##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921007_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3963223
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.84931683127596	31.0	30.0	34.0	26.0	34.0
2	29.883525352976605	31.0	30.0	34.0	26.0	34.0
3	30.02012200676066	31.0	30.0	34.0	26.0	34.0
4	33.573734054329016	35.0	33.0	37.0	28.0	37.0
5	33.074200215329796	35.0	33.0	37.0	26.0	37.0
6	32.87284641818035	35.0	33.0	37.0	26.0	37.0
7	32.70248280250695	35.0	33.0	37.0	25.0	37.0
8	32.61777422062801	35.0	33.0	37.0	25.0	37.0
9	33.930866620424844	37.0	33.0	39.0	25.0	39.0
10-11	33.824946009851075	37.0	33.0	39.0	25.0	39.0
12-13	33.70595964446109	37.0	33.0	39.0	24.0	39.0
14-15	34.572323712291734	38.0	33.0	41.0	23.0	41.0
16-17	34.546836123024114	38.0	33.0	41.0	23.5	41.0
18-19	34.565624871474554	38.5	33.5	41.0	23.0	41.0
20-21	34.5288941601318	39.0	33.0	41.0	22.5	41.0
22-23	34.49923862472538	39.0	33.0	41.0	23.0	41.0
24-25	34.47114482329155	38.5	33.0	41.0	23.0	41.0
26-27	34.325746999348766	38.0	33.0	41.0	21.5	41.0
28-29	34.202860903865364	38.0	33.0	40.0	20.5	41.0
30-31	34.04816420877654	38.0	33.0	40.0	19.0	41.0
32-33	33.923191427784914	38.0	33.0	40.0	18.5	41.0
34-35	33.806684609975264	38.0	33.0	40.0	18.0	41.0
36-37	33.66808642864659	38.0	33.0	40.0	17.0	41.0
38-39	33.52762713579327	38.0	32.0	40.0	16.0	41.0
40-41	33.33533200125252	38.0	32.0	40.0	15.0	41.0
42-43	33.18702467663313	38.0	32.0	40.0	14.5	41.0
44-45	32.96180015608508	38.0	32.0	40.0	10.0	41.0
46-47	32.826618638416264	38.0	31.5	40.0	9.0	41.0
48-49	32.61706002917323	38.0	31.0	40.0	8.0	41.0
50-51	32.401703865767836	37.0	31.0	40.0	7.0	41.0
52-53	32.14470192568019	37.0	31.0	40.0	4.0	41.0
54-55	31.943849235836588	37.0	30.5	40.0	2.0	41.0
56-57	31.793404635570596	36.0	30.5	40.0	2.0	41.0
58-59	31.705399115820633	36.0	31.0	40.0	2.0	41.0
60-61	31.413652726581372	36.0	30.0	39.0	2.0	41.0
62-63	31.134371949294803	35.0	30.0	39.0	2.0	41.0
64-65	30.85186854234546	35.0	29.5	39.0	2.0	41.0
66-67	30.529837079568825	35.0	29.0	38.5	2.0	40.0
68-69	30.210390507927514	35.0	29.0	37.5	2.0	40.0
70-71	29.854640781000718	35.0	29.0	37.0	2.0	39.5
72-73	29.57604429526171	34.0	29.0	36.5	2.0	39.0
74-75	29.324641585901173	34.0	29.0	36.0	2.0	39.0
76-77	29.01299926852463	34.0	29.0	35.5	2.0	37.5
78-79	28.7135419833807	34.0	28.0	35.0	2.0	37.0
80-81	28.473882494121575	34.0	28.0	35.0	2.0	37.0
82-83	28.244027651232344	34.0	27.5	35.0	2.0	36.0
84-85	28.071789551080016	34.0	27.0	35.0	2.0	36.0
86-87	27.86486654927063	34.0	27.0	35.0	2.0	35.5
88-89	27.755203277736328	34.0	27.0	35.0	2.0	35.0
90-91	27.580713853346126	34.0	26.0	35.0	2.0	35.0
92-93	27.742552967622565	34.0	27.0	35.0	2.0	35.0
94-95	27.698443287193278	34.0	27.0	35.0	2.0	35.0
96-97	27.644323950481716	34.0	27.0	35.0	2.0	35.0
98-99	27.59890876693035	34.0	27.0	35.0	2.0	35.0
100-101	27.469240438905405	34.0	27.0	35.0	2.0	35.0
102-103	26.168990490820224	31.5	24.5	34.0	2.0	35.0
104-105	27.240532516086027	33.5	26.0	35.0	2.0	35.0
106-107	27.442635577155258	34.0	27.0	35.0	2.0	35.0
