##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921006_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3113519
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.719396284397174	31.0	30.0	34.0	20.0	34.0
2	28.749397064864546	31.0	29.0	34.0	18.0	34.0
3	28.845016201924576	31.0	30.0	34.0	20.0	34.0
4	32.3093891509896	35.0	33.0	37.0	22.0	37.0
5	32.40243595751303	35.0	33.0	37.0	24.0	37.0
6	32.36451327260248	35.0	33.0	37.0	23.0	37.0
7	32.26030963678076	35.0	33.0	37.0	22.0	37.0
8	32.250321902644565	35.0	33.0	37.0	22.0	37.0
9	33.434349364818395	37.0	33.0	39.0	19.0	39.0
10-11	33.384366853068826	37.0	33.0	39.0	18.5	39.0
12-13	33.31225744888661	37.0	33.0	39.0	18.0	39.0
14-15	34.236685563826654	38.0	33.0	41.0	18.0	41.0
16-17	34.114539689656624	38.0	33.0	41.0	17.0	41.0
18-19	34.21348352137886	38.5	33.0	41.0	18.0	41.0
20-21	34.235857722403495	39.0	33.0	41.0	18.0	41.0
22-23	34.19060298010065	39.0	33.0	41.0	18.0	41.0
24-25	34.11480723258795	38.5	33.0	41.0	18.0	41.0
26-27	33.97398715087334	38.0	33.0	41.0	18.0	41.0
28-29	33.903182058628836	38.0	33.0	41.0	18.0	41.0
30-31	33.77635530729056	38.0	33.0	40.0	17.0	41.0
32-33	33.68740210032443	38.0	32.5	40.0	16.0	41.0
34-35	33.5248766749135	38.0	32.0	40.0	16.0	41.0
36-37	33.37811299690158	38.0	32.0	40.0	15.0	41.0
38-39	33.24461164360969	38.0	32.0	40.0	13.0	41.0
40-41	33.16499160596097	38.0	32.0	40.0	10.5	41.0
42-43	32.9502681692323	38.0	31.5	40.0	9.0	41.0
44-45	32.78317668849941	38.0	31.0	40.0	8.5	41.0
46-47	32.595706016247206	38.0	31.0	40.0	8.0	41.0
48-49	32.42853086812703	37.5	31.0	40.0	6.5	41.0
50-51	32.19844603485638	37.0	31.0	40.0	2.0	41.0
52-53	32.01285747734316	37.0	30.0	40.0	2.0	41.0
54-55	31.79868357957668	37.0	30.0	40.0	2.0	41.0
56-57	31.59125879752139	36.0	30.0	40.0	2.0	41.0
58-59	31.331174950273308	36.0	30.0	39.5	2.0	41.0
60-61	31.13524423650538	36.0	29.5	39.0	2.0	41.0
62-63	31.0174705855336	35.0	30.0	39.0	2.0	41.0
64-65	30.783148264070334	35.0	29.0	39.0	2.0	41.0
66-67	30.514401068373118	35.0	29.0	39.0	2.0	40.0
68-69	30.200932610335766	35.0	29.0	38.0	2.0	40.0
70-71	29.868166534394042	35.0	29.0	37.0	2.0	40.0
72-73	29.58666496013032	34.0	29.0	37.0	2.0	39.0
74-75	29.282612535847704	34.0	29.0	36.0	2.0	39.0
76-77	29.00207466214274	34.0	28.5	36.0	2.0	39.0
78-79	28.681808590215766	34.0	28.0	35.5	2.0	37.5
80-81	28.366638038823595	34.0	27.5	35.0	2.0	37.0
82-83	28.096935332657356	34.0	27.0	35.0	2.0	36.5
84-85	27.85970793818827	34.0	27.0	35.0	2.0	36.0
86-87	27.7925601224852	34.0	27.0	35.0	2.0	36.0
88-89	27.88287449024721	34.0	27.0	35.0	2.0	36.0
90-91	27.794338817267537	34.0	27.0	35.0	2.0	35.0
92-93	27.642626237386057	34.0	27.0	35.0	2.0	35.0
94-95	27.559397742554324	34.0	27.0	35.0	2.0	35.0
96-97	27.462712287928866	34.0	27.0	35.0	2.0	35.0
98-99	27.398268486558138	34.0	27.0	35.0	2.0	35.0
100-101	27.226871106294837	34.0	26.5	35.0	2.0	35.0
102-103	25.855705071978043	31.5	24.0	34.0	2.0	35.0
104-105	26.897763431024508	33.0	25.0	35.0	2.0	35.0
106-107	27.09114831160497	34.0	26.0	35.0	2.0	35.0
108-109	27.07951019409228	34.0	26.0	35.0	2.0	35.0
