FastQCFastQC Report
Mon 15 Aug 2016
SRR2921008_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921008_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2270213
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35940.1583111364440253No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29640.13056043639958012No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23790.10479192921545248No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA79950.040.7023471
GGTATCA51200.040.276471
TATCAAC98950.032.446592
GTACCGT2400.032.2249376
TCAACGC100550.031.7595714
ATCAACG101900.031.6304553
CAACGCA102400.031.2607735
AACGCAG106350.030.0397786
ACGCAGA121900.025.7197557
CGCAGAG122350.025.6268548
TACATGG107350.024.6363892
GTAAGGT9250.024.4756244
GTACATG112400.024.2853531
ACATGGG102250.024.1223353
CATGGGG61350.023.0157814
TATACCG1300.002052761622.8846365
TAAGGTG9900.022.8384065
GAGTACT66800.022.22491612-13
AGAGTAC122550.022.09051510-11
GCAGAGT137950.022.039289
Produced by FastQC (version 0.11.5)