##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921011_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2306835
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.951700056571017	31.0	30.0	34.0	26.0	34.0
2	30.085138729037837	31.0	30.0	34.0	26.0	34.0
3	30.208235526164636	31.0	30.0	34.0	26.0	34.0
4	33.76042109643733	35.0	35.0	37.0	29.0	37.0
5	33.307165011801885	35.0	33.0	37.0	27.0	37.0
6	33.13231938998671	35.0	33.0	37.0	26.0	37.0
7	32.960086438778674	35.0	33.0	37.0	26.0	37.0
8	32.893684637176044	35.0	33.0	37.0	26.0	37.0
9	34.21254576075012	38.0	34.0	39.0	26.0	39.0
10-11	34.10047185862881	38.0	33.5	39.0	25.0	39.0
12-13	33.9940873534518	38.0	33.5	39.0	25.0	39.0
14-15	34.90227822969567	39.0	34.0	41.0	25.0	41.0
16-17	34.88592812229743	39.0	34.0	41.0	25.0	41.0
18-19	34.92386559940351	39.0	34.0	41.0	25.0	41.0
20-21	34.86757028569447	39.0	34.0	41.0	24.0	41.0
22-23	34.83253331946152	39.0	34.0	41.0	24.0	41.0
24-25	34.799042627669515	39.0	34.0	41.0	24.0	41.0
26-27	34.65548077777561	39.0	34.0	41.0	23.5	41.0
28-29	34.527234284203246	39.0	33.5	41.0	23.0	41.0
30-31	34.35658900614912	38.0	33.0	41.0	22.5	41.0
32-33	34.20776453452457	38.0	33.0	40.5	21.5	41.0
34-35	34.078299921754265	38.0	33.0	40.0	20.0	41.0
36-37	33.91093316166956	38.0	33.0	40.0	18.5	41.0
38-39	33.78293310965023	38.0	33.0	40.0	18.0	41.0
40-41	33.59486460886886	38.0	33.0	40.0	16.0	41.0
42-43	33.41720018987054	38.0	32.0	40.0	15.0	41.0
44-45	33.19680319572054	38.0	32.0	40.0	13.5	41.0
46-47	33.0173742378627	38.0	32.0	40.0	9.0	41.0
48-49	32.78946847087026	38.0	32.0	40.0	8.5	41.0
50-51	32.5839037035592	37.5	31.0	40.0	7.5	41.0
52-53	32.35288783116261	37.0	31.0	40.0	6.0	41.0
54-55	32.14766097271803	37.0	31.0	40.0	2.0	41.0
56-57	32.01585505682028	37.0	31.0	40.0	2.0	41.0
58-59	31.939226906128958	36.5	31.0	40.0	2.0	41.0
60-61	31.656309619023467	36.0	30.5	40.0	2.0	41.0
62-63	31.399597500471426	36.0	30.0	39.0	2.0	41.0
64-65	31.12619649866592	35.0	30.0	39.0	2.0	41.0
66-67	30.803028608461375	35.0	30.0	39.0	2.0	40.5
68-69	30.48882321449085	35.0	29.0	38.0	2.0	40.0
70-71	30.14758185999432	35.0	29.0	37.0	2.0	40.0
72-73	29.86241777153546	35.0	29.0	37.0	2.0	39.0
74-75	29.60328155243006	34.5	29.0	36.5	2.0	39.0
76-77	29.28097501555161	34.0	29.0	36.0	2.0	39.0
78-79	28.974203183149207	34.0	29.0	35.5	2.0	37.5
80-81	28.72645486131431	34.0	29.0	35.0	2.0	37.0
82-83	28.47949116430087	34.0	28.0	35.0	2.0	36.5
84-85	28.288151081460096	34.0	28.0	35.0	2.0	36.0
86-87	28.057577373327526	34.0	27.0	35.0	2.0	36.0
88-89	27.93036736480936	34.0	27.0	35.0	2.0	35.5
90-91	27.760916797256847	34.0	27.0	35.0	2.0	35.0
92-93	27.90217765900032	34.0	27.5	35.0	2.0	35.0
94-95	27.84683278171174	34.0	27.5	35.0	2.0	35.0
96-97	27.78860234910603	34.0	27.0	35.0	2.0	35.0
98-99	27.722342733658888	34.0	27.0	35.0	2.0	35.0
100-101	27.603771401075498	34.0	27.0	35.0	2.0	35.0
102-103	26.308050424065875	31.5	24.5	34.0	2.0	35.0
104-105	27.36197365654674	33.5	26.5	35.0	2.0	35.0
106-107	27.568296822269474	34.0	27.0	35.0	2.0	35.0
108-109	27.547885522805053	34.0	27.0	35.0	2.0	35.0