110-111	26.96687879534379	34.0	25.0	35.0	2.0	35.0
112-113	26.865065862774564	34.0	25.0	35.0	2.0	35.0
114-115	26.7285903827791	34.0	25.0	35.0	2.0	35.0
116-117	26.588620143316938	34.0	25.0	35.0	2.0	35.0
118-119	26.386773454730807	34.0	25.0	35.0	2.0	35.0
120-121	26.164087323700286	34.0	24.0	35.0	2.0	35.0
122-123	25.923955177405375	34.0	23.5	35.0	2.0	35.0
124-125	24.739852719703975	32.0	11.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	158114.0
3	23233.0
4	15173.0
5	13652.0
6	15734.0
7	17395.0
8	18293.0
9	19352.0
10	20727.0
11	21489.0
12	21758.0
13	22115.0
14	22205.0
15	20356.0
16	17213.0
17	15697.0
18	14467.0
19	14377.0
20	14454.0
21	15402.0
22	19003.0
23	24880.0
24	33851.0
25	43060.0
26	49888.0
27	57343.0
28	67218.0
29	79503.0
30	92445.0
31	111257.0
32	135478.0
33	168660.0
34	217988.0
35	297810.0
36	463744.0
37	578803.0
38	171159.0
39	223.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.200194063706874	18.36070788271355	11.87393570193871	25.56516235164087
2	16.312986952077523	20.254989903584534	37.849543814202825	25.582479330135115
3	19.621888316054054	23.502530210898026	29.135783273108572	27.739798199939347
4	13.100230045016351	15.512731181427808	35.76555903894446	35.621479734611384
5	13.866241708808571	37.55506179866776	34.3202403526011	14.258456139922579
6	33.849856972073425	35.51804503018908	16.56168958603806	14.070408411699448
7	29.000096037443683	32.09047819259458	20.50739889810684	18.402026871854893
8	26.76348900903887	35.22959813781445	19.406545593770787	18.60036725937589
9	26.307666760775184	15.96889858024614	18.841488816021702	38.88194584295697
10-11	25.328967865730235	26.074108025569686	27.427735954626716	21.169188154073364
12-13	25.648657972282557	23.462534939102696	27.30755468359033	23.581252405024422
14-15	22.830957684778895	25.05975322076404	24.409843097509324	27.69944599694774
16-17	23.328226999375453	27.978159943612457	25.367682542874014	23.32593051413808
18-19	23.323785722842867	27.23676971298393	26.708139568122114	22.73130499605109
20-21	24.04201010215861	26.36996795137397	26.497786659831686	23.090235286635743
22-23	23.713258526713314	26.161327729983142	26.35445477742132	23.770958965882226
24-25	23.402951105789445	26.414487725459352	26.586626462954438	23.59593470579677
26-27	23.255754945158536	26.54860349747525	26.466396304193275	23.72924525317294
28-29	23.326137065692564	26.836537768199886	26.43901665034217	23.398308515765383
30-31	23.535238412139197	26.670278910975792	26.31525867560927	23.479224001275742
32-33	23.239797155565775	26.65108194297192	26.24026061829075	23.86886028317155
34-35	23.540295742260913	26.72198062582087	26.40622435413782	23.331499277780402
36-37	23.658758373826636	26.380765959415047	26.38266107169563	23.577814595062687
38-39	23.419474723752447	26.667012476286477	26.361741807724602	23.551770992236467
40-41	23.49084513443313	26.63203982937426	26.505011593133215	23.37210344305939
42-43	23.4315738117251	26.53637955419928	26.31686292118519	23.715183712890422
44-45	23.415694602627674	26.59588408846955	26.41086591907828	23.577555389824497
