##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921007_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3963223
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.467382733699313	31.0	28.0	33.0	16.0	34.0
2	28.528994457288928	31.0	28.0	34.0	16.0	34.0
3	28.642024180824546	31.0	28.0	34.0	16.0	34.0
4	32.1264301806888	35.0	32.0	37.0	20.0	37.0
5	32.175747112892715	35.0	33.0	37.0	22.0	37.0
6	32.11772539672887	35.0	32.0	37.0	21.0	37.0
7	32.01594636486516	35.0	32.0	37.0	19.0	37.0
8	32.01418391041837	35.0	32.0	37.0	19.0	37.0
9	33.19920958270579	37.0	33.0	39.0	18.0	39.0
10-11	33.132007838065135	37.0	33.0	39.0	17.5	39.0
12-13	33.04838246548327	37.0	33.0	39.0	17.0	39.0
14-15	33.94649026814792	38.0	33.0	41.0	17.0	41.0
16-17	33.79692121790775	38.0	32.5	41.0	17.0	41.0
18-19	33.87332683525504	38.0	33.0	41.0	17.5	41.0
20-21	33.88941260686063	38.0	33.0	40.5	18.0	41.0
22-23	33.82700027225316	38.0	32.5	40.0	17.5	41.0
24-25	33.73656529042146	38.0	32.5	40.5	17.0	41.0
26-27	33.588916646880584	38.0	32.0	40.0	16.0	41.0
28-29	33.5094693132332	38.0	32.0	40.0	16.0	41.0
30-31	33.387242781947926	38.0	32.0	40.0	15.0	41.0
32-33	33.30621226713713	38.0	32.0	40.0	12.5	41.0
34-35	33.141968544288325	38.0	32.0	40.0	9.0	41.0
36-37	32.98650631064666	38.0	31.5	40.0	9.0	41.0
38-39	32.852449004257394	38.0	31.0	40.0	9.0	41.0
40-41	32.76606413517483	38.0	31.0	40.0	8.0	41.0
42-43	32.54710736690819	38.0	31.0	40.0	7.5	41.0
44-45	32.3645001807872	38.0	31.0	40.0	4.0	41.0
46-47	32.16940833256166	37.0	30.5	40.0	2.0	41.0
48-49	31.997936780241737	37.0	30.0	40.0	2.0	41.0
50-51	31.756453396642076	37.0	30.0	40.0	2.0	41.0
52-53	31.557477966796217	37.0	30.0	40.0	2.0	41.0
54-55	31.329156724211582	36.0	29.5	40.0	2.0	41.0
56-57	31.103309982809446	36.0	29.0	39.5	2.0	41.0
58-59	30.824603737917347	35.5	29.0	39.0	2.0	41.0
60-61	30.60866307548175	35.0	29.0	39.0	2.0	40.5
62-63	30.474297560344194	35.0	29.0	39.0	2.0	40.5
64-65	30.22560236958657	35.0	29.0	39.0	2.0	40.0
66-67	29.94954523124235	35.0	29.0	38.0	2.0	40.0
68-69	29.631007011212844	34.5	28.0	37.5	2.0	40.0
70-71	29.304022382792994	34.0	28.0	37.0	2.0	39.5
72-73	29.023090171812186	34.0	28.0	36.0	2.0	39.0
74-75	28.729200829728732	34.0	28.0	36.0	2.0	39.0
76-77	28.461880393810794	34.0	27.5	35.5	2.0	37.5
78-79	28.161574052229714	34.0	27.0	35.0	2.0	37.0
80-81	27.862552523539556	34.0	26.0	35.0	2.0	37.0
82-83	27.618219943717524	33.5	26.0	35.0	2.0	36.0
84-85	27.39714898707441	33.0	26.0	35.0	2.0	36.0
86-87	27.348365459122537	33.0	26.0	35.0	2.0	35.5
88-89	27.464342909798415	34.0	27.0	35.0	2.0	35.0
90-91	27.38946092611998	34.0	26.5	35.0	2.0	35.0
92-93	27.245073012545596	34.0	26.0	35.0	2.0	35.0
94-95	27.16813172006723	34.0	26.0	35.0	2.0	35.0
96-97	27.07647437451791	34.0	26.0	35.0	2.0	35.0
98-99	27.014039835759938	34.0	25.5	35.0	2.0	35.0
100-101	26.843709147832456	33.5	25.0	35.0	2.0	35.0
102-103	25.470728495469473	31.5	23.5	34.0	2.0	35.0
104-105	26.506948763670376	33.0	25.0	35.0	2.0	35.0
106-107	26.703827162892424	33.0	25.0	35.0	2.0	35.0