110-111	27.489745257029654	34.0	27.0	35.0	2.0	35.0
112-113	27.389852763635027	34.0	27.0	35.0	2.0	35.0
114-115	27.304946387582987	34.0	27.0	35.0	2.0	35.0
116-117	27.175107018924194	34.0	27.0	35.0	2.0	35.0
118-119	27.03752348997653	34.0	26.5	35.0	2.0	35.0
120-121	26.910001582254473	34.0	26.0	35.0	2.0	35.0
122-123	26.679380406487677	34.0	25.0	35.0	2.0	35.0
124-125	25.581224491565283	32.5	22.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	73905.0
3	24759.0
4	12629.0
5	10020.0
6	10924.0
7	13103.0
8	14559.0
9	15743.0
10	16562.0
11	17371.0
12	17880.0
13	19074.0
14	20457.0
15	17286.0
16	13086.0
17	11798.0
18	10785.0
19	10563.0
20	10260.0
21	10765.0
22	12593.0
23	16136.0
24	21705.0
25	28795.0
26	35162.0
27	41284.0
28	48494.0
29	57081.0
30	67272.0
31	80359.0
32	97310.0
33	120376.0
34	156029.0
35	215107.0
36	347218.0
37	464551.0
38	145486.0
39	348.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.90493043574056	19.46983871709262	13.395771582529434	25.229459264637384
2	16.300833220263453	20.725730904664534	37.449351556874724	25.52408431819729
3	19.462550459861	24.1681058998155	29.246127942624884	27.123215697698615
4	13.558402461170932	16.185549648776185	35.18049917879949	35.0755487112534
5	13.894728536243075	37.46182070112475	34.2037527026283	14.439698060003876
6	33.93510803766969	35.88617592947423	16.197413893257636	13.981302139598437
7	28.930161340875898	31.724462020062155	20.772643190731817	18.572733448330126
8	26.891907534248062	35.32985610590703	19.181341413241064	18.59689494660385
9	26.51143975053158	15.279507306802454	18.936030358003837	39.27302258466213
10-11	25.714465956086595	25.754896483758095	27.244712651676807	21.28592490847851
12-13	26.241885664833514	22.809216219825426	27.701728919204378	23.24716919613668
14-15	23.250110950409773	24.271960430324985	24.917846831414565	27.560081787850677
16-17	23.3687614414634	27.43793829355757	25.7390239465506	23.454276318428434
18-19	23.462997787718553	26.38818329910726	27.50134920330331	22.647469709870876
20-21	24.036070972995333	25.49264159435157	27.433572297603735	23.037715135049357
22-23	23.72319299310584	25.284199800546332	27.36729826995621	23.625308936391622
24-25	23.37560388044403	25.702933746803367	27.371447500665557	23.550014872087043
26-27	23.2767292325169	25.799459757107492	27.39352315543473	23.53028785494088
28-29	23.388609939296394	25.83502954100393	27.45601801618604	23.32034250351364
30-31	23.535566304930356	25.71738779786011	27.31794956735597	23.429096329853564
32-33	23.327879361939548	25.797038985669722	27.260700772090697	23.614380880300033
34-35	23.38849187429984	25.8661490986898	27.348736791143587	23.396622235866776
36-37	23.681884920824203	25.611044284503848	27.2549426876322	23.452128107039748
38-39	23.62583861586618	25.744387780876554	27.176997246224317	23.45277635703295
40-41	23.573325435309197	25.850237330999775	27.124314090427752	23.45212314326328
42-43	23.703999373840993	25.667857770843582	27.079534118619407	23.548608736696018
44-45	23.58508708465596	25.796822488141082	26.966898226089036	23.651192201113922