46-47	23.461871134529808	26.68330711178251	26.309266527407342	23.54555522628034
48-49	23.34829919321826	26.728922973598646	26.269412136719772	23.65336569646333
50-51	23.320914219783297	26.887905481541253	26.26032213675137	23.530858161924083
52-53	23.57297966926217	26.63852115795177	26.176832677953776	23.61166649483228
54-55	23.29009922550533	26.65841553948368	26.287917767512848	23.763567467498138
56-57	23.270383994393466	26.814118732272814	26.235944717037913	23.679552556295807
58-59	23.31728340913431	26.522910609668532	26.308749234937196	23.851056746259967
60-61	23.17308867401456	26.678163911701287	26.522406700241962	23.62634071404219
62-63	23.210662044644252	26.604231675233116	26.56660172301175	23.61850455711088
64-65	23.20920053134842	26.56114112388445	26.398367088133913	23.83129125663322
66-67	23.130224199979764	26.762758490002458	26.265940584837395	23.841076725180383
68-69	23.41923691945782	26.449724392371216	26.380989104011427	23.750049584159534
70-71	23.274421670207317	26.408766471743405	26.437047795919653	23.87976406212962
72-73	23.361042380097796	26.330222155266814	26.4353557115017	23.87337975313369
74-75	23.257650688791237	26.614910767324407	26.326309157829638	23.80112938605472
76-77	23.33542638920561	26.33770359116998	26.451146148830723	23.87572387079368
78-79	23.35205878531819	26.465485690775186	26.408058330524327	23.7743971933823
80-81	23.487438352879845	26.358476969845206	26.382518322899625	23.771566354375327
82-83	23.40773711065046	26.417521716982574	26.482593974167663	23.692147198199304
84-85	23.368033568916793	26.444964798984287	26.314804789695636	23.87219684240329
86-87	23.267185998783848	26.39628460496463	26.494354021922195	23.842175374329326
88-89	23.326435457756965	26.26916681735361	26.33094578835074	24.07345193653869
90-91	23.19556909589238	26.36098237495445	26.446738079435157	23.99671044971801
92-93	23.333043175487465	26.282438790613533	26.577837679491	23.806680354407998
94-95	23.320071454508458	26.103588480361495	26.652297177649704	23.924042887480347
96-97	23.328711866676922	25.85846299322968	26.671360402290826	24.141464737802565
98-99	23.195084337395834	26.143123289994318	26.493358790406802	24.168433582203043
100-101	23.179268413144438	26.09813116010058	26.632064766533542	24.09053566022144
102-103	23.03741826273327	25.980899397013253	26.771615858334652	24.21006648191883
104-105	22.95074516313673	26.20554605146087	26.536210386608584	24.30749839879381
106-107	22.957278877474316	26.24445065499939	26.42715340284872	24.371117064677573
108-109	22.88410804817088	26.25429345743418	26.572393964088658	24.28920453030628
110-111	22.970347251635967	26.366038525620112	26.447923176841776	24.215691045902147
112-113	22.971892000401485	26.475318679112718	26.481774565893808	24.071014754591992
114-115	22.945668229906236	26.589254747659595	26.387284773487664	24.0777922489465
116-117	22.886392635544066	26.494584179492552	26.42353490993975	24.195488275023628
118-119	23.041728650789203	26.39745522890108	26.293564405966602	24.267251714343114