108-109	26.695017035377518	34.0	25.0	35.0	2.0	35.0
110-111	26.580894640548866	33.5	25.0	35.0	2.0	35.0
112-113	26.480161600798137	33.0	25.0	35.0	2.0	35.0
114-115	26.339666730840026	33.0	24.5	35.0	2.0	35.0
116-117	26.201527771715092	33.0	24.0	35.0	2.0	35.0
118-119	26.008637540708662	33.0	23.5	35.0	2.0	35.0
120-121	25.78060482592072	33.0	23.0	35.0	2.0	35.0
122-123	25.545252941860703	33.0	19.5	35.0	2.0	35.0
124-125	24.354193670151794	32.0	10.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	222115.0
3	33208.0
4	21450.0
5	18710.0
6	20198.0
7	23206.0
8	25012.0
9	26064.0
10	27491.0
11	28240.0
12	28104.0
13	27878.0
14	27425.0
15	24949.0
16	21751.0
17	19501.0
18	18835.0
19	18218.0
20	18458.0
21	20870.0
22	24917.0
23	33537.0
24	44767.0
25	56982.0
26	66738.0
27	77237.0
28	90855.0
29	105297.0
30	123159.0
31	146620.0
32	179075.0
33	221058.0
34	285358.0
35	390628.0
36	600983.0
37	682451.0
38	161757.0
39	121.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.38896278777446	17.80376438399039	12.233263585125206	25.574009243109945
2	16.350321527057307	19.303610829232174	38.25681129853221	26.089256345178306
3	19.79714929853018	22.99305230795167	28.440059025980467	28.769739367537678
4	13.51616955004988	14.846468708366636	34.59431453075232	37.04304721083116
5	14.75857369975017	36.82267141090671	33.44605950488304	14.972695384460092
6	35.28081382072074	34.092986814398564	16.17717229345691	14.449027071423796
7	30.09612606531627	31.01340516398458	20.08990606922742	18.80056270147173
8	27.448900852943563	35.02927845748479	18.72075119190549	18.801069497666152
9	26.66877443467492	15.586036045356208	18.375405425727926	39.36978409424095
10-11	26.252640506220192	25.350512494453408	26.667261331230225	21.729585668096174
12-13	26.326346634871665	22.49448161220827	26.947560333216625	24.231611419703437
14-15	23.600889955950084	23.981234470085735	23.82963664337482	28.588238930589366
16-17	24.26375390444527	27.04659294175712	24.64393502982884	24.045718123968772
18-19	24.334272888505136	26.36660869196611	25.790802082042823	23.50831633748593
20-21	25.004504113504673	25.55177282916869	25.608875399818924	23.834847657507712
22-23	24.716642687690786	25.155808673509295	25.597422180191025	24.53012645860889
24-25	24.37433678481732	25.474856078126262	25.58983966010076	24.560967476955657
26-27	24.20583614800505	25.691493307616494	25.60485793919098	24.497812605187473
28-29	24.27449251694638	25.792538545699717	25.650490223785088	24.282478713568814
30-31	24.486066178414028	25.71395625306493	25.438773227714456	24.361204340806584
32-33	24.23873501929301	25.709886336182237	25.390487160385266	24.660891484139487
34-35	24.382064136107324	25.727343508208506	25.5753348247377	24.31525753094647
36-37	24.560314449625466	25.501503270175675	25.52907173997167	24.409110540227193
38-39	24.46810738605239	25.64193109587579	25.49442250487553	24.39553901319629
40-41	24.49229340691089	25.610389675928204	25.56546346165249	24.331853455508412
42-43	24.490868569345064	25.543378632496427	25.444453486193563	24.52129931196495