46-47	23.629978672897405	25.846492563267947	26.903849372892985	23.619679390941663
48-49	23.60464652403959	26.127353160283313	26.520908837831296	23.7470914778458
50-51	23.54524046864492	26.343000872823197	26.43730740330112	23.67445125523076
52-53	23.786766236289086	26.1477795430736	26.381671604098877	23.683782616538434
54-55	23.584877842754473	26.1490482306691	26.36594878163639	23.90012514494004
56-57	23.615624798103592	26.19157361907194	26.306174897246926	23.88662668557754
58-59	23.48765497006671	25.983263026829935	26.51094663260503	24.018135370498324
60-61	23.513605989419467	26.149632274658458	26.624342511252497	23.71241922466958
62-63	23.406343076037327	26.219992041609157	26.609519493178446	23.76414538917507
64-65	23.343064208816507	26.06869233382938	26.56012937301229	24.02811408434182
66-67	23.352171237590465	26.27421466451054	26.434124360523835	23.939489737375155
68-69	23.579845415027048	25.902609298947393	26.525567227118497	23.991978058907065
70-71	23.394115874217	25.94249436803865	26.58983926956876	24.07355048817559
72-73	23.518711818865032	25.95276391628783	26.391368586208973	24.137155678638166
74-75	23.38206444057147	26.10346332462601	26.374460688146854	24.140011546655664
76-77	23.42178909320471	25.88550967223595	26.556737764699022	24.135963469860314
78-79	23.450554887425724	26.017777818248593	26.4709714752285	24.06069581909718
80-81	23.450124193856453	26.04743106140707	26.30199757314126	24.200447171595215
82-83	23.31343534063035	26.032545804736877	26.363702002353413	24.29031685227936
84-85	23.34585237131854	26.098183539907577	26.208270716464167	24.347693372309713
86-87	23.307624144421276	25.91055214300938	26.34476735652111	24.437056356048238
88-89	23.24394231102786	25.907547611614113	26.329244284871823	24.519265792486213
90-91	23.183809328056935	25.956889456653997	26.36998627130095	24.489314943988123
92-93	23.147292179585353	25.926570712751374	26.368609419810372	24.5575276878529
94-95	23.033298032512164	25.774643459899604	26.60688290135895	24.585175606229274
96-97	23.229537661637195	25.619586214850298	26.50183388804861	24.6490422354639
98-99	23.17056672887341	25.67632489957889	26.313170774855525	24.839937596692174
100-101	22.89710012051847	25.641968437992606	26.58317888223292	24.877752559256
102-103	22.784980549589278	25.512395775045153	26.63345834871726	25.069165326648307
104-105	22.81892304213573	25.58900638484547	26.407387871720346	25.184682701298456
106-107	22.665137076709936	25.654969509002925	26.470685785674014	25.20920762861313
108-109	22.6991647988977	25.60900396518373	26.49644984793734	25.19538138798123
110-111	22.60243829681698	25.712550143862806	26.47628474950181	25.208726809818398
112-113	22.534774052658253	25.780446236841183	26.345146590142	25.339633120358563
114-115	22.49473068334523	25.8634948375546	26.3533684064715	25.288406072628668
116-117	22.34113512274969	25.73794944686714	26.445826840031415	25.475088590351753
118-119	22.391396566594867	25.659454436402758	26.31273652971471	25.636412467287666
120-121	22.287375154955576	25.822085529192123	26.34354260907149	25.546996706780806
