##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921008_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2270213
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.193957130894766	31.0	28.0	33.0	16.0	34.0
2	28.24950302020119	31.0	28.0	34.0	16.0	34.0
3	28.37316674690877	31.0	28.0	34.0	16.0	34.0
4	31.784053302487475	35.0	32.0	37.0	18.0	37.0
5	31.87736216821946	35.0	32.0	37.0	19.0	37.0
6	31.82200348601651	35.0	32.0	37.0	18.0	37.0
7	31.71148522187125	35.0	32.0	37.0	17.0	37.0
8	31.68622459654667	35.0	32.0	37.0	17.0	37.0
9	32.80338893310892	37.0	32.0	39.0	17.0	39.0
10-11	32.758011472932274	37.0	32.0	39.0	16.5	39.0
12-13	32.656519894829245	37.0	32.0	39.0	15.0	39.0
14-15	33.53977203901132	38.0	32.0	40.0	14.0	41.0
16-17	33.42830430448596	38.0	32.0	40.0	12.0	41.0
18-19	33.49250400733323	38.0	32.0	40.0	13.0	41.0
20-21	33.526906506129606	38.0	32.0	40.0	13.0	41.0
22-23	33.46381242641109	38.0	32.0	40.0	13.0	41.0
24-25	33.38858930858029	38.0	32.0	40.0	12.0	41.0
26-27	33.25039302479547	38.0	32.0	40.0	10.5	41.0
28-29	33.18412501382029	38.0	32.0	40.0	10.0	41.0
30-31	33.04137232938055	38.0	31.5	40.0	9.5	41.0
32-33	32.961408026471524	38.0	31.0	40.0	9.0	41.0
34-35	32.7876994361322	38.0	31.0	40.0	8.0	41.0
36-37	32.62566772368936	38.0	31.0	40.0	8.0	41.0
38-39	32.477213812096046	38.0	30.5	40.0	6.5	41.0
40-41	32.38981892888465	38.0	30.5	40.0	2.0	41.0
42-43	32.16269288388358	38.0	30.0	40.0	2.0	41.0
44-45	31.98178783224305	37.5	30.0	40.0	2.0	41.0
46-47	31.772941129312535	37.0	30.0	40.0	2.0	41.0
48-49	31.595112000503917	37.0	30.0	40.0	2.0	41.0
50-51	31.359611851398967	37.0	30.0	40.0	2.0	41.0
52-53	31.164646445069252	36.5	29.0	40.0	2.0	41.0
54-55	30.942941257053853	36.0	29.0	40.0	2.0	41.0
56-57	30.738910622042955	36.0	29.0	39.5	2.0	41.0
58-59	30.478938980615474	35.5	28.0	39.0	2.0	41.0
60-61	30.297756862461803	35.0	28.0	39.0	2.0	41.0
62-63	30.191217960605456	35.0	28.0	39.0	2.0	41.0
64-65	29.968535551509923	35.0	28.0	39.0	2.0	40.5
66-67	29.704501295693397	35.0	28.0	38.5	2.0	40.0
68-69	29.39221716200198	34.5	28.0	38.0	2.0	40.0
70-71	29.06783746723325	34.0	28.0	37.0	2.0	40.0
72-73	28.786203321009967	34.0	27.0	37.0	2.0	39.0
74-75	28.48353017976727	34.0	27.0	36.0	2.0	39.0
76-77	28.207268216682753	34.0	26.5	36.0	2.0	39.0
78-79	27.886888366862493	34.0	26.0	35.0	2.0	37.5
80-81	27.573898352269147	34.0	26.0	35.0	2.0	37.0
82-83	27.295397171983424	33.5	26.0	35.0	2.0	36.5
84-85	27.04422822880496	33.0	25.5	35.0	2.0	36.0
86-87	26.969107744515604	33.0	25.5	35.0	2.0	36.0
88-89	27.052226376996344	34.0	26.0	35.0	2.0	36.0
90-91	26.965413377511272	34.0	26.0	35.0	2.0	35.0
92-93	26.816283317908933	33.0	25.0	35.0	2.0	35.0
94-95	26.721815970571924	33.0	25.0	35.0	2.0	35.0
96-97	26.626124509021842	33.0	25.0	35.0	2.0	35.0
98-99	26.561379703137987	33.0	25.0	35.0	2.0	35.0
100-101	26.38714473047243	33.0	24.5	35.0	2.0	35.0
102-103	25.03513106479436	31.0	21.5	34.0	2.0	35.0
104-105	26.067071019327262	33.0	24.0	35.0	2.0	35.0
106-107	26.245682673828405	33.0	24.0	35.0	2.0	35.0
108-109	26.242462711648642	33.0	24.0	35.0	2.0	35.0
110-111	26.141116934842678	33.0	24.0	35.0	2.0	35.0
112-113	26.047447089766465	33.0	24.0	35.0	2.0	35.0
114-115	25.920067852664044	33.0	23.0	35.0	2.0	35.0
116-117	25.785334019318892	33.0	23.0	35.0	2.0	35.0
118-119	25.589316949554956	33.0	19.5	35.0	2.0	35.0
120-121	25.374537763637157	33.0	18.5	35.0	2.0	35.0
122-123	25.150136793331725	33.0	10.0	35.0	2.0	35.0