120-121	22.972724256322675	26.588028249315272	26.30745590099699	24.131791593365065
122-123	22.991608736627267	26.560577765015076	26.26967485299871	24.17813864535895
124-125	23.09990195136389	26.61461294248424	26.162772947831765	24.122712158320105
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1014.0
1	824.0
2	553.0
3	416.0
4	330.0
5	290.0
6	278.5
7	298.0
8	345.5
9	393.0
10	411.0
11	437.0
12	500.0
13	587.0
14	658.0
15	706.5
16	838.5
17	1018.0
18	1186.0
19	1487.0
20	1862.5
21	2301.5
22	3113.0
23	4167.0
24	5394.5
25	7238.5
26	9934.5
27	13312.5
28	16724.0
29	20795.0
30	25615.5
31	31421.0
32	39174.5
33	44374.5
34	48118.5
35	53225.5
36	58985.5
37	65653.0
38	71951.0
39	76582.0
40	81063.5
41	86287.5
42	91367.0
43	98281.5
44	104410.0
45	110793.0
46	117989.5
47	121260.5
48	123809.0
49	127097.5
50	128728.0
51	129163.0
52	127301.0
53	125328.0
54	123115.5
55	117067.5
56	108306.0
57	98855.0
58	90469.0
59	81066.0
60	68875.5
61	57222.0
62	46978.5
63	36143.0
64	26502.0
65	19497.0
66	14326.5
67	10631.5
68	7746.5
69	5695.5
70	4350.0
71	3201.0
72	2225.0
73	1711.5
74	1344.5
75	1007.0
76	720.5
77	473.5
78	318.0
79	206.5
80	138.5
81	90.5
82	74.0
83	52.0
84	33.0
85	29.5
86	23.5
87	16.5
88	14.0
89	10.5
90	8.0
91	8.0
92	9.5
93	9.5
94	9.0
95	6.5
96	3.5
97	4.0
98	6.0
99	8.5
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.036582400813998564
2	0.017118893445005477
3	0.02360672923466984
4	0.020876699323177407
5	0.013682267556420886
6	0.00452863785318156
7	0.004817699843810171
8	0.006038183804242081
9	0.0029548559042035715
10-11	0.004657109849016499
12-13	0.007868909744889945
14-15	0.002842442907848001
16-17	0.002103728931797108
18-19	0.0
20-21	0.0040147498698418094
22-23	7.868909744889945E-4
24-25	0.001348955956266848
26-27	0.0014292509536636842
28-29	0.0031957408963940804
30-31	0.0011080709640763394
32-33	0.0
34-35	0.006423599791746895
36-37	0.007933145742807415
38-39	0.00168619494533356
40-41	0.00122048396043191
42-43	0.0028103249088892667
44-45	0.0013168379573081136
46-47	0.019961336352853475
48-49	0.0031957408963940804
50-51	0.009860225680331484
52-53	0.0017986079416891308
54-55	1.6058999479367237E-4
56-57	3.2117998958734474E-4
58-59	0.006825074778731076
60-61	6.263009796953223E-4
62-63	0.00987628467981085
64-65	0.00987628467981085
66-67	1.284719958349379E-4
68-69	0.004030808869321177
70-71	0.005042525836521312
72-73	0.010791647650134784
74-75	0.001846784940127232
76-77	0.001573781948977989
78-79	6.584189786540568E-4
80-81	0.004287752860991052
82-83	0.0018307259406478652
84-85	0.0013971329547049496
86-87	0.013425323564751011
88-89	0.0
90-91	7.547729755302601E-4
92-93	0.008703977717817044
94-95	0.006728720781854873
96-97	0.004801640844330804
98-99	0.0013650149557462151
100-101	0.015785996488217994
102-103	0.008559446722502738
104-105	0.007740437749055008
106-107	0.01731160143875788
108-109	0.011305535633474533
110-111	0.019383212371596258
112-113	0.0026176169151368596
114-115	0.01389103454965266
116-117	0.005990006805803979
118-119	0.004962230839124476
120-121	0.007708319750096273
