FastQCFastQC Report
Mon 15 Aug 2016
SRR2921005_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921005_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1705662
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35120.20590245898659876No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26280.15407507466309267No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22500.131913591321141No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA64650.042.6788751
GGTATCA40800.037.830331
TATCAAC82950.032.815842
TCAACGC82800.032.735264
ATCAACG83450.032.6944353
CAACGCA83450.032.368035
AACGCAG85800.031.6876666
ACGCAGA98050.027.4261537
CGCAGAG98550.027.2878048
GTACATG90250.026.8749521
CATGGGG49750.026.7021684
TACATGG90700.025.874542
ACATGGG89850.025.4593833
GCAGAGT106750.024.3571119
GAGTACT53000.023.23842412-13
AGTACTT55350.023.16551212-13
AGAGTAC100000.022.22300710-11
ATGGGGA24100.021.9715025
GTACTTT57600.021.33093314-15
CTACACT8750.019.7435254
Produced by FastQC (version 0.11.5)