FastQCFastQC Report
Mon 15 Aug 2016
SRR2921004_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921004_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4105412
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA86700.037.725851
GGTATCA61750.033.7688981
TCAACGC108100.029.5892314
CAACGCA110550.028.8472235
GTACATG167800.028.0137231
TATCAAC118600.027.2150822
ATCAACG117800.027.1524353
AACGCAG118100.027.0481596
TACATGG169950.026.5959592
CATGGGG110650.025.4083024
ACATGGG173200.025.2078553
ACGCAGA134200.023.6728027
CGCAGAG142000.022.247058
GCAGAGT159300.018.9358549
AGAGTAC137450.018.72235710-11
TACACCG5700.017.7448395
TATAGCG2702.4506186E-517.6288595
ATGGGGA66600.017.1524035
CAGAGTA146250.017.0262410-11
GTATAGG9450.017.022281
Produced by FastQC (version 0.11.5)