122-123	22.20829924841952	25.839507633936915	26.29044725042582	25.66174586721775
124-125	22.06001032798622	25.88314367243844	26.233063306304604	25.823782693270736
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	27.0
1	37.5
2	124.0
3	235.5
4	200.5
5	152.0
6	238.0
7	443.0
8	679.0
9	718.0
10	584.0
11	535.5
12	573.5
13	636.0
14	738.5
15	852.0
16	977.5
17	1094.5
18	1258.0
19	1487.5
20	1731.0
21	2089.5
22	2715.5
23	3440.0
24	4181.5
25	5327.5
26	7152.5
27	9622.0
28	11890.5
29	14404.0
30	17903.0
31	22345.0
32	26695.0
33	29757.0
34	32528.5
35	36289.5
36	40977.5
37	46176.5
38	50793.0
39	54179.5
40	58202.5
41	63312.5
42	67753.5
43	72695.0
44	77311.5
45	80659.5
46	85467.5
47	89840.0
48	92643.5
49	95393.0
50	99681.5
51	100510.0
52	96226.5
53	93781.5
54	91943.5
55	87993.0
56	82048.5
57	74834.5
58	68706.0
59	61689.5
60	51426.5
61	42121.5
62	34798.5
63	26579.0
64	19546.5
65	14835.5
66	10939.0
67	8138.0
68	6314.0
69	4798.5
70	3654.0
71	2817.0
72	2182.5
73	1799.0
74	1390.0
75	1005.0
76	712.0
77	455.5
78	308.5
79	207.5
80	114.0
81	67.0
82	51.0
83	32.5
84	20.5
85	13.0
86	9.0
87	7.0
88	4.0
89	5.5
90	3.5
91	1.0
92	0.5
93	0.5
94	1.5
95	2.5
96	2.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05188927686635585
2	8.669887529884019E-5
3	0.002210821320120425
4	0.12532322424447348
5	0.012311240292435308
6	0.002037423569522744
7	0.00450834151553969
8	0.00901668303107938
9	0.021284573885865264
10-11	0.01775159471743753
12-13	0.018336812125704698
14-15	0.02707172381206285
16-17	0.034831273151309045
18-19	0.036608600094935266
20-21	0.021371272761164108
22-23	0.023191949142439747
24-25	0.021718068262359468
26-27	0.020157488506980344
28-29	0.020135813788155633
30-31	0.02388554014483047
32-33	0.024470757553097643
34-35	0.028805701318039653
36-37	0.030387955792243487
38-39	0.03158006532760254
40-41	0.03350911530300173
42-43	0.030843124887562395
44-45	0.028588954129792555
46-47	0.03680367256435766
48-49	0.029520967039255084
50-51	0.02280180420359497
52-53	0.02787368840857712
54-55	0.030734751293438847
56-57	0.025055974961364816
58-59	0.027028374374413428
60-61	0.03910119275977692
62-63	0.04735926063199145
64-65	0.04094354385987728
66-67	0.036673624251409395
68-69	0.028610628848617262
70-71	0.03467955011953608
72-73	0.0384076017573862
74-75	0.02338702161186214
76-77	0.020179163225805055
78-79	0.02802541144035009
80-81	0.02434070924014938
82-83	0.03569826190429745
84-85	0.020851079509371064
86-87	0.031233269826407173
88-89	0.02926087041335856
90-91	0.031081546794634207
92-93	0.025532818775508434
94-95	0.020265862101103895
96-97	0.017188052027995067
98-99	0.02371214239423279
100-101	0.02382051598835634
102-103	0.025381095743735465
104-105	0.022303285670626637
106-107	0.01450038689373102
108-109	0.016667858776202027
110-111	0.020417585132876864
112-113	0.013091530170124868
114-115	0.01393684420428856
116-117	0.012636361074805957
118-119	0.007564476869823806
120-121	0.0054403544250022215
122-123	0.004161546014344329
124-125	0.06279166043518501
