##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921003_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3107557
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.82038012496633	31.0	28.0	31.0	15.0	34.0
2	27.93935782996096	31.0	28.0	34.0	15.0	34.0
3	27.969255913889914	31.0	28.0	34.0	16.0	34.0
4	31.38095680948089	35.0	32.0	37.0	16.0	37.0
5	31.422947028807517	35.0	32.0	37.0	16.0	37.0
6	31.374664406799297	35.0	32.0	37.0	16.0	37.0
7	31.246045366183147	35.0	32.0	37.0	13.0	37.0
8	31.245721639216914	35.0	32.0	37.0	13.0	37.0
9	32.340976529151355	37.0	32.0	39.0	10.0	39.0
10-11	32.27804075677454	37.0	32.0	39.0	10.0	39.0
12-13	32.188066059608886	37.0	32.0	39.0	10.0	39.0
14-15	33.013072326589665	38.0	32.0	40.0	10.0	41.0
16-17	32.866559004388336	38.0	32.0	40.0	10.0	41.0
18-19	32.96887651618297	38.0	32.0	40.0	10.0	41.0
20-21	32.98807503772256	38.0	32.0	40.0	10.0	41.0
22-23	32.928549822255874	38.0	32.0	40.0	10.0	41.0
24-25	32.84313626427448	38.0	31.5	40.0	8.5	41.0
26-27	32.67667769247676	38.0	31.0	40.0	8.0	41.0
28-29	32.580021701934996	38.0	31.0	40.0	7.5	41.0
30-31	32.43913096364765	38.0	31.0	40.0	2.0	41.0
32-33	32.32719319388188	38.0	30.5	40.0	2.0	41.0
34-35	32.1395963131167	38.0	30.0	40.0	2.0	41.0
36-37	31.974092832408225	38.0	30.0	40.0	2.0	41.0
38-39	31.836971292883767	38.0	30.0	40.0	2.0	41.0
40-41	31.751257177261753	37.5	30.0	40.0	2.0	41.0
42-43	31.53088471104472	37.0	30.0	40.0	2.0	41.0
44-45	31.35221574374983	37.0	30.0	40.0	2.0	41.0
46-47	31.141734648793246	37.0	29.5	40.0	2.0	41.0
48-49	30.969690016949002	37.0	29.0	40.0	2.0	41.0
50-51	30.73615045516462	36.5	28.5	40.0	2.0	41.0
52-53	30.5515828028255	36.0	28.0	40.0	2.0	41.0
54-55	30.327641134177107	36.0	28.0	40.0	2.0	41.0
56-57	30.121695434709643	36.0	28.0	39.5	2.0	41.0
58-59	29.860953636570464	35.0	28.0	39.0	2.0	41.0
60-61	29.663779779421585	35.0	27.5	39.0	2.0	41.0
62-63	29.537970663128625	35.0	27.5	39.0	2.0	41.0
64-65	29.31898240321899	35.0	27.0	39.0	2.0	40.0
66-67	29.059979752583782	35.0	26.5	38.0	2.0	40.0
68-69	28.758407327685383	34.0	26.0	38.0	2.0	40.0
70-71	28.429384078876105	34.0	26.0	37.0	2.0	40.0
72-73	28.150218483522586	34.0	26.0	36.5	2.0	39.0
74-75	27.855488571891037	34.0	26.0	36.0	2.0	39.0
76-77	27.573378058713	34.0	26.0	36.0	2.0	38.5
78-79	27.256920146597473	34.0	25.0	35.0	2.0	37.5
80-81	26.94195746047458	33.0	25.0	35.0	2.0	37.0
82-83	26.667785015689173	33.0	24.5	35.0	2.0	36.5
84-85	26.419682567367225	33.0	24.0	35.0	2.0	36.0
86-87	26.34185680262663	33.0	24.0	35.0	2.0	36.0
88-89	26.421083828872646	33.0	24.5	35.0	2.0	35.5
90-91	26.32245635397838	33.0	24.0	35.0	2.0	35.0
92-93	26.161599932036644	33.0	24.0	35.0	2.0	35.0
94-95	26.067594576704465	33.0	24.0	35.0	2.0	35.0
96-97	25.9614204984816	33.0	23.0	35.0	2.0	35.0
98-99	25.890137011163432	33.0	23.0	35.0	2.0	35.0
100-101	25.71020097137398	33.0	21.5	35.0	2.0	35.0
102-103	24.415347811802004	30.5	18.5	34.0	2.0	35.0
104-105	25.379716446070013	32.5	20.0	35.0	2.0	35.0