44-45	24.368191763736515	25.599126052803456	25.507608369414527	24.525073814045502
46-47	24.483267334616336	25.61748791649952	25.499842327401506	24.39940242148264
48-49	24.40235261403943	25.78799171654579	25.30903355243054	24.500622116984236
50-51	24.350859546232872	25.934869375746878	25.34824533257696	24.366025745443295
52-53	24.515759416432203	25.687785072361212	25.357869779216646	24.43858573198994
54-55	24.391926756433854	25.650374758876314	25.403390933875652	24.554307550814176
56-57	24.332217529197603	25.829879588782884	25.38170758277916	24.456195299240353
58-59	24.407538091297994	25.586153417952907	25.409200252440566	24.597108238308536
60-61	24.31652452563585	25.66863897860315	25.571142006459425	24.443694489301574
62-63	24.23042812334751	25.707374245524882	25.5704372558448	24.491760375282805
64-65	24.176359376719127	25.682653455712313	25.521074641690213	24.619912525878348
66-67	24.203131471621596	25.72103996841959	25.45474111747365	24.621087442485162
68-69	24.337539376456586	25.525620813176186	25.51296634795414	24.623873462413084
70-71	24.071948623698567	25.530113655217036	25.623389081160724	24.774548639923673
72-73	24.198242341646782	25.545285802122308	25.52750758917717	24.72896426705374
74-75	24.03071405690922	25.721483476534306	25.534459890543765	24.71334257601271
76-77	24.236332919396336	25.39572238188003	25.56634365450731	24.80160104421632
78-79	24.12360874682406	25.52366478741855	25.591198624040658	24.761527841716735
80-81	24.145037216398126	25.56131180170364	25.581283853447783	24.712367128450445
82-83	24.145494238363465	25.477299832065555	25.679790096447185	24.6974158331238
84-85	24.023047236236692	25.643128661057514	25.517143495161065	24.81668060754473
86-87	24.057559817348373	25.55006775179739	25.65716846065822	24.735203970196025
88-89	23.935178515062113	25.43402175451646	25.62172252230066	25.009077208120768
90-91	23.884820201882572	25.503766531800732	25.67533265458803	24.936080611728666
92-93	23.966439542966704	25.427647014366162	25.814089138828837	24.791824303838293
94-95	23.925059530185617	25.329004186448557	25.84104011564242	24.904896167723404
96-97	23.959100717423034	25.068145915086877	25.89264682708456	25.080106540405527
98-99	23.86892139714632	25.299697413480743	25.76816149924656	25.063219690126378
100-101	23.823437484424403	25.19266422941816	25.911763158296523	25.07213512786091
102-103	23.597230754517323	25.103065708829007	26.043857700117172	25.255845836536494
104-105	23.59198986905192	25.32494773722267	25.848729966250783	25.23433242747463
106-107	23.561762344759117	25.294999004409117	25.859053605692004	25.28418504513976
108-109	23.484148029114856	25.274629881790293	25.924286133903706	25.316935955191145
110-111	23.424763963656567	25.400414121207614	25.917213867016088	25.257608048119728
112-113	23.470719682996112	25.40680213953399	25.92736606086236	25.195112116607532
114-115	23.446248019131914	25.509101551799347	25.81824252222692	25.226407906841818
116-117	23.25839189434365	25.43330605574973	25.92454228038076	25.383759769525867