124-125	23.98909353439523	31.5	2.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	139177.0
3	20288.0
4	12680.0
5	11424.0
6	12692.0
7	14099.0
8	14918.0
9	15890.0
10	16540.0
11	17583.0
12	17494.0
13	17634.0
14	18047.0
15	16283.0
16	14187.0
17	12648.0
18	11774.0
19	11204.0
20	11289.0
21	12222.0
22	14742.0
23	19337.0
24	26079.0
25	33377.0
26	38503.0
27	43476.0
28	51073.0
29	59416.0
30	68938.0
31	81923.0
32	99640.0
33	122557.0
34	157078.0
35	214596.0
36	324364.0
37	388946.0
38	107967.0
39	128.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.622681375432656	19.228693109837643	13.268690554090407	24.87993496063929
2	15.49115141913447	20.065741503983393	39.45322911852547	24.989877958356665
3	19.08552465487319	24.35553491626327	29.09894949624902	27.45999093261452
4	12.84002703386194	16.01320147716969	36.22326022383121	34.92351126513716
5	13.882959859553592	38.01185512169011	33.917234421704876	14.187950597051419
6	33.25029745346357	35.85484887690118	16.955486160760106	13.939367508875147
7	28.5868652597989	32.4323752853396	20.716558110604723	18.26420134425678
8	26.22890158743584	35.753516622350965	19.389350841117214	18.62823094909598
9	26.348683341115525	16.441849401358507	19.006933493088532	38.20253376443743
10-11	25.105012148172683	26.540723304690623	27.310581123384953	21.04368342375174
12-13	25.594650867899194	23.822685882979254	27.195386181937366	23.38727706718419
14-15	22.8437605168984	25.213597655843916	24.732419269865154	27.210222557392534
16-17	23.153209742654994	28.213464823525165	25.426397531123058	23.20692790269679
18-19	23.229890763553904	27.312349105568508	26.858889452223206	22.598870678654382
20-21	24.240984596246424	26.48416157939958	26.46581460036293	22.809039223991064
22-23	23.722449932494488	26.24226659941811	26.316533674568372	23.71874979351903
24-25	23.400572954074473	26.36139363469035	26.69551039142758	23.542523019807597
26-27	23.16867589820755	26.737124854857836	26.4394622824189	23.654736964515713
28-29	23.216326548591677	26.933365049054753	26.486856146077326	23.363452256276243
30-31	23.60411703762175	26.67105986496201	26.298225335832992	23.426597761583242
32-33	23.11056275336279	26.61474936492743	26.378229707961324	23.896458173748456
34-35	23.528940154995052	26.733683615831655	26.30334493722117	23.434031291952127
36-37	23.669934805357684	26.370405742432375	26.36201385124591	23.597645600964032
38-39	23.312449563559607	26.69424592896435	26.284631554094297	23.70867295338175
40-41	23.644497910308907	26.540816811970092	26.38878121321897	23.425904064502035
42-43	23.595900827494738	26.43102452222096	26.30896293684315	23.66411171344116
44-45	23.452355571687328	26.666180657847445	26.285970525840984	23.595493244624244
46-47	23.56622709636659	26.773115770890644	26.035745275535305	23.624911857207465
48-49	23.38441878633157	26.715475471780397	26.28986492305246	23.610240818835578
50-51	23.299385395257392	26.872167808262176	26.1563734377447	23.67207335873573
52-53	23.615850690582484	26.678750850701594	26.045344322158765	23.66005413655715
54-55	23.579548332732067	26.578432577412407	26.133540010157645	23.708479079697877
56-57	23.403875281113923	26.843029244657817	26.06283693201002	23.69025854221824
58-59	23.40879075762555	26.601054771094763	26.12320522839424	23.86694924288545
60-61	23.366731463090925	26.66117372709825	26.298252082317447	23.673842727493373
62-63	23.2518184515425	26.740747609740907	26.413434041360013	23.59399989735658
