FastQCFastQC Report
Mon 15 Aug 2016
SRR2921003_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921003_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3107557
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41180.1325156706699185No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT32140.10342529517559935No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA118250.033.906341
GGTATCA84300.028.6923071
TATCAAC142800.027.6892322
TCAACGC142850.027.3100854
ATCAACG144800.027.02373
CAACGCA143800.026.9762745
AACGCAG145800.026.6436066
ACGCAGA161550.023.7537427
CGCAGAG164650.023.0911948
CATGGGG75650.021.7301224
GTACATG142000.021.6904811
AGTACTT87300.021.19836612-13
TACATGG143400.020.929192
GAGTACT81300.020.82322312-13
GCAGAGT179650.020.0374479
ACATGGG142100.020.0346533
ACTATAC9500.019.4350683
ACCGTCG1856.9600064E-419.2968658
AGAGTAC161600.019.29397410-11
GTACTTT90250.018.72523714-15
Produced by FastQC (version 0.11.5)