118-119	23.371119541316954	25.373063524129964	25.77910713051079	25.47670980404229
120-121	23.241328650541348	25.465343621479136	25.903535258970876	25.389792469008636
122-123	23.20631908933206	25.48031410226404	25.809950642839198	25.5034161655647
124-125	23.197797844953925	25.55308229250531	25.743133917979392	25.505985944561377
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	663.0
1	571.5
2	401.5
3	314.5
4	266.5
5	210.0
6	209.0
7	256.5
8	330.5
9	378.0
10	386.0
11	405.5
12	430.5
13	458.5
14	530.0
15	633.5
16	719.5
17	791.5
18	919.0
19	1108.0
20	1339.0
21	1669.5
22	2135.5
23	2736.5
24	3586.5
25	4800.5
26	6429.0
27	8758.0
28	11637.0
29	15304.5
30	20201.0
31	25758.0
32	31767.5
33	36416.5
34	40645.5
35	46589.0
36	53985.5
37	62263.5
38	71046.5
39	79166.5
40	87045.5
41	96086.5
42	104925.5
43	115140.0
44	126116.0
45	136584.0
46	147841.5
47	156814.0
48	164522.0
49	171302.5
50	175891.5
51	180627.0
52	182467.5
53	183114.5
54	182414.5
55	174941.0
56	163155.5
57	149363.0
58	136833.5
59	122651.5
60	104950.5
61	88669.5
62	72125.5
63	54360.5
64	39225.0
65	28746.5
66	21353.0
67	16049.5
68	11933.0
69	8814.5
70	6577.0
71	4842.5
72	3469.5
73	2516.5
74	1856.0
75	1302.5
76	863.5
77	543.0
78	326.5
79	230.5
80	160.5
81	111.0
82	85.0
83	61.5
84	52.0
85	47.5
86	35.0
87	24.5
88	19.5
89	17.0
90	17.5
91	16.0
92	14.5
93	11.5
94	8.5
95	6.5
96	12.5
97	12.5
98	7.0
99	7.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03413888141040764
2	0.018318424171438245
3	0.02270879029517138
4	0.02033698330878681
5	0.013196330360416255
6	0.004743613972769133
7	0.004995933864937703
8	0.0060809094012625586
9	0.002649358867769994
10-11	0.005159941794847274
12-13	0.008465332382255553
14-15	0.0026619748623784227
16-17	0.002825982792287994
18-19	0.0
20-21	0.004516526069817419
22-23	7.317276872888555E-4
24-25	0.0012237514770175688
26-27	0.0015391513422282823
28-29	0.0034063185442757067
30-31	0.0010723595417164264
32-33	1.2615994608428544E-5
34-35	0.007355124856713842
36-37	0.009146596091110694
38-39	0.0016148473098788537
40-41	0.001463455374577711
42-43	0.003166614646715565
44-45	0.0013751434123187114
46-47	0.022670942311346094
48-49	0.0035829424687937065
50-51	0.011695027002013261
52-53	0.002132103088824424
54-55	2.018559137348567E-4
56-57	3.1539986521071363E-4
58-59	0.007821916657225697
60-61	5.803357519877131E-4
62-63	0.011392243131410976
64-65	0.011379627136802547
66-67	1.6400792990957106E-4
68-69	0.004920237897287132
70-71	0.005765509536051845
72-73	0.01280523452755497
74-75	0.002182567067258138
76-77	0.0015013033584029968
78-79	6.181837358129987E-4
80-81	0.004667918005118562
82-83	0.0017536232505715676
84-85	0.001463455374577711
86-87	0.015076113557072111
88-89	0.0
90-91	7.064956980719986E-4
92-93	0.010206339638218691
94-95	0.008011156576352126
96-97	0.0055384216331001304
98-99	0.0014886873637945683
100-101	0.017523616511107246
102-103	0.008541028349906124
104-105	0.007985924587135268
106-107	0.018974455891076533
108-109	0.010975915309332833
110-111	0.021611198764238098
