FastQCFastQC Report
Mon 15 Aug 2016
SRR2921007_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921007_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3963223
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA71700.038.4284671
TCAACGC79100.033.92334
CAACGCA82750.032.2819675
TATCAAC86900.031.0853712
GGTATCA48650.030.8254151
GTACATG157750.030.8208031
ATCAACG87250.030.7557013
AACGCAG88950.030.165246
TACATGG160100.029.5086382
ACATGGG155400.029.3669513
CATGGGG116250.029.1217464
ACGCAGA100350.026.3233877
CGCAGAG107200.024.5303448
GCAGAGT120900.021.0124999
ATGGGGA62650.020.322375
ATGGGGG63050.019.627275
AGAGTAC102800.019.59119610-11
TATACTG18700.018.7712025
GAGTACA112400.017.577741
CAGAGTA112700.017.18392810-11
Produced by FastQC (version 0.11.5)