FastQCFastQC Report
Mon 15 Aug 2016
SRR2921006_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921006_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3113519
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45940.14755008721642615No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35790.11495031827331068No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49150.052.3814741
GTATCAA80050.045.7114071
TCAACGC97550.037.191174
CAACGCA101050.035.8551335
ATCAACG101750.035.8310743
TATCAAC103150.035.2252852
AACGCAG105750.034.4264766
ACGCAGA124350.029.0411137
CGCAGAG125150.028.7128588
GTACATG108750.027.7840921
ACATGGG110000.026.4283453
TACATGG113300.026.2340052
CATGGGG77000.025.4539384
GCAGAGT139000.024.4838379
AGAGTAC124450.022.4943510-11
GAGTACT78900.022.39781212-13
CAGAGTA133700.021.89487310-11
GTACTTT86300.020.61515414-15
AGTACTT89000.020.42430912-13
GTATAGG7650.019.4758361
Produced by FastQC (version 0.11.5)