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2306835.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.62171136509607
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.84832431284383	34.65993388387559
2	14.238654309441486	14.130927887409733
3	6.037745175384683	8.988097451668201
4	3.1202459071515904	6.193277671711942
5	1.8426396666373326	4.571746684387728
6	1.170313547460542	3.48437766352492
7	0.7973397866803937	2.7695755328193243
8	0.5673384360507366	2.252184329602773
9	0.42137580077485237	1.8818449526057353
>10	1.857131146438316	15.404907694321935
>50	0.07171577907269343	2.4126386228242582
>100	0.025413495806414433	2.3176731060209153
>500	0.001321977192774315	0.44401621255102264
>1k	4.406590642579036E-4	0.4887983066759629
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3073	0.13321282189666794	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.3004831294826028E-4
2	0.0	0.0	0.0	8.669887529884019E-5	1.3004831294826028E-4
3	0.0	0.0	0.0	8.669887529884019E-5	1.3004831294826028E-4
4	0.0	0.0	0.0	8.669887529884019E-5	1.3004831294826028E-4
5	0.0	0.0	0.0	1.3004831294826028E-4	1.3004831294826028E-4
6	4.3349437649420096E-5	0.0	0.0	1.3004831294826028E-4	1.3004831294826028E-4
7	8.669887529884019E-5	0.0	0.0	1.3004831294826028E-4	1.3004831294826028E-4
8	8.669887529884019E-5	0.0	0.0	1.3004831294826028E-4	1.3004831294826028E-4
9	8.669887529884019E-5	0.0	0.0	2.1674718824710046E-4	1.3004831294826028E-4
10-11	8.669887529884019E-5	0.0	0.0	3.0344606354594067E-4	1.3004831294826028E-4
12-13	1.3004831294826028E-4	0.0	0.0	3.0344606354594067E-4	2.6009662589652056E-4
14-15	1.3004831294826028E-4	0.0	0.0	3.901449388447808E-4	3.0344606354594067E-4
16-17	1.7339775059768038E-4	0.0	0.0	4.334943764942009E-4	4.1181965766949087E-4
18-19	1.7339775059768038E-4	0.0	0.0	5.418679706177512E-4	4.76843814143621E-4
20-21	1.7339775059768038E-4	0.0	0.0	6.935910023907214E-4	6.502415647413014E-4
22-23	1.7339775059768038E-4	0.0	0.0	0.0010620612224107923	7.369404400401415E-4
24-25	3.0344606354594067E-4	0.0	0.0	0.0016472786306779635	7.369404400401415E-4
26-27	3.0344606354594067E-4	0.0	0.0	0.0024709179460169453	7.369404400401415E-4
28-29	6.068921270918812E-4	0.0	0.0	0.004876811735559761	7.369404400401415E-4
30-31	7.369404400401415E-4	0.0	0.0	0.013460000390144937	7.369404400401415E-4
32-33	7.802898776895616E-4	0.0	0.0	0.030821450168737687	8.453140341636918E-4
34-35	8.886634718131118E-4	0.0	0.0	0.053211434714663164	8.669887529884018E-4
36-37	9.75362347111952E-4	0.0	0.0	0.09053530053081386	8.669887529884018E-4
38-39	0.0012137842541837627	0.0	0.0	0.148233402042192	8.886634718131118E-4
40-41	0.0012137842541837627	0.0	0.0	0.21676886296592518	9.103381906378219E-4
42-43	0.0013655072859567329	0.0	0.0	0.28233488741067303	9.103381906378219E-4
44-45	0.001473880880080283	0.0	0.0	0.35548706344406944	9.32012909462532E-4
46-47	0.0017773269436262237	0.0	0.0	0.44509035106542083	9.53687628287242E-4
48-49	0.0018206763812756439	0.0	0.0	0.5388985341387659	9.53687628287242E-4
50-51	0.0018206763812756439	0.0	0.0	0.6362180216617139	9.53687628287242E-4