108-109	27.42751063969905	34.0	27.0	35.0	2.0	35.0
110-111	27.363765046781367	34.0	27.0	35.0	2.0	35.0
112-113	27.26073513905223	34.0	27.0	35.0	2.0	35.0
114-115	27.170220928774384	34.0	27.0	35.0	2.0	35.0
116-117	27.041424618296777	34.0	26.0	35.0	2.0	35.0
118-119	26.91279799294665	34.0	25.0	35.0	2.0	35.0
120-121	26.77310146312736	34.0	25.0	35.0	2.0	35.0
122-123	26.5390509189112	34.0	25.0	35.0	2.0	35.0
124-125	25.419413189719577	32.5	21.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	142144.0
3	44788.0
4	22968.0
5	17554.0
6	19110.0
7	22571.0
8	25142.0
9	26732.0
10	28082.0
11	28598.0
12	29077.0
13	30381.0
14	31939.0
15	26856.0
16	21757.0
17	20105.0
18	18189.0
19	17406.0
20	17635.0
21	18827.0
22	22165.0
23	28978.0
24	39322.0
25	51547.0
26	63242.0
27	74830.0
28	87474.0
29	103289.0
30	121576.0
31	145438.0
32	175054.0
33	216949.0
34	277401.0
35	383420.0
36	613422.0
37	758475.0
38	190501.0
39	279.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.8812551672778	18.6546333520565	12.96576185397194	25.498349626693763
2	16.812869333521732	19.92690287190719	37.056938549966944	26.203289244604132
3	19.739029372758086	23.376080712003247	28.899697410732127	27.98519250450654
4	14.09360858871155	15.658417022205425	34.015206481954934	36.232767907128085
5	14.54623869306407	36.923574652274816	33.62723880107363	14.90294785358748
6	35.22065916530557	34.66874315403864	15.787200080341085	14.323397600314703
7	29.87197813774683	31.126509117473024	20.006131726131375	18.995381018648775
8	27.5440441756508	35.12720788669214	18.52918925002637	18.799558687630686
9	26.67754634759218	15.15163828712161	18.41493682936888	39.75587853591733
10-11	26.42105350825345	25.221636597749512	26.635093588589783	21.722216305407258
12-13	26.683133140831888	22.222042757226724	27.119611638871394	23.975212463069994
14-15	23.73650187853997	23.735290365073176	24.12871674348209	28.399491012904765
16-17	24.214041352202013	26.89637363638727	24.877960494513996	24.01162451689672
18-19	24.24767169457873	25.958013322416644	26.399195157109578	23.395119825895044
20-21	24.950130025358984	25.101014460273618	26.264680852352573	23.68417466201483
22-23	24.58330383590267	24.79861299610735	26.269711381995325	24.348371785994658
24-25	24.2987181048369	25.1547870900658	26.28540638354389	24.261088421553403
26-27	24.120648544097044	25.383042697872376	26.29073590635057	24.205572851680014
28-29	24.165309412857493	25.414618062027056	26.39688831240742	24.023184212708035
30-31	24.372886955536327	25.32454331234007	26.178815537898732	24.12375419422487
32-33	24.195406077402797	25.325809509159058	26.076055050143616	24.40272936329453
34-35	24.23933925281919	25.379723041358282	26.269829160755826	24.1111085450667
36-37	24.44162984920321	25.1784502742127	26.19753511457164	24.182384762012454
38-39	24.369997652635483	25.312060657918316	26.136755437430114	24.181186252016083
40-41	24.35643389417237	25.288769105242665	26.22157127901716	24.133225721567808
