FastQCFastQC Report
Mon 15 Aug 2016
SRR2921003_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921003_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3107557
Sequences flagged as poor quality0
Sequence length125
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA32520.10464812069416586No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGGG77150.027.2237914
GTACATG143550.026.535451
GTATCAA96800.026.0699481
TACATGG143550.025.409032
ACATGGG143000.024.5497343
TATCAAC107700.022.7068542
AACGCAG106100.022.6541566
TCAACGC106850.022.5522754
CAACGCA107350.022.4457915
ATCAACG110200.022.0297433
ACGCAGA117250.020.347617
CGCAGAG118150.019.991198
GGTATCA73600.019.3272361
ACCGTCG2201.0228161E-418.9302888
ATGGGGA43300.017.3126895
GCAGAGT129550.017.0379929
ATGGGGG44700.016.7704585
TATACCG3558.110619E-716.759215
ATGGGGC33900.016.1461895
GTCTAGA18350.015.893141
Produced by FastQC (version 0.11.5)