106-107	25.55319596712144	33.0	20.0	35.0	2.0	35.0
108-109	25.535544480760933	33.0	20.0	35.0	2.0	35.0
110-111	25.418583150687176	33.0	19.0	35.0	2.0	35.0
112-113	25.31231719965233	33.0	18.5	35.0	2.0	35.0
114-115	25.15978210536444	33.0	17.5	35.0	2.0	35.0
116-117	25.014560955760423	33.0	16.0	35.0	2.0	35.0
118-119	24.80395001604154	33.0	5.0	35.0	2.0	35.0
120-121	24.564449179854144	33.0	2.0	35.0	2.0	35.0
122-123	24.30859804019685	33.0	2.0	35.0	2.0	35.0
124-125	23.160444040125412	31.5	2.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	222294.0
3	30562.0
4	19215.0
5	17164.0
6	20578.0
7	21748.0
8	22511.0
9	23697.0
10	24700.0
11	25355.0
12	25237.0
13	25368.0
14	25465.0
15	22817.0
16	19695.0
17	17592.0
18	16427.0
19	15964.0
20	16195.0
21	17706.0
22	21123.0
23	27970.0
24	37066.0
25	46379.0
26	54676.0
27	61989.0
28	71271.0
29	82233.0
30	95947.0
31	112810.0
32	135723.0
33	166434.0
34	212641.0
35	283683.0
36	420954.0
37	502734.0
38	143484.0
39	150.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.72166583560092	18.398314733878156	12.668081570846235	25.211937859674684
2	15.766203603869206	20.13254612014056	39.241594930662885	24.859655345327347
3	19.436140079824902	24.360274237157203	28.65607699240376	27.547508690614137
4	12.895211340501506	16.083456017962703	35.91974204363088	35.10159059790491
5	14.077348998040623	37.497489630241844	33.972705200198774	14.45245617151876
6	33.41004252098293	35.65689978815134	17.030662542756712	13.902395148109017
7	28.60480046855817	32.040432450871386	21.067578896217263	18.28718818435318
8	26.092364430956778	35.885742421633765	19.601205523007437	18.420687624402028
9	26.460350054723204	16.482931219965394	19.146137822663352	37.91058090264805
10-11	25.067709208259238	26.478789704338602	27.37603325732138	21.07746783008078
12-13	25.621307259502608	23.85072277062987	27.344905301390703	23.183064668476824
14-15	23.054246924303126	25.21007064758381	24.679155833216544	27.056526594896518
16-17	23.24543986530539	27.958866268873045	25.579827860184835	23.21586600563673
18-19	23.240872126016736	27.28522467257624	26.97576921795926	22.49813398344776
20-21	24.04254545431141	26.54582310776255	26.712666449121876	22.698964988804164
22-23	23.650088374117363	26.29440930128535	26.403901494033438	23.651600830563847
24-25	23.33828800808881	26.519202073175798	26.5301755139094	23.612334404825997
26-27	23.30033523705934	26.59196573454084	26.567363984421572	23.540335043978242
28-29	23.378423627359123	26.750420725283885	26.60444807874285	23.26670756861414
30-31	23.67154468876121	26.67382030529593	26.360820457668755	23.29381454827411
32-33	23.211867071143022	26.685093789108294	26.44559697537326	23.657442164375425
34-35	23.429485673534092	26.701704294946843	26.552942948374262	23.315867083144802
36-37	23.650761289321803	26.554009052665826	26.38715795242853	23.40807170558384
38-39	23.549825398033434	26.632805539764888	26.38078594778787	23.436583114413807
40-41	23.58797203224771	26.59752229077484	26.480901494203955	23.33360418277349