42-43	24.4276696413799	25.183065828734208	26.061900436773367	24.327364093112525
44-45	24.3048329755366	25.25721044240638	26.08083913609346	24.357117445963564
46-47	24.442805089377106	25.33113640121315	25.95204949534088	24.274009014068866
48-49	24.41530136021626	25.517381943375224	25.719243690479498	24.34807300592902
50-51	24.3623646825534	25.68623135753183	25.70300210208475	24.24840185783002
52-53	24.532918688401452	25.46961794512997	25.68058761052655	24.316875755942032
54-55	24.366245406699765	25.465330955508676	25.67419620637603	24.49422743141553
56-57	24.31227960187252	25.652878186276734	25.676956034958813	24.35788617689193
58-59	24.289992229966735	25.466186221082115	25.754435904040278	24.489385644910868
60-61	24.299526523840857	25.560997873124848	25.826557502810527	24.31291810022377
62-63	24.168983854348706	25.606580598433364	25.802545946458356	24.421889600759574
64-65	24.046223104963897	25.56678574507393	25.822680416535032	24.564310733427146
66-67	24.133447479032164	25.65440988730952	25.712757473078767	24.49938516057955
68-69	24.25788824422086	25.451380146959067	25.779725453449814	24.511006155370254
70-71	24.03546955447872	25.440709593022703	25.855707419086343	24.668113433412234
72-73	24.192448302732274	25.48273122822677	25.70385551395872	24.620964955082236
74-75	24.061873417632064	25.641506141067183	25.64786621434431	24.648754226956438
76-77	24.239884038205204	25.356875653890835	25.69307854614679	24.710161761757163
78-79	24.138909312472755	25.47810747457737	25.693709847465485	24.68927336548439
80-81	24.150663438994872	25.570583278891718	25.636089890062213	24.6426633920512
82-83	24.114544293641547	25.429515228074994	25.764035231311023	24.69190524697244
84-85	23.997117857422275	25.58822712898724	25.571469312248098	24.843185701342392
86-87	24.05668306822777	25.494116589908977	25.70905579206314	24.740144549800117
88-89	23.926563248154704	25.413792259185207	25.633261489447683	25.026383003212405
90-91	23.848712150335242	25.485358902447118	25.681307217324427	24.98462172989321
92-93	23.873781558459324	25.467658691749467	25.82043602293816	24.838123726853052
94-95	23.84967619075501	25.34653917630641	25.82617385189082	24.97761078104776
96-97	23.86706826650448	25.10909189561601	25.889662673801922	25.13417716407759
98-99	23.746566597100532	25.286717377845562	25.79014011741525	25.176575907638654
100-101	23.698992841924184	25.252849525949728	25.90702527208108	25.141132360045006
102-103	23.51647885692277	25.130543904118973	25.98531022996272	25.367667008995536
104-105	23.540674083005705	25.28375983276957	25.737762945940982	25.437803138283744
106-107	23.493664484483315	25.260752257060133	25.804028974253534	25.441554284203022
108-109	23.396528589032982	25.260676328795018	25.852452036993995	25.490343045178008
110-111	23.368042511624232	25.35749082383749	25.833653117775274	25.440813546763003
112-113	23.342663745066773	25.39909338882455	25.82500582309549	25.43323704301318
114-115	23.323188908504218	25.54422868702357	25.66244641938059	25.47013598509162
