FastQCFastQC Report
Mon 15 Aug 2016
SRR2921009_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921009_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3452809
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46760.13542596766864312No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37160.10762251836113727No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56000.051.2856561
GTATCAA91900.045.7099271
TCAACGC119650.034.2983634
TATCAAC122800.033.6096462
CAACGCA122050.033.3894925
ATCAACG124800.033.1211553
AACGCAG128400.031.782256
ACGCAGA148450.027.0107547
CGCAGAG153850.026.1025038
GTACATG132550.025.9377941
TACATGG137350.024.629112
ACATGGG134650.024.2843533
CATGGGG96350.023.8617574
GCAGAGT166000.023.6543889
AGAGTAC152000.022.8399510-11
GAGTACT86650.020.80686212-13
AGTACTT94250.020.45484412-13
CAGAGTA163500.020.08719310-11
GTACTTT93600.019.16655214-15
TACCGTA2501.2643468E-519.0373737
Produced by FastQC (version 0.11.5)