122-123	0.009025157707404386
124-125	0.057748162127804585
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	3113519.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.500360441191475
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.25453859672028	35.98379882205164
2	13.917292340741705	14.05656559145779
3	5.709989188603162	8.650695364192982
4	2.9498816827172374	5.958803529443556
5	1.6773879284106743	4.235434749222127
6	1.0505982138785879	3.1833353087844394
7	0.711908109533023	2.5166131292697416
8	0.4916508706210607	1.986283694207133
9	0.3672173852794486	1.6690149285195628
>10	1.752999017967491	15.206247168534745
>50	0.08281447024786326	2.830430874824696
>100	0.03135319864659067	2.6847314498684103
>500	0.0017927542090181655	0.6360065044105949
>1k	5.76242423968165E-4	0.4020388852126025
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.2117998958734476E-5
2	0.0	0.0	0.0	0.0	3.2117998958734476E-5
3	0.0	0.0	0.0	0.0	3.2117998958734476E-5
4	0.0	0.0	0.0	0.0	3.2117998958734476E-5
5	0.0	0.0	0.0	0.0	6.423599791746895E-5
6	0.0	0.0	0.0	0.0	1.6058999479367237E-4
7	0.0	0.0	0.0	0.0	1.6058999479367237E-4
8	0.0	0.0	0.0	3.2117998958734476E-5	1.6058999479367237E-4
9	0.0	0.0	0.0	3.2117998958734476E-5	1.6058999479367237E-4
10-11	0.0	0.0	0.0	3.2117998958734476E-5	1.9270799375240683E-4
12-13	0.0	0.0	0.0	6.423599791746895E-5	5.941829807365878E-4
14-15	0.0	0.0	0.0	6.423599791746895E-5	7.065959770921585E-4
16-17	0.0	0.0	0.0	6.423599791746895E-5	7.708319750096273E-4
18-19	0.0	0.0	0.0	6.423599791746895E-5	7.708319750096273E-4
20-21	0.0	0.0	0.0	1.766489942730396E-4	8.029499739683619E-4
22-23	0.0	0.0	0.0	4.335929859429154E-4	8.350679729270963E-4
24-25	0.0	0.0	0.0	8.350679729270963E-4	8.671859718858307E-4
26-27	0.0	0.0	0.0	0.0013168379573081134	9.795989682414015E-4
28-29	0.0	0.0	0.0	0.004865876842248273	9.956579677207686E-4
30-31	0.0	0.0	0.0	0.019318976373678787	0.001011716967200136
32-33	0.0	0.0	0.0	0.0478879364474731	0.0011080709640763392
34-35	0.0	3.2117998958734476E-5	0.0	0.08736095716775777	0.0013168379573081134
36-37	0.0	3.2117998958734476E-5	0.0	0.14944504915499152	0.0013168379573081134
38-39	0.0	3.2117998958734476E-5	0.0	0.24735676898069356	0.0013489559562668478
40-41	0.0	3.2117998958734476E-5	0.0	0.3519169145908536	0.0013489559562668478
42-43	0.0	3.2117998958734476E-5	0.0	0.4541324462770261	0.0013489559562668478
44-45	0.0	3.2117998958734476E-5	0.0	0.5759078393290678	0.0013971329547049496
46-47	0.0	3.2117998958734476E-5	0.0	0.7190256426891887	0.0014613689526224187
48-49	0.0	3.2117998958734476E-5	0.0	0.8689203438296025	0.0015737819489779893
50-51	0.0	3.2117998958734476E-5	0.0	1.0298796956113003	0.001702253944812927
52-53	0.0	3.2117998958734476E-5	0.0	1.1868403565226355	0.0018949619385653339
54-55	0.0	3.2117998958734476E-5	0.0	1.36540037173372	0.001959197936482803
56-57	0.0	3.2117998958734476E-5	0.0	1.5715979250487953	0.001991315935441537
58-59	0.0	3.2117998958734476E-5	0.0	1.792858819875517	0.001991315935441537
60-61	0.0	3.2117998958734476E-5	0.0	2.021121438475243	0.001991315935441537