116-117	23.13153568834719	25.42943311396923	25.803890613036778	25.6351405846468
118-119	23.234994092075308	25.41675630015664	25.664706190356927	25.683543417411126
120-121	23.107322663932024	25.50607067805472	25.755402999356043	25.631203658657213
122-123	23.04003235886241	25.52245974792436	25.64928105365139	25.78822683956184
124-125	23.026260723142293	25.615721817345616	25.61000289228638	25.748014567225713
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	43.0
1	59.0
2	153.5
3	289.0
4	289.0
5	229.0
6	292.5
7	591.0
8	929.5
9	980.5
10	800.5
11	671.0
12	721.0
13	766.0
14	867.5
15	1031.0
16	1141.0
17	1290.0
18	1512.0
19	1773.0
20	2008.5
21	2342.5
22	2899.5
23	3616.0
24	4492.0
25	5711.0
26	7382.5
27	9653.0
28	12452.5
29	16234.0
30	21078.5
31	26214.5
32	32193.5
33	36759.5
34	40751.5
35	46550.5
36	53724.0
37	61962.0
38	70842.5
39	78547.5
40	86323.0
41	95832.5
42	104393.5
43	114606.0
44	125830.5
45	136653.0
46	148100.0
47	157083.5
48	165009.5
49	172039.5
50	177463.5
51	182204.5
52	183888.0
53	184220.0
54	182842.5
55	174486.0
56	162440.0
57	149132.0
58	135878.0
59	121328.5
60	104013.0
61	87566.0
62	70462.5
63	52532.0
64	37636.5
65	27381.0
66	20162.5
67	15107.5
68	11067.0
69	7981.5
70	5838.0
71	4218.0
72	2991.5
73	2150.0
74	1530.0
75	1094.5
76	756.0
77	450.0
78	254.0
79	148.5
80	90.5
81	59.0
82	35.5
83	21.5
84	12.0
85	10.5
86	8.5
87	6.0
88	6.5
89	7.5
90	5.5
91	3.5
92	4.0
93	5.5
94	6.0
95	7.5
96	9.0
97	5.0
98	1.0
99	1.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05041351445528046
2	7.569596765057126E-5
3	0.002397038975601423
4	0.12391429904398515
5	0.01541674541149968
6	0.0030278387060228506
7	0.0057528935414434155
8	0.012540298640777972
9	0.027679492170892227
10-11	0.022229382500051093
12-13	0.020904703066166097
14-15	0.03119935466664379
16-17	0.04027025479010391
18-19	0.043007925620132906
20-21	0.023188198090291663
22-23	0.02557262107128466
24-25	0.023352206020201237
26-27	0.021977062607882524
28-29	0.02198967860249095
30-31	0.027212700370380368
32-33	0.026581900639958946
34-35	0.03087133880682465
36-37	0.03368470560450421
38-39	0.033634241626070496
40-41	0.036599000359051205
42-43	0.033520697674594646
44-45	0.03137597859116179
46-47	0.04132999833721191
48-49	0.03413888141040764
50-51	0.024374101583483947
52-53	0.03227171420836021
54-55	0.033773017566763215
56-57	0.02712438840812137
58-59	0.030442394990138077
60-61	0.044849860832963474
62-63	0.052066209748984606
64-65	0.046111460293806326
66-67	0.04086320653670005
68-69	0.031174122677426933
70-71	0.038239079658146914
72-73	0.041632782207814197
74-75	0.025345533168332945
76-77	0.022292462473093237
78-79	0.03280158598191421
80-81	0.02592586892032066
82-83	0.0397782310003752
84-85	0.02253216637065338
86-87	0.03545094484968421
88-89	0.03512292898986506
90-91	0.036939632213478776
92-93	0.02755333222480794
94-95	0.023629757901586664
96-97	0.01862120804204053
98-99	0.02639266072083251
100-101	0.024853509378604234
102-103	0.02891585964251822
104-105	0.02587540494188694
106-107	0.015177041513939537