64-65	23.237758882956907	26.6822408703463	26.311446775386532	23.768553471310263
66-67	23.275154440524346	26.833194542973	26.04787089126757	23.843780125235085
68-69	23.57467334027124	26.520970646051172	26.26252815082787	23.641827862849716
70-71	23.347880422836184	26.362572733612062	26.272444505724202	24.017102337827556
72-73	23.47259762976166	26.45771992702011	26.25960900737266	23.810073435845567
74-75	23.29453513411247	26.704616910088713	26.15018596661785	23.850661989180967
76-77	23.403707983012822	26.430534454711363	26.318957546594262	23.846800015681556
78-79	23.530077680209498	26.6005492896016	26.25036065024965	23.619012379939257
80-81	23.439406216803814	26.553314437134752	26.16703555294287	23.840243793118564
82-83	23.426715199953133	26.58609207272769	26.261031819079818	23.72616090823936
84-85	23.566245880308028	26.580349520789852	26.002579515230856	23.85082508367126
86-87	23.362789051257444	26.503075349645766	26.176167323338934	23.957968275757857
88-89	23.399588496762195	26.464146756273532	26.15877893395906	23.977485813005213
90-91	23.292142495470124	26.61970469193207	26.26086052562202	23.827292286975783
92-93	23.318760094862245	26.427862029045006	26.349184602748384	23.904193273344365
94-95	23.146493773776736	26.502769070286636	26.456625240740838	23.89411191519579
96-97	23.370685398202994	26.290454519624813	26.414501317336462	23.92435876483573
98-99	23.119098506379206	26.4539985234703	26.223026463006587	24.2038765071439
100-101	23.070221484823044	26.476960155112288	26.443852087339216	24.008966272725452
102-103	22.936101116111548	26.284589942661402	26.60500505721525	24.1743038840118
104-105	22.942306023403226	26.43684287733769	26.36349579674973	24.25735530250935
106-107	22.86841385861143	26.661114034772755	26.263038349947536	24.20743375666828
108-109	22.80576007048652	26.568903328836146	26.321363473663922	24.30397312701341
110-111	22.74514002336807	26.597324655779193	26.307096244367255	24.350439076485486
112-113	22.84125826579115	26.82892502151314	26.24512444141279	24.08469227128292
114-115	22.802314052717986	26.7612183161991	26.230923746530916	24.205543884552007
116-117	22.78621169057491	26.74329579554445	26.381786801100233	24.08870571278041
118-119	22.743513501607858	26.68778908418131	26.17649002246597	24.392207391744858
120-121	22.625210183243226	26.895481715544594	26.26879755141202	24.21051054980016
122-123	22.70647097110271	26.729580874198383	26.2250924825635	24.33885567213541
124-125	22.697943181595313	26.753315552836742	26.237662053477674	24.311079212090267
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	815.0
1	651.5
2	383.0
3	247.5
4	199.0
5	182.5
6	182.5
7	197.0
8	240.0
9	273.0
10	277.5
11	306.0
12	359.0
13	395.5
14	431.5
15	515.0
16	618.0
17	754.0
18	860.0
19	1027.5
20	1313.5
21	1658.5
22	2257.0
23	3099.5
24	4113.5
25	5516.5
26	7928.5
27	11242.5
28	13799.0
29	16384.5
30	19459.5
31	23740.0
32	29248.0
33	33042.5
34	36216.5
35	40049.5
36	44991.0
37	49752.0
38	53242.5
39	56729.0
40	61425.0
41	66721.5
42	69875.0
43	72885.5
44	76139.0
45	79234.0
46	82785.5
47	85864.0
48	88292.0
49	89799.0
50	91334.5
51	91526.0
52	89016.5
53	87961.0
54	87175.5
55	82175.5
56	75570.0
57	68888.0
58	63686.0
59	58098.0
60	49899.5
61	42020.5
62	34327.5
63	25838.0
64	19550.0
65	15819.5
66	12308.5
67	9524.0
68	7290.0
69	5621.5
70	4440.5
71	3222.0
72	2397.5
73	1974.5
74	1462.0
75	1033.5
76	826.5
77	604.5
78	405.0
79	259.0
80	176.0
81	123.0
82	83.0