62-63	0.0	3.2117998958734476E-5	0.0	2.247858452124429	0.001991315935441537
64-65	0.0	3.2117998958734476E-5	0.0	2.4934166131634337	0.0020555519333590065
66-67	0.0	3.2117998958734476E-5	0.0	2.776841894974786	0.0020876699323177407
68-69	0.0	3.2117998958734476E-5	0.0	3.0987766575376607	0.0020876699323177407
70-71	0.0	3.2117998958734476E-5	0.0	3.43025046579128	0.0020876699323177407
72-73	3.2117998958734476E-5	3.2117998958734476E-5	0.0	3.7681799918356047	0.00215190593023521
74-75	3.2117998958734476E-5	3.2117998958734476E-5	0.0	4.117575643508197	0.00226431892659078
76-77	3.2117998958734476E-5	3.2117998958734476E-5	0.0	4.510956894754777	0.0023446139239876166
78-79	3.2117998958734476E-5	3.2117998958734476E-5	0.0	4.944148405710709	0.0023446139239876166
80-81	3.2117998958734476E-5	3.2117998958734476E-5	0.0	5.39460334110696	0.0023446139239876166
82-83	3.2117998958734476E-5	3.2117998958734476E-5	0.0	5.860153736013816	0.0024088499219050855
84-85	3.2117998958734476E-5	3.2117998958734476E-5	0.0	6.3468217152360396	0.002424908921384453
86-87	3.2117998958734476E-5	3.2117998958734476E-5	0.0	6.8757890990869175	0.002505203918781289
88-89	3.2117998958734476E-5	3.2117998958734476E-5	0.0	7.4415958277434635	0.002569439916698758
90-91	3.2117998958734476E-5	3.2117998958734476E-5	0.0	8.044466727198389	0.002569439916698758
92-93	3.2117998958734476E-5	3.2117998958734476E-5	0.0	8.65430723242736	0.0026979119125336956
94-95	3.2117998958734476E-5	3.2117998958734476E-5	0.0	9.27328530836009	0.0026979119125336956
96-97	3.2117998958734476E-5	3.2117998958734476E-5	0.0	9.937790647816827	0.002970914903682939
98-99	3.2117998958734476E-5	3.2117998958734476E-5	0.0	10.653090602626804	0.0032117998958734475
100-101	3.2117998958734476E-5	3.2117998958734476E-5	0.0	11.410127897083655	0.0032117998958734475
102-103	3.2117998958734476E-5	3.2117998958734476E-5	0.0	12.178165606183871	0.003292094893270284
104-105	3.2117998958734476E-5	3.2117998958734476E-5	0.0	12.94893334519558	0.00337238989066712
106-107	3.2117998958734476E-5	3.2117998958734476E-5	0.0	13.737109039642926	0.00337238989066712
108-109	3.2117998958734476E-5	3.2117998958734476E-5	0.0	14.566460008755367	0.00337238989066712
110-111	3.2117998958734476E-5	3.2117998958734476E-5	0.0	15.43713078352822	0.00337238989066712
112-113	3.2117998958734476E-5	3.2117998958734476E-5	0.0	16.32007063390331	0.00337238989066712
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	5505	0.0	39.56426	1
TCAACGC	5945	0.0	35.830074	4
GGTATCA	3630	0.0	35.410038	1
CAACGCA	6275	0.0	34.038963	5
AACGCAG	6435	0.0	33.10122	6
ATCAACG	6570	0.0	32.513706	3
TATCAAC	6960	0.0	31.119282	2
GTACATG	10435	0.0	30.795027	1
TACATGG	11110	0.0	28.117887	2
ACATGGG	11010	0.0	27.832829	3
ACGCAGA	7525	0.0	27.594877	7
CATGGGG	7435	0.0	26.969036	4
CGCAGAG	7740	0.0	26.520435	8
GCAGAGT	9055	0.0	21.814497	9
TACCGTA	315	1.016997E-8	20.777422	7
GAGTACA	7690	0.0	19.113926	1
AGAGTAC	7850	0.0	18.948566	10-11
ATGGGGA	4285	0.0	18.328152	5
CAGAGTA	8570	0.0	18.085602	10-11
GTATTAG	995	0.0	17.942274	1
>>END_MODULE