FastQCFastQC Report
Mon 15 Aug 2016
SRR2921008_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921008_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2270213
Sequences flagged as poor quality0
Sequence length125
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA58900.035.6668971
TCAACGC69100.030.3092254
ATCAACG70000.029.9208533
TATCAAC69600.029.836342
GGTATCA38000.029.7560921
CAACGCA70450.029.558865
AACGCAG71050.029.309896
CATGGGG61450.027.4015274
TACATGG101700.026.6792282
ACATGGG99100.026.5385973
GTACATG108050.026.49271
ACGCAGA80850.025.6835847
CGCAGAG82850.024.6321478
GCGTAAG2001.8239043E-623.8048741
GCAGAGT95550.020.2373359
AGAGTAC77100.020.0250710-11
GTATAAT15850.019.8999751
ATGGGGA36650.019.805535
GTGTAGC17750.019.7815151
GTGTAGG9950.019.1395971
Produced by FastQC (version 0.11.5)