012137842541837627
60-61	0.0020374235695227445	0.0	0.0	1.2691848354997215	0.0012137842541837627
62-63	0.0020807730071721645	0.0	0.0	1.4198241313314564	0.0012571336918331827
64-65	0.0021457971636462945	0.0	0.0	1.5777461326882936	0.0012788084106578929
66-67	0.002384219070718105	0.0	0.0	1.7603122893488266	0.0013004831294826029
68-69	0.0024709179460169453	0.0	0.0	1.9669373839047872	0.0013004831294826029
70-71	0.0025359421024910758	0.0	0.0	2.194955425940737	0.0013004831294826029
72-73	0.0026876651342640457	0.0	0.0	2.42234490113077	0.0014738808800802833
74-75	0.0027526892907381757	0.0	0.0	2.6540693200857453	0.0016906280683273837
76-77	0.002774364009562886	0.0	0.0	2.904672419137043	0.0017339775059768037
78-79	0.002817713447212306	0.0	0.0	3.18969497168198	0.0017556522248015137
80-81	0.003251207823706507	0.0	0.0	3.4963055441763284	0.001799001662450934
82-83	0.003294557261355927	0.0	0.0	3.8178499979409017	0.001864025818925064
84-85	0.0034246055743041874	0.0	0.0	4.1488012796754	0.001864025818925064
86-87	0.003576328606077158	0.0	0.0	4.491305186543467	0.001929049975399194
88-89	0.0036413527625512878	0.0	0.0	4.877830447344522	0.001994074131873324
90-91	0.003923124107272518	0.0	0.0	5.297799799292104	0.001994074131873324
92-93	0.003944798826097228	0.0	0.0	5.729646897155627	0.0021241224448215845
94-95	0.003944798826097228	0.0	0.0	6.1611038500803055	0.0021241224448215845
96-97	0.003944798826097228	0.0	0.0	6.614669016206188	0.002254170757769845
98-99	0.004053172420220779	0.0	0.0	7.098990608344334	0.002340869633068685
100-101	0.004204895451993749	0.0	0.0	7.6442831845363886	0.002340869633068685
102-103	0.004356618483766719	0.0	0.0	8.199762878576058	0.002362544351893395
104-105	0.004833462297910341	0.0	0.0	8.754830752958057	0.002384219070718105
106-107	0.0051585830802809915	0.0	0.0	9.318503490713466	0.002384219070718105
108-109	0.0052236072367551215	0.0	0.0	9.910418387097472	0.002384219070718105
110-111	0.0053753302685280915	0.0	0.0	10.543753671155502	0.0024275685083675253
112-113	0.0054186797061775115	0.0	0.0	11.20981778063884	0.0024275685083675253
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACATG	10300	0.0	27.160915	1
CATGGGG	6350	0.0	26.801744	4
TACATGG	10615	0.0	25.565441	2
GCGTTAG	165	1.1071603E-5	25.25212	1
ACATGGG	10765	0.0	24.87697	3
GTATCAA	6465	0.0	24.214249	1
GTATCGC	185	2.6925829E-5	22.512886	6
TCAACGC	6955	0.0	21.39014	4
CAACGCA	7015	0.0	21.120987	5
ATCAACG	7265	0.0	20.560661	3
AACGCAG	7180	0.0	20.550959	6
TATCAAC	7390	0.0	20.535446	2
GGTATCA	5135	0.0	19.473885	1
AACGCGG	190	8.32672E-4	18.78895	6
GTATATA	1210	0.0	18.69313	1
GTATAAG	965	0.0	18.504515	1
ATGGGGG	3285	0.0	18.294836	5
CGCAGAG	8260	0.0	17.57578	8
ACGCAGA	8235	0.0	17.556887	7
ATGGGGA	3695	0.0	17.55313	5
>>END_MODULE