52-53	0.0018206763812756439	0.0	0.0	0.7355749327541848	9.97037065936662E-4
54-55	0.001864025818925064	0.0	0.0	0.8420628263399853	0.0010403865035860823
56-57	0.001907375256574484	0.0	0.0	0.9679929427115506	0.0011270853788849225
58-59	0.001907375256574484	0.0	0.0	1.1027880190824226	0.0011270853788849225
60-61	0.001907375256574484	0.0	0.0	1.2510864452810886	0.0011270853788849225
62-63	0.001907375256574484	0.0	0.0	1.3996449681056513	0.0011704348165343425
64-65	0.001950724694223904	0.0	0.0	1.5563098357706555	0.0012137842541837624
66-67	0.001994074131873324	0.0	0.0	1.736643496392243	0.0013004831294826029
68-69	0.0020374235695227445	0.0	0.0	1.9397139370609513	0.0013004831294826029
70-71	0.0020590982883474545	0.0	0.0	2.1650876634002865	0.0013004831294826029
72-73	0.0021241224448215845	0.0	0.0	2.3900062206443025	0.0013221578483073129
74-75	0.0021674718824710045	0.0	0.0	2.620300108156847	0.0013655072859567329
76-77	0.0021674718824710045	0.0	0.0	2.868107168479757	0.001387182004781443
78-79	0.002189146601295715	0.0	0.0	3.1497267901692148	0.001430531442430863
80-81	0.0025359421024910753	0.0	0.0	3.455860518849419	0.0014738808800802833
82-83	0.0026443156966146257	0.0	0.0	3.776559658579829	0.0016472786306779635
84-85	0.0028393881660370157	0.0	0.0	4.10540849258833	0.0016472786306779635
86-87	0.0029694364789852766	0.0	0.0	4.444986312415063	0.0017339775059768037
88-89	0.0029911111978099866	0.0	0.0	4.825724423289919	0.0018206763812756439
90-91	0.003186183667232377	0.0	0.0	5.2435913275115045	0.0018206763812756439
92-93	0.003294557261355927	0.0	0.0	5.674137942245544	0.001950724694223904
94-95	0.003294557261355927	0.0	0.0	6.102972254192433	0.001950724694223904
96-97	0.003294557261355927	0.0	0.0	6.553719706871103	0.0021024477259968745
98-99	0.0035546538872524474	0.0	0.0	7.035505356906757	0.002210821320120425
100-101	0.003814750513148968	0.0	0.0	7.579345726937557	0.002210821320120425
102-103	0.004118196576694909	0.0	0.0	8.13525891535372	0.002232496038945135
104-105	0.00461671510966324	0.0	0.0	8.691583923427553	0.002254170757769845
106-107	0.004876811735559761	0.0	0.0	9.256752216781868	0.002254170757769845
108-109	0.004898486454384471	0.0	0.0	9.848688787884699	0.002254170757769845
110-111	0.005028534767332731	0.0	0.0	10.484083170231074	0.002297520195419265
112-113	0.005071884204982151	0.0	0.0	11.15389700607109	0.002297520195419265
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	7625	0.0	36.57039	1
TCAACGC	9625	0.0	28.529346	4
CAACGCA	9715	0.0	28.171328	5
TATCAAC	9725	0.0	28.171064	2
ATCAACG	9870	0.0	27.699867	3
AACGCAG	10075	0.0	27.338312	6
GGTATCA	5345	0.0	26.085056	1
CATGGGG	6420	0.0	25.050728	4
GTACATG	10420	0.0	24.988337	1
TACATGG	10595	0.0	24.283867	2
ACGCAGA	11415	0.0	23.870583	7
AGTACTT	5860	0.0	23.55804	12-13
CGCAGAG	11560	0.0	23.521233	8
ACATGGG	10600	0.0	23.37597	3
AGAGTAC	11385	0.0	20.74786	10-11
GAGTACT	5570	0.0	20.725037	12-13
GCAGAGT	12975	0.0	20.497545	9
CGCGATA	75	0.0047148787	19.828337	112-113
TACTTTT	7205	0.0	18.912558	14-15
GTACTTT	6255	0.0	18.312693	14-15
>>END_MODULE