42-43	23.545573102082816	26.60359249256425	26.280787880104363	23.570046525248575
44-45	23.615945947728896	26.579459667151568	26.295132389737702	23.509461995381834
46-47	23.51641762124378	26.61761773537943	26.38495289168588	23.48101175169091
48-49	23.459988147782955	26.692412441062736	26.233030420100434	23.61456899105388
50-51	23.362568808284287	26.853012245171193	26.194747398287323	23.5896715482572
52-53	23.55035928566682	26.64543406558132	26.065511810111424	23.738694838640438
54-55	23.45529813764833	26.700684896572042	26.009320205518847	23.83469676026078
56-57	23.44614101386448	26.69198246654795	26.030495772306473	23.831380747281095
58-59	23.50308289857796	26.446809339160037	26.152180846219373	23.897926916042625
60-61	23.59136941696952	26.48336034030881	26.254625658541404	23.670644584180266
62-63	23.30783656363577	26.465334766996946	26.39282554564557	23.834003123721715
64-65	23.22735250579987	26.436096507397462	26.278397319242536	24.058153667560134
66-67	23.38748254053709	26.593410801512636	26.066791083859965	23.95231557409031
68-69	23.545077410400143	26.288175416159948	26.173674510418522	23.99307266302139
70-71	23.35134373215433	26.19257143859368	26.298142980329192	24.157941848922796
72-73	23.406538658726273	26.153009621997775	26.28834260066537	24.152109118610586
74-75	23.30925120792768	26.283547356444902	26.26237272089079	24.14482871473663
76-77	23.407555149871627	26.162333534298128	26.30783590524824	24.122275410582013
78-79	23.328040824614476	26.249261070745096	26.327892475616544	24.094805629023885
80-81	23.37436170563678	26.171665884931222	26.344285943002234	24.109686466429757
82-83	23.338952005087055	26.15576979021051	26.36444262518761	24.140835579514825
84-85	23.391568127049776	26.212640263182074	26.16440223053435	24.2313893792338
86-87	23.270090875194537	26.095556141675253	26.3222171030621	24.31213588006811
88-89	23.274536878969556	25.92779472749816	26.4175524374935	24.38011595603878
90-91	23.157293481811145	26.095667466335858	26.42636366122988	24.320675390623116
92-93	23.209336424620272	26.01588940367427	26.51347117108883	24.26130300061663
94-95	23.126606400968303	26.00539601077529	26.570211941542087	24.29778564671432
96-97	23.1918701981282	25.858745400468948	26.481391731045616	24.467992670357233
98-99	23.11700586096075	25.890849622093054	26.377251761940666	24.61489275500553
100-101	23.017506174385204	25.969447273639002	26.471991617609262	24.541054934366528
102-103	22.82071037185649	25.90532730618542	26.611508994700824	24.662453327257268
104-105	22.753684917860216	26.064957795278175	26.441330231170557	24.74002705569105
106-107	22.59921964831872	26.090187025305507	26.52043454436168	24.790158782014093
108-109	22.639412047919794	26.080509347346414	26.561039678268994	24.719038926464794
110-111	22.550522448458036	26.241321024448627	26.431368118598144	24.776788408495197
112-113	22.555168476103542	26.28455054186564	26.420175844665344	24.74010513736547
