FastQCFastQC Report
Mon 15 Aug 2016
SRR2921005_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921005_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1705662
Sequences flagged as poor quality0
Sequence length125
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA42700.035.2603531
GTACATG87050.029.3280031
TCAACGC49200.029.2645854
TACATGG85000.029.1891882
CAACGCA50100.028.8576355
CATGGGG48250.028.8543114
ATCAACG50000.028.7971953
CTATACG1055.908807E-428.3317154
ACATGGG86700.028.2737243
TATCAAC51700.028.195542
AACGCAG51850.027.997586
GGTATCA29500.024.8128761
ACGCAGA60300.023.9755387
ATGGGGA27100.023.9303675
CGCAGAG61850.023.2785058
TAGCCCT12650.020.6944714
GTGTAGC12000.020.3327731
GCAGAGT67450.020.1991429
ATAGTAC4900.019.4280323
CATGGGA39150.019.30034
Produced by FastQC (version 0.11.5)