FastQCFastQC Report
Mon 15 Aug 2016
SRR2921011_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921011_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2306835
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30730.13321282189666794No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA76250.036.570391
TCAACGC96250.028.5293464
CAACGCA97150.028.1713285
TATCAAC97250.028.1710642
ATCAACG98700.027.6998673
AACGCAG100750.027.3383126
GGTATCA53450.026.0850561
CATGGGG64200.025.0507284
GTACATG104200.024.9883371
TACATGG105950.024.2838672
ACGCAGA114150.023.8705837
AGTACTT58600.023.5580412-13
CGCAGAG115600.023.5212338
ACATGGG106000.023.375973
AGAGTAC113850.020.7478610-11
GAGTACT55700.020.72503712-13
GCAGAGT129750.020.4975459
CGCGATA750.004714878719.828337112-113
TACTTTT72050.018.91255814-15
GTACTTT62550.018.31269314-15
Produced by FastQC (version 0.11.5)