FastQCFastQC Report
Mon 15 Aug 2016
SRR2921010_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921010_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2998765
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39390.1313540740938353No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA100750.037.213581
GGTATCA69650.030.5522331
TCAACGC122550.030.0938194
ATCAACG126550.029.330933
CAACGCA126250.028.935785
TATCAAC129100.028.7934042
AACGCAG129650.028.1717856
CATGGGG86300.025.6821944
GTACATG143150.025.1502021
ACGCAGA148200.024.4071667
TACATGG146700.024.3644052
ACATGGG143750.024.1220993
CGCAGAG152050.023.8286888
GCAGAGT168000.020.6456479
TACCGTA3201.2207238E-820.4505397
AGTACTT81300.020.12554412-13
AGAGTAC152850.019.90999610-11
GAGTACT73650.019.02495412-13
TACACCG5101.8189894E-1218.6658615
ATGGGGG46750.018.1992155
Produced by FastQC (version 0.11.5)