108-109	0.01872213599890796
110-111	0.023175582095683236
112-113	0.013978522026138827
114-115	0.015631217319842967
116-117	0.014054217993789397
118-119	0.008213012490086982
120-121	0.006030445422828845
122-123	0.004201126204606705
124-125	0.06783620300952029
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	3963223.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.5626854268151
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.75048367051127	36.05709336810199
2	13.330972159068027	13.214375591070377
3	5.231015937342974	7.777895920955584
4	2.5905546172073755	5.135793742945303
5	1.529416617854141	3.790099735862414
6	0.9678373076719765	2.87811696146891
7	0.6526190949925521	2.2641888436054036
8	0.496233044394008	1.9675713822152794
9	0.35681050896500166	1.5916038311532752
>10	1.9373496802055197	17.22015393284104
>50	0.11420691780284944	3.8292492516720014
>100	0.040554334402297804	3.388913567644547
>500	0.0014851889194030144	0.49592800051177466
>1k	4.6092066259500547E-4	0.38901586995198245
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.2615994608428544E-4
2	0.0	0.0	0.0	0.0	1.2615994608428544E-4
3	0.0	0.0	0.0	0.0	1.2615994608428544E-4
4	0.0	0.0	0.0	2.523198921685709E-5	1.2615994608428544E-4
5	0.0	0.0	0.0	2.523198921685709E-5	1.5139193530114254E-4
6	0.0	0.0	0.0	2.523198921685709E-5	2.2708790295171378E-4
7	0.0	0.0	0.0	2.523198921685709E-5	2.523198921685709E-4
8	0.0	0.0	0.0	2.523198921685709E-5	2.523198921685709E-4
9	0.0	0.0	0.0	5.046397843371418E-5	2.77551881385428E-4
10-11	0.0	0.0	0.0	5.046397843371418E-5	3.280158598191421E-4
12-13	0.0	0.0	0.0	7.569596765057127E-5	5.929517465961417E-4
14-15	0.0	0.0	0.0	1.0092795686742836E-4	7.443436818972841E-4
16-17	0.0	0.0	0.0	1.0092795686742836E-4	8.831196225899981E-4
18-19	0.0	0.0	0.0	1.0092795686742836E-4	0.0010092795686742835
20-21	0.0	0.0	0.0	1.38775940692714E-4	0.0011480555093669976
22-23	0.0	0.0	0.0	2.6493588677699943E-4	0.0012868314500597115
24-25	0.0	0.0	0.0	5.55103762770856E-4	0.00129944744466814
26-27	0.0	0.0	0.0	9.209676064152836E-4	0.0014256073907524254
28-29	0.0	0.0	0.0	0.0033306225766251358	0.001463455374577711
30-31	2.523198921685709E-5	0.0	0.0	0.012300594743217829	0.001463455374577711
32-33	2.523198921685709E-5	0.0	0.0	0.03102273074212579	0.0014886873637945683
34-35	2.523198921685709E-5	0.0	0.0	0.05379460101033931	0.0015643833314451395
36-37	2.523198921685709E-5	0.0	0.0	0.09122625701354681	0.001576999326053568
38-39	5.046397843371418E-5	0.0	0.0	0.15192180707469652	0.0015896153206619965
40-41	5.046397843371418E-5	0.0	0.0	0.21959400215430724	0.0015896153206619965
42-43	5.046397843371418E-5	0.0	0.0	0.28460422237153954	0.0016148473098788535
44-45	5.046397843371418E-5	0.0	0.0	0.3605272779250625	0.0017031592721378536
46-47	5.046397843371418E-5	0.0	0.0	0.44968451182282704	0.001741007255963139
48-49	5.046397843371418E-5	0.0	0.0	0.5446072552566434	0.0017914712343968533
50-51	5.046397843371418E-5	0.0	0.0	0.6480710270403659	0.0018419352128305675