114-115	22.491646384756155	26.337543988840817	26.394865463505756	24.775944162897268
116-117	22.488517080164417	26.26141553889067	26.395260243311586	24.854807137633323
118-119	22.53850760755146	26.097237119586225	26.4014490696804	24.962806203181916
120-121	22.436368311606643	26.161704631049087	26.455804625047048	24.946122432297223
122-123	22.41973028823301	26.1632418191947	26.436410026509034	24.98061786606326
124-125	22.29537238214585	26.185254756602834	26.308202620397786	25.21117024085353
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1266.0
1	881.0
2	421.5
3	311.5
4	252.5
5	211.5
6	222.5
7	292.0
8	351.5
9	372.0
10	388.5
11	436.0
12	505.0
13	578.5
14	662.5
15	776.5
16	868.5
17	980.5
18	1209.0
19	1466.0
20	1758.0
21	2187.5
22	2883.5
23	3693.5
24	4736.5
25	6380.5
26	8864.0
27	12138.0
28	15325.5
29	18979.5
30	24022.0
31	30647.5
32	37739.5
33	43269.5
34	47860.5
35	53406.0
36	60157.5
37	67023.5
38	72645.0
39	77954.5
40	83982.5
41	89740.5
42	93882.0
43	98827.5
44	105151.0
45	110633.0
46	115228.5
47	119003.5
48	122594.0
49	125976.0
50	131157.0
51	132155.0
52	127351.0
53	124447.5
54	121718.0
55	116062.5
56	107692.0
57	98823.0
58	91588.5
59	82320.0
60	69521.0
61	57058.0
62	45696.5
63	34608.5
64	24992.0
65	18421.5
66	13941.5
67	10386.0
68	7709.0
69	5778.0
70	4475.0
71	3453.5
72	2618.5
73	2002.5
74	1481.5
75	1088.5
76	784.0
77	516.0
78	357.0
79	222.0
80	139.5
81	96.0
82	61.0
83	53.0
84	53.0
85	34.5
86	23.5
87	23.0
88	22.0
89	15.0
90	11.0
91	13.5
92	10.0
93	8.0
94	11.0
95	8.5
96	3.5
97	4.5
98	5.5
99	8.5
100	11.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.035526299276248195
2	0.018117125446130193
3	0.024359971514601342
4	0.021721242764010443
5	0.014319930414792069
6	0.004826942836446765
7	0.004891302074266055
8	0.006178486830651859
9	0.00321796189096451
10-11	0.00485912245535641
12-13	0.008945934056881339
14-15	0.0029122555113228815
16-17	0.002670908369500543
18-19	4.826942836446765E-5
20-21	0.00485912245535641
22-23	9.492987578345304E-4
24-25	0.0017376994211208354
26-27	0.0017537892305756581
28-29	0.00403854217316046
30-31	0.0015285318982081425
32-33	0.0
34-35	0.007433491968128018
36-37	0.009509077387800127
38-39	0.0019468669440335287
40-41	0.0015928911360274323
42-43	0.003362770176057913
44-45	0.0014963522792984971
46-47	0.02341067275676681
48-49	0.0039098236975218794
50-51	0.012002997853297623
52-53	0.002300842752039625
54-55	2.4134714182233826E-4
56-57	3.7006561746091865E-4
58-59	0.008173623203049856
60-61	8.527599011055952E-4
62-63	0.011777740520930106
64-65	0.012083446900571735
66-67	4.826942836446765E-5
68-69	0.005084379787723926
70-71	0.005969319307739166
72-73	0.013064925277315911
74-75	0.0021077650385817543
76-77	0.0015285318982081425
78-79	6.43592378192902E-4
80-81	0.0049717511215401685
82-83	0.001962956753488351
84-85	0.0016250707549370776
86-87	0.015880641931909856
88-89	0.0
90-91	7.079516160121922E-4
92-93	0.010587094621273238
94-95	0.008414970344872194
