FastQCFastQC Report
Mon 15 Aug 2016
SRR2921007_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921007_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3963223
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56050.044.013071
GTATCAA85400.043.399971
TCAACGC105750.034.1363754
TATCAAC111800.032.655892
CAACGCA110250.032.5975075
ATCAACG114000.031.8749963
AACGCAG115500.031.1625966
GTACATG149550.026.5764121
ACGCAGA137350.026.0758847
TACATGG156900.024.9014022
ACATGGG150350.024.8817773
CGCAGAG144800.024.6530328
CATGGGG111700.024.3718344
GCAGAGT161150.021.1182969
GGACCGT7650.020.2195846
AGAGTAC142750.020.19384610-11
GTATATA15100.018.9417971
CAGAGTA154300.018.70153210-11
TATACCG3855.0276867E-918.5458415
GAGTACT77200.018.18972812-13
Produced by FastQC (version 0.11.5)