FastQCFastQC Report
Mon 15 Aug 2016
SRR2921009_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921009_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3452809
Sequences flagged as poor quality0
Sequence length125
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA68400.038.9803541
TCAACGC79450.032.5036134
GGTATCA46650.032.1493951
GTACATG133100.031.3893971
TATCAAC83300.030.8602712
CAACGCA83050.030.7346555
AACGCAG84350.030.2605346
ATCAACG85850.030.1511343
TACATGG137600.029.5367872
ACATGGG138150.029.0741883
CATGGGG100050.026.7627814
ACGCAGA97000.026.0074947
CGCAGAG100650.024.8870098
GCAGAGT111100.022.0106149
AGAGTAC96700.019.5658510-11
ATGGGGA52300.018.6574735
GTATAGA11250.018.515671
ATGGGGG52800.017.3539165
CAGAGTA107850.017.2120510-11
GTATAGG7000.017.0041891
Produced by FastQC (version 0.11.5)