96-97	0.005631433309187892
98-99	0.0014802624698436746
100-101	0.018261933731223593
102-103	0.009106832151429564
104-105	0.00844714996378184
106-107	0.020096172009073366
108-109	0.011745560902020462
110-111	0.02238092495165817
112-113	0.003169692462600042
114-115	0.016701222214105808
116-117	0.007224324445215325
118-119	0.005921049879374698
120-121	0.00902638310415545
122-123	0.010104400337628562
124-125	0.056925745851162175
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	3107557.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.47418372396452
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.01932572731697	33.24110333806762
2	12.864770541334488	12.214889604919202
3	5.959501394433417	8.487673923076645
4	3.3199032064883496	6.304387790824275
5	2.0109184361606847	4.773335564609989
6	1.3501954929331212	3.8459657336865507
7	0.9328635472886317	3.1000854803358835
8	0.6822135335042465	2.59100245028445
9	0.49465878226912524	2.113516971910532
>10	2.2531119968784403	17.860863990819038
>50	0.08510422232181182	2.7211608978999755
>100	0.02613312602769304	2.0249427308201504
>500	7.526275518159387E-4	0.23952346563549967
>1k	5.473654912266502E-4	0.4815480571102715
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	3252	0.10464812069416586	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	3.21796189096451E-5
12-13	0.0	0.0	0.0	0.0	1.608980945482255E-4
14-15	0.0	0.0	0.0	0.0	1.7698790400304807E-4
16-17	0.0	0.0	0.0	0.0	2.252573323675157E-4
18-19	0.0	0.0	0.0	1.608980945482255E-5	2.896165701868059E-4
20-21	0.0	0.0	0.0	1.287184756385804E-4	3.378859985512736E-4
22-23	0.0	0.0	0.0	5.631433309187892E-4	3.861554269157412E-4
24-25	0.0	0.0	0.0	0.001399813422569562	4.1833504582538633E-4
26-27	0.0	0.0	0.0	0.0023491121804040923	6.918618065573697E-4
28-29	1.608980945482255E-5	0.0	0.0	0.0051970084539076835	7.401312349218373E-4
30-31	3.21796189096451E-5	0.0	0.0	0.013901595368966683	7.723108538314824E-4
32-33	3.21796189096451E-5	0.0	0.0	0.032243978147464394	8.366700916507727E-4
34-35	3.21796189096451E-5	0.0	0.0	0.05933921726938557	8.688497105604177E-4
36-37	3.21796189096451E-5	0.0	0.0	0.10220246965703284	8.688497105604177E-4
38-39	3.21796189096451E-5	0.0	0.0	0.17214487135714646	9.332089483797079E-4
40-41	3.21796189096451E-5	0.0	0.0	0.25323751100945213	9.332089483797079E-4
42-43	3.21796189096451E-5	0.0	0.0	0.3252233185103282	9.332089483797079E-4
44-45	3.21796189096451E-5	0.0	0.0	0.4077318613946582	9.65388567289353E-4
46-47	3.21796189096451E-5	0.0	0.0	0.5085345176291215	9.975681861989982E-4
48-49	4.826942836446766E-5	0.0	0.0	0.6124585968978203	0.0010136579956538207
50-51	6.43592378192902E-5	0.0	0.0	0.7257308554597712	0.0011262866618375784
52-53	6.43592378192902E-5	0.0	0.0	0.8359299604158508	0.0011906458996568687
54-55	6.43592378192902E-5	0.0	0.0	0.9596284155045265	0.0011906458996568687
56-57	6.43592378192902E-5	0.0	0.0	1.1018784208946126	0.0012710949469309815
58-59	6.43592378192902E-5	0.0	0.0	1.2565658489932767	0.0013515439942050943