52-53	5.046397843371418E-5	0.0	0.0	0.7495414716759566	0.0019176311804811387
54-55	5.046397843371418E-5	0.0	0.0	0.8591996968124176	0.001968095158914853
56-57	5.046397843371418E-5	0.0	0.0	0.98915705727384	0.0020311751319569958
58-59	5.046397843371418E-5	0.0	0.0	1.127112958317006	0.0021068710996075667
60-61	5.046397843371418E-5	0.0	0.0	1.279097845364745	0.0021447190834328524
62-63	5.046397843371418E-5	0.0	0.0	1.4342998110376328	0.0023465749971677092
64-65	5.046397843371418E-5	0.0	0.0	1.5929585592332303	0.0023591909917761375
66-67	5.046397843371418E-5	0.0	0.0	1.7758652490662272	0.002371806986384566
68-69	5.046397843371418E-5	0.0	0.0	1.9811148653507513	0.002371806986384566
70-71	5.046397843371418E-5	0.0	0.0	2.198753388340752	0.002409654970209852
72-73	5.046397843371418E-5	0.0	0.0	2.4208453574275284	0.002573662900119423
74-75	5.046397843371418E-5	0.0	0.0	2.6515666668264695	0.002788134808462708
76-77	5.046397843371418E-5	0.0	0.0	2.9086427889624176	0.002901678759938565
78-79	5.046397843371418E-5	0.0	0.0	3.1958459062232936	0.002964758732980708
80-81	5.046397843371418E-5	0.0	0.0	3.503587862706691	0.003027838706022851
82-83	5.046397843371418E-5	0.0	0.0	3.826267156806468	0.003153998652107136
84-85	7.569596765057127E-5	0.0	0.0	4.148858138943986	0.003153998652107136
86-87	7.569596765057127E-5	0.0	0.0	4.4972488300557405	0.0033306225766251358
88-89	7.569596765057127E-5	0.0	0.0	4.884004760771726	0.003507246501143135
90-91	7.569596765057127E-5	0.0	0.0	5.302527765911734	0.003507246501143135
92-93	7.569596765057127E-5	0.0	0.0	5.733199973859659	0.003658638436444278
94-95	7.569596765057127E-5	0.0	0.0	6.1675434362386365	0.003683870425661135
96-97	7.569596765057127E-5	0.0	0.0	6.622198649937185	0.0041128142423477055
98-99	7.569596765057127E-5	0.0	0.0	7.11203987259864	0.004466062091383704
100-101	7.569596765057127E-5	0.0	0.0	7.6514241060873935	0.004466062091383704
102-103	7.569596765057127E-5	0.0	0.0	8.209530475575056	0.00450391007520899
104-105	7.569596765057127E-5	0.0	0.0	8.761177455823201	0.004541758059034276
106-107	7.569596765057127E-5	0.0	0.0	9.325932454469505	0.004554374053642705
108-109	7.569596765057127E-5	0.0	0.0	9.925633758181156	0.004604838032076419
110-111	7.569596765057127E-5	0.0	0.0	10.564760045044148	0.0046931499943354184
112-113	7.569596765057127E-5	0.0	0.0	11.226620354191525	0.0046931499943354184
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5605	0.0	44.01307	1
GTATCAA	8540	0.0	43.39997	1
TCAACGC	10575	0.0	34.136375	4
TATCAAC	11180	0.0	32.65589	2
CAACGCA	11025	0.0	32.597507	5
ATCAACG	11400	0.0	31.874996	3
AACGCAG	11550	0.0	31.162596	6
GTACATG	14955	0.0	26.576412	1
ACGCAGA	13735	0.0	26.075884	7
TACATGG	15690	0.0	24.901402	2
ACATGGG	15035	0.0	24.881777	3
CGCAGAG	14480	0.0	24.653032	8
CATGGGG	11170	0.0	24.371834	4
GCAGAGT	16115	0.0	21.118296	9
GGACCGT	765	0.0	20.219584	6
AGAGTAC	14275	0.0	20.193846	10-11
GTATATA	1510	0.0	18.941797	1
CAGAGTA	15430	0.0	18.701532	10-11
TATACCG	385	5.0276867E-9	18.545841	5
GAGTACT	7720	0.0	18.189728	12-13
>>END_MODULE