83	54.5
84	36.5
85	33.0
86	28.5
87	18.5
88	10.0
89	8.5
90	10.5
91	11.5
92	8.5
93	5.5
94	4.0
95	4.5
96	5.5
97	5.0
98	2.0
99	3.5
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03603186132754944
2	0.01823617431492111
3	0.023786314323810145
4	0.020570757017072845
5	0.013567008910617639
6	0.004845360325220585
7	0.0048894090554498625
8	0.0060346760414110925
9	0.0032155573067372974
10-11	0.005219774532169449
12-13	0.007157918662257682
14-15	0.0025107776230688487
16-17	0.0013655106371076194
18-19	0.0
20-21	0.0037221177043739945
22-23	0.0012333644464197853
24-25	0.0021363634161199853
26-27	0.0011012182557319512
28-29	0.002004217225432151
30-31	0.0016298030184832876
32-33	0.0
34-35	0.006188846597213565
36-37	0.007246016122716238
38-39	0.0012774131766490632
40-41	9.25023334814839E-4
42-43	0.002092314685890707
44-45	9.25023334814839E-4
46-47	0.020196342810123984
48-49	0.002753045639329878
50-51	0.009580598824867975
52-53	0.0012333644464197853
54-55	8.809746045855608E-5
56-57	1.5417055580247316E-4
58-59	0.0064751633437038734
60-61	0.0011452669859612294
62-63	0.009624647555097253
64-65	0.00969072065044117
66-67	1.1012182557319512E-4
68-69	0.003325679132310492
70-71	0.0050215552461376975
72-73	0.012003278987478268
74-75	0.002092314685890707
76-77	0.0013214619068783412
78-79	3.5238984183422434E-4
80-81	0.004030458815978941
82-83	8.149015092416438E-4
84-85	8.369258743562829E-4
86-87	0.013589033275732278
88-89	0.0
90-91	3.303654767195853E-4
92-93	0.0081269907273018
94-95	0.0060346760414110925
96-97	0.00449297048338636
98-99	0.0011012182557319512
100-101	0.016452200740635352
102-103	0.00832521001333355
104-105	0.00810496636218716
106-107	0.018434393600952864
108-109	0.012135425178166102
110-111	0.019777879872945842
112-113	0.003898312625291107
114-115	0.01508669010352773
116-117	0.006387065883245316
118-119	0.00497750651590842
120-121	0.007488284138977268
122-123	0.008391283108677467
124-125	0.054950790961024364
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2270213.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.30942011073986
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.13713565889724	41.557914020756
2	13.014164915055673	14.39611829354531
3	4.959546424393869	8.229289102365547
4	2.343341311764874	5.184353963010226
5	1.2651946688897993	3.4988591731747167
6	0.7995821904716054	2.653465637151579
7	0.5255272757032182	2.0346626210065337
8	0.3554647504491333	1.5728439373720335
9	0.25506361146543866	1.2696678397354026
>10	1.2279858425809456	11.948143227089968
>50	0.07884061313210476	3.032094220488232
>100	0.035660502632563956	3.491199586994751
>500	0.0017686825934283609	0.667743250356108
>1k	7.235519700387923E-4	0.4636451269536202
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.404873022927805E-5
2	0.0	0.0	0.0	0.0	4.404873022927805E-5
3	0.0	0.0	0.0	0.0	4.404873022927805E-5
4	0.0	0.0	0.0	0.0	4.404873022927805E-5
5	0.0	0.0	0.0	0.0	4.404873022927805E-5
6	0.0	0.0	0.0	0.0	8.80974604585561E-5
7	0.0	0.0	0.0	0.0	8.80974604585561E-5
8	0.0	0.0	0.0	0.0	8.80974604585561E-5
9	0.0	0.0	0.0	0.0	8.80974604585561E-5
10-11	0.0	0.0	0.0	0.0	8.80974604585561E-5
12-13	0.0	0.0	0.0	0.0	2.863167464903073E-4
14-15	0.0	0.0	0.0	0.0	3.3036547671958536E-4
16-17	0.0	0.0	0.0	4.404873022927805E-5	4.404873022927805E-4
18-19	0.0	0.0	0.0	4.404873022927805E-5	4.404873022927805E-4
20-21	0.0	0.0	0.0	1.1012182557319512E-4	4.404873022927805E-4
