FastQCFastQC Report
Mon 15 Aug 2016
SRR2921006_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921006_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3113519
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA55050.039.564261
TCAACGC59450.035.8300744
GGTATCA36300.035.4100381
CAACGCA62750.034.0389635
AACGCAG64350.033.101226
ATCAACG65700.032.5137063
TATCAAC69600.031.1192822
GTACATG104350.030.7950271
TACATGG111100.028.1178872
ACATGGG110100.027.8328293
ACGCAGA75250.027.5948777
CATGGGG74350.026.9690364
CGCAGAG77400.026.5204358
GCAGAGT90550.021.8144979
TACCGTA3151.016997E-820.7774227
GAGTACA76900.019.1139261
AGAGTAC78500.018.94856610-11
ATGGGGA42850.018.3281525
CAGAGTA85700.018.08560210-11
GTATTAG9950.017.9422741
Produced by FastQC (version 0.11.5)