FastQCFastQC Report
Mon 15 Aug 2016
SRR2921010_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921010_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2998765
Sequences flagged as poor quality0
Sequence length125
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA84850.032.267211
GTACATG142550.030.3127121
TACATGG147800.028.0232032
TCAACGC92250.027.9312274
ACATGGG149150.027.3302143
CATGGGG90200.027.1806074
CAACGCA95050.027.1070655
AACGCAG96600.026.7926356
ATCAACG99250.026.2015273
TATCAAC99850.025.9253222
GGTATCA63450.024.9520021
ACGCAGA108450.023.5907757
CGCAGAG110800.022.7678558
GCAGAGT120650.019.8734729
ATGGGGG48200.019.6289375
GTATTAC8000.019.3436641
ATGGGGA46350.018.8718435
GTATAAT21250.016.8053551
CAGAGTA114950.016.58880410-11
AGAGTAC108200.016.52392810-11
Produced by FastQC (version 0.11.5)