22-23	0.0	0.0	0.0	3.5238984183422434E-4	4.404873022927805E-4
24-25	0.0	0.0	0.0	8.589502394709219E-4	5.285847627513365E-4
26-27	0.0	0.0	0.0	0.0016518273835979267	5.726334929806146E-4
28-29	0.0	0.0	0.0	0.0035459227834568827	5.726334929806146E-4
30-31	0.0	0.0	0.0	0.010263354143421784	5.726334929806146E-4
32-33	0.0	0.0	0.0	0.025151824960917765	6.607309534391707E-4
34-35	0.0	0.0	0.0	0.04622914237562731	7.047796836684488E-4
36-37	0.0	0.0	0.0	0.07871508091971988	7.047796836684488E-4
38-39	0.0	0.0	0.0	0.1291288526671286	7.928771441270048E-4
40-41	0.0	0.0	0.0	0.1841457167234969	8.369258743562829E-4
42-43	0.0	0.0	0.0	0.23892031276360412	8.80974604585561E-4
44-45	0.0	0.0	0.0	0.30276894723094266	9.69072065044117E-4
46-47	0.0	0.0	0.0	0.3807351997367648	0.001013120795273395
48-49	0.0	0.0	0.0	0.46114615677031184	0.001079193890617312
50-51	0.0	0.0	0.0	0.5445083787292206	0.0011452669859612291
52-53	0.0	0.0	0.0	0.6324516686319742	0.0012553888115344244
54-55	0.0	0.0	0.0	0.7252843675901777	0.0013214619068783414
56-57	0.0	0.0	0.0	0.8300983211707448	0.0013875350022222585
58-59	0.0	0.0	0.0	0.9490959658851394	0.0014315837324515366
60-61	0.0	0.0	0.0	1.0722341912410862	0.0014536080975661755
62-63	0.0	0.0	0.0	1.199446924143241	0.0014536080975661755
64-65	0.0	0.0	0.0	1.3358438172982008	0.0014536080975661755
66-67	0.0	0.0	0.0	1.4976568277954536	0.0015417055580247314
68-69	0.0	0.0	0.0	1.6780584024494618	0.0015857542882540096
70-71	0.0	0.0	0.0	1.8721811565698903	0.0016298030184832878
72-73	0.0	0.0	0.0	2.06835217664598	0.0017839735742857608
74-75	0.0	0.0	0.0	2.2711965793518054	0.001828022304515039
76-77	0.0	0.0	0.0	2.50793647996906	0.0018500466696296778
78-79	0.0	0.0	0.0	2.768418646179896	0.001894095399858956
80-81	0.0	0.0	0.0	3.0418511390781395	0.0019821928603175124
82-83	0.0	0.0	0.0	3.3271988135034025	0.0022024365114639024
84-85	0.0	0.0	0.0	3.6235806948511	0.0022244608765785412
86-87	0.0	0.0	0.0	3.9532193675219025	0.0023566070672663753
88-89	0.0	0.0	0.0	4.310873032618526	0.0024667288928395706
90-91	0.0	0.0	0.0	4.6925552800552195	0.0024667288928395706
92-93	0.0	0.0	0.0	5.087958706958334	0.0025548263532981265
94-95	0.0	0.0	0.0	5.499858383332313	0.0025988750835274047
96-97	0.0	0.0	0.0	5.928188236081812	0.0027310212742152388
98-99	0.0	0.0	0.0	6.399707868821119	0.002863167464903073
100-101	0.0	0.0	0.0	6.908801068446	0.002863167464903073
102-103	0.0	0.0	0.0	7.427695110546896	0.0029953136555909074
104-105	0.0	0.0	0.0	7.949188027731319	0.003039362385820185
106-107	4.404873022927805E-5	0.0	0.0	8.489798093835248	0.003039362385820185
108-109	4.404873022927805E-5	0.0	0.0	9.060647613241578	0.003061386750934824
110-111	4.404873022927805E-5	0.0	0.0	9.666868263022016	0.0031494842113933804
112-113	4.404873022927805E-5	0.0	0.0	10.295421619028698	0.0032155573067372974
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	5890	0.0	35.666897	1
TCAACGC	6910	0.0	30.309225	4
ATCAACG	7000	0.0	29.920853	3
TATCAAC	6960	0.0	29.83634	2
GGTATCA	3800	0.0	29.756092	1
CAACGCA	7045	0.0	29.55886	5
AACGCAG	7105	0.0	29.30989	6
CATGGGG	6145	0.0	27.401527	4
TACATGG	10170	0.0	26.679228	2
ACATGGG	9910	0.0	26.538597	3
GTACATG	10805	0.0	26.4927	1
ACGCAGA	8085	0.0	25.683584	7
CGCAGAG	8285	0.0	24.632147	8
GCGTAAG	200	1.8239043E-6	23.804874	1
GCAGAGT	9555	0.0	20.237335	9
AGAGTAC	7710	0.0	20.02507	10-11
GTATAAT	1585	0.0	19.899975	1
ATGGGGA	3665	0.0	19.80553	5
GTGTAGC	1775	0.0	19.781515	1
GTGTAGG	995	0.0	19.139597	1
>>END_MODULE