60-61	6.43592378192902E-5	0.0	0.0	1.4242377533219825	0.0013837236131147394
62-63	6.43592378192902E-5	0.0	0.0	1.593293381263803	0.0014159032320243843
64-65	6.43592378192902E-5	0.0	0.0	1.7727269363039841	0.0014480828509340295
66-67	6.43592378192902E-5	0.0	0.0	1.979915412653734	0.0014802624698436746
68-69	6.43592378192902E-5	0.0	0.0	2.2142473975537698	0.0014802624698436746
70-71	9.65388567289353E-5	0.0	0.0	2.475288466148811	0.0014963522792984971
72-73	9.65388567289353E-5	0.0	0.0	2.7349136315118274	0.001673340183301545
74-75	9.65388567289353E-5	0.0	0.0	3.004530568546289	0.0018342382778497707
76-77	9.65388567289353E-5	0.0	0.0	3.2973329210051494	0.0018664178967594158
78-79	9.65388567289353E-5	0.0	0.0	3.6273510027330147	0.0018664178967594158
80-81	9.65388567289353E-5	0.0	0.0	3.9810211043594697	0.0019146873251238835
82-83	9.65388567289353E-5	0.0	0.0	4.3497190880167285	0.0020273159913076415
84-85	9.65388567289353E-5	0.0	0.0	4.725721845166476	0.0020594956102172866
86-87	9.65388567289353E-5	1.608980945482255E-5	0.0	5.126325920972648	0.002075585419672109
88-89	9.65388567289353E-5	3.21796189096451E-5	0.0	5.576068274853848	0.002123854848036577
90-91	9.65388567289353E-5	3.21796189096451E-5	0.0	6.050234959487469	0.002156034466946222
92-93	9.65388567289353E-5	3.21796189096451E-5	0.0	6.545768911077094	0.0023008427520396246
94-95	9.65388567289353E-5	3.21796189096451E-5	0.0	7.052372651571637	0.0023330223709492697
96-97	9.65388567289353E-5	3.21796189096451E-5	0.0	7.574905303426453	0.0025582797033167856
98-99	9.65388567289353E-5	3.21796189096451E-5	0.0	8.147268095162856	0.0027030879884101886
100-101	9.65388567289353E-5	3.21796189096451E-5	0.0	8.76577646041569	0.0027030879884101886
102-103	9.65388567289353E-5	4.826942836446766E-5	0.0	9.399731042745152	0.0027352676073198337
104-105	9.65388567289353E-5	6.43592378192902E-5	0.0	10.03096644727675	0.002767447226229479
106-107	9.65388567289353E-5	6.43592378192902E-5	0.0	10.67251541966889	0.002767447226229479
108-109	9.65388567289353E-5	6.43592378192902E-5	0.0	11.347386387441968	0.0027996268451391236
110-111	9.65388567289353E-5	6.43592378192902E-5	0.0	12.06034193419461	0.0028318064640487687
112-113	9.65388567289353E-5	6.43592378192902E-5	0.0	12.810593659263532	0.0028318064640487687
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATGGGG	7715	0.0	27.223791	4
GTACATG	14355	0.0	26.53545	1
GTATCAA	9680	0.0	26.069948	1
TACATGG	14355	0.0	25.40903	2
ACATGGG	14300	0.0	24.549734	3
TATCAAC	10770	0.0	22.706854	2
AACGCAG	10610	0.0	22.654156	6
TCAACGC	10685	0.0	22.552275	4
CAACGCA	10735	0.0	22.445791	5
ATCAACG	11020	0.0	22.029743	3
ACGCAGA	11725	0.0	20.34761	7
CGCAGAG	11815	0.0	19.99119	8
GGTATCA	7360	0.0	19.327236	1
ACCGTCG	220	1.0228161E-4	18.930288	8
ATGGGGA	4330	0.0	17.312689	5
GCAGAGT	12955	0.0	17.037992	9
ATGGGGG	4470	0.0	16.770458	5
TATACCG	355	8.110619E-7	16.75921	5
ATGGGGC	3390	0.0	16.146189	5
GTCTAGA	1835	0.0	15.89314	1
>>END_MODULE