FastQCFastQC Report
Mon 15 Aug 2016
SRR2921004_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921004_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4105412
Sequences flagged as poor quality0
Sequence length125
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA84200.035.2690771
GTACATG170250.031.7747671
TCAACGC94100.030.6671264
TACATGG175300.029.8022772
CAACGCA96800.029.6255345
ACATGGG176750.028.951823
CATGGGG112100.028.6090954
AACGCAG101000.028.4517976
ATCAACG105350.027.7321663
TATCAAC106200.027.5662352
GGTATCA60050.025.5689141
ACGCAGA113550.025.0976077
CGCAGAG118000.023.999878
TACCGTA4300.023.521477
GCAGAGT136700.019.8898569
GTACCGT6950.018.83316
ATGGGGG64100.018.5637845
ATGGGGA66000.018.299815
AGAGTAC116900.017.81290810-11
TTATACT18750.016.8188234
Produced by FastQC (version 0.11.5)