112-113	0.002952142738372279
114-115	0.015883537212011536
116-117	0.0066107811748165575
118-119	0.005677197573792845
120-121	0.008705036279815696
122-123	0.009424147972496123
124-125	0.055358984341784455
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	3963223.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.693851660128765
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.42030926128096	37.219582665321546
2	13.157025825433543	13.339606309660226
3	5.041965845503003	7.667900059420946
4	2.538327612686624	5.147104138493779
5	1.4752131656822218	3.7392118694081753
6	0.9218079750508997	2.803799804781239
7	0.6413010556930552	2.27570144107514
8	0.46031724150085085	1.8668203165795039
9	0.34906213336034786	1.5925773607863802
>10	1.8487303997771531	16.806658904692483
>50	0.10713636043735578	3.7076216041766403
>100	0.0369961387274374	3.1292172999637735
>500	0.0013552386747336573	0.45259527020376317
>1k	4.517461918762003E-4	0.25160295543648015
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.046397843371418E-5
2	0.0	0.0	0.0	2.523198921685709E-5	7.569596765057127E-5
3	0.0	0.0	0.0	7.569596765057127E-5	7.569596765057127E-5
4	0.0	0.0	0.0	7.569596765057127E-5	7.569596765057127E-5
5	0.0	0.0	0.0	7.569596765057127E-5	7.569596765057127E-5
6	0.0	0.0	0.0	7.569596765057127E-5	1.0092795686742836E-4
7	0.0	0.0	0.0	1.0092795686742836E-4	1.0092795686742836E-4
8	0.0	0.0	0.0	1.0092795686742836E-4	1.0092795686742836E-4
9	0.0	0.0	0.0	1.2615994608428544E-4	1.5139193530114254E-4
10-11	0.0	0.0	0.0	1.640079299095711E-4	1.766239245179996E-4
12-13	0.0	0.0	0.0	2.2708790295171378E-4	4.1632782207814197E-4
14-15	0.0	0.0	0.0	2.523198921685709E-4	4.920237897287133E-4
16-17	0.0	0.0	0.0	2.523198921685709E-4	5.298717735539989E-4
18-19	0.0	0.0	0.0	2.523198921685709E-4	6.560317196382842E-4
20-21	0.0	0.0	0.0	2.9016787599385653E-4	7.44343681897284E-4
22-23	2.523198921685709E-5	0.0	0.0	4.5417580590342756E-4	8.200396495478554E-4
24-25	2.523198921685709E-5	0.0	0.0	8.200396495478554E-4	8.452716387647124E-4
26-27	2.523198921685709E-5	0.0	0.0	0.0012363674716259973	9.083516118068551E-4
28-29	2.523198921685709E-5	0.0	0.0	0.0037217184094864206	9.083516118068551E-4
30-31	2.523198921685709E-5	0.0	0.0	0.013032322430506687	9.209676064152836E-4
32-33	2.523198921685709E-5	0.0	0.0	0.03205724230001693	9.840475794574265E-4
34-35	2.523198921685709E-5	0.0	0.0	0.05530852036335074	0.0010723595417164262
36-37	2.523198921685709E-5	0.0	0.0	0.09378730391905779	0.0010849755363248547
38-39	2.523198921685709E-5	0.0	0.0	0.15618601325234538	0.001160671503975426
40-41	2.523198921685709E-5	0.0	0.0	0.2249305678736725	0.001185903493192283
42-43	2.523198921685709E-5	0.0	0.0	0.2915682513953921	0.0011985194878007116
44-45	2.523198921685709E-5	0.0	0.0	0.3690052263019265	0.0012615994608428543
46-47	2.523198921685709E-5	0.0	0.0	0.4598025394987867	0.0013751434123187114
48-49	3.7847983825285635E-5	0.0	0.0	0.5563653622316989	0.001463455374577711
50-51	5.046397843371418E-5	0.0	0.0	0.6618350771581614	0.0015139193530114253
52-53	5.046397843371418E-5	0.0	0.0	0.7653745449095345	0.001576999326053568
54-55	5.046397843371418E-5	0.0	0.0	0.8764457614421395	0.0016400792990957107
56-57	5.046397843371418E-5	0.0	0.0	1.0074502494560613	0.0016653112883125679
58-59	5.046397843371418E-5	0.0	0.0	1.147033613803715	0.001715775266746282
60-61	5.046397843371418E-5	0.0	0.0	1.3008099720858504	0.001715775266746282
62-63	5.046397843371418E-5	0.0	0.0	1.4583080487774724	0.0017662392451799963
64-65	5.046397843371418E-5	0.0	0.0	1.6186321082613822	0.0018040872290052818
66-67	5.046397843371418E-5	0.0	0.0	1.8044278608597093	0.0018419352128305672
68-69	5.046397843371418E-5	0.0	0.0	2.012162828082094	0.0018671672020474245
70-71	5.046397843371418E-5	0.0	0.0	2.232576869885949	0.0018923991912642817
72-73	5.046397843371418E-5	0.0	0.0	2.458554565312121	0.00216995107264971
74-75	6.307997304214272E-5	0.0	0.0	2.692064009519525	0.0022961110187339952
76-77	7.569596765057127E-5	0.0	0.0	2.9515245546364666	0.0022961110187339952
78-79	7.569596765057127E-5	0.0	0.0	3.2424872382906536	0.0022961110187339952
80-81	7.569596765057127E-5	0.0	0.0	3.5517304981324544	0.002333959002559281
82-83	7.569596765057127E-5	0.0	0.0	3.8773240869867784	0.0024727349432519946
84-85	7.569596765057127E-5	0.0	0.0	4.204570371134807	0.0024727349432519946
86-87	7.569596765057127E-5	0.0	0.0	4.560404499065534	0.002573662900119423
88-89	7.569596765057127E-5	0.0	0.0	4.953077331252872	0.002712438840812137
90-91	7.569596765057127E-5	0.0	0.0	5.37730276595589	0.0027250548354205654
92-93	7.569596765057127E-5	0.0	0.0	5.811103740566706	0.002914294754546994
94-95	7.569596765057127E-5	0.0	0.0	6.2510234725626095	0.0029395267437638505
96-97	7.569596765057127E-5	0.0	0.0	6.709072388810824	0.0032170786251492786
98-99	7.569596765057127E-5	0.0	0.0	7.201827906226826	0.0033558545658419927
100-101	7.569596765057127E-5	0.0	0.0	7.743255930842146	0.0033558545658419927
102-103	7.569596765057127E-5	0.0	0.0	8.303910731240709	0.003406318544275707
104-105	7.569596765057127E-5	0.0	0.0	8.857740278556115	0.003456782522709421
106-107	7.569596765057127E-5	0.0	0.0	9.423542404754919	0.0034693985173178494
108-109	7.569596765057127E-5	0.0	0.0	10.02466931585732	0.003507246501143135
110-111	7.569596765057127E-5	0.0	0.0	10.663139571000674	0.0035577104795768495
112-113	7.569596765057127E-5	0.0	0.0	11.324974648158834	0.0035829424687937065
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	7170	0.0	38.428467	1
TCAACGC	7910	0.0	33.9233	4
CAACGCA	8275	0.0	32.281967	5
TATCAAC	8690	0.0	31.085371	2
GGTATCA	4865	0.0	30.825415	1
GTACATG	15775	0.0	30.820803	1
ATCAACG	8725	0.0	30.755701	3
AACGCAG	8895	0.0	30.16524	6
TACATGG	16010	0.0	29.508638	2
ACATGGG	15540	0.0	29.366951	3
CATGGGG	11625	0.0	29.121746	4
ACGCAGA	10035	0.0	26.323387	7
CGCAGAG	10720	0.0	24.530344	8
GCAGAGT	12090	0.0	21.012499	9
ATGGGGA	6265	0.0	20.32237	5
ATGGGGG	6305	0.0	19.62727	5
AGAGTAC	10280	0.0	19.591196	10-11
TATACTG	1870	0.0	18.771202	5
GAGTACA	11240	0.0	17.57774	1
CAGAGTA	11270	0.0	17.183928	10-11
>>END_MODULE