##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921003_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3107557
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.623911001471573	31.0	30.0	34.0	26.0	34.0
2	29.660316447936435	31.0	30.0	34.0	26.0	34.0
3	29.802489544037325	31.0	30.0	34.0	26.0	34.0
4	33.321385898955356	35.0	33.0	37.0	28.0	37.0
5	32.802404589843405	35.0	33.0	37.0	26.0	37.0
6	32.57468229866741	35.0	33.0	37.0	25.0	37.0
7	32.399276988322335	35.0	33.0	37.0	23.0	37.0
8	32.30829233381721	35.0	32.0	37.0	23.0	37.0
9	33.58450126578531	37.0	33.0	39.0	23.0	39.0
10-11	33.48046278797139	37.0	33.0	39.0	22.5	39.0
12-13	33.361474302804424	37.0	33.0	39.0	20.5	39.0
14-15	34.192286094832696	38.0	33.0	40.0	19.0	41.0
16-17	34.176108756814436	38.0	33.0	40.0	19.0	41.0
18-19	34.215602642204146	38.5	33.0	40.5	19.0	41.0
20-21	34.191956736433156	38.0	33.0	41.0	18.5	41.0
22-23	34.15819581105029	38.0	33.0	41.0	19.0	41.0
24-25	34.130512972087075	38.0	33.0	41.0	18.5	41.0
26-27	33.97965153977867	38.0	33.0	40.5	18.0	41.0
28-29	33.853417813414204	38.0	33.0	40.0	17.5	41.0
30-31	33.673653290993535	38.0	32.5	40.0	16.5	41.0
32-33	33.525765416370476	38.0	32.0	40.0	16.0	41.0
34-35	33.38766626002355	38.0	32.0	40.0	15.5	41.0
36-37	33.2311869098459	38.0	32.0	40.0	12.5	41.0
38-39	33.085556757285545	38.0	32.0	40.0	9.0	41.0
40-41	32.888408000239416	38.0	31.5	40.0	9.0	41.0
42-43	32.724708991661295	38.0	31.0	40.0	8.0	41.0
44-45	32.49033549505286	38.0	31.0	40.0	6.0	41.0
46-47	32.321897876692205	38.0	31.0	40.0	2.0	41.0
48-49	32.098813151295374	37.5	31.0	40.0	2.0	41.0
50-51	31.896222820691627	37.0	30.0	40.0	2.0	41.0
52-53	31.64904730629237	37.0	30.0	40.0	2.0	41.0
54-55	31.458679921237163	37.0	30.0	40.0	2.0	41.0
56-57	31.322503818916275	36.0	30.0	40.0	2.0	41.0
58-59	31.25639754958638	36.0	30.0	40.0	2.0	41.0
60-61	30.988709298011266	36.0	29.5	39.5	2.0	41.0
62-63	30.732877627023413	35.5	29.0	39.0	2.0	41.0
64-65	30.47000730799145	35.0	29.0	39.0	2.0	41.0
66-67	30.16289918415012	35.0	29.0	39.0	2.0	40.5
68-69	29.860946074360022	35.0	28.5	38.0	2.0	40.0
70-71	29.514818392711703	35.0	28.0	37.0	2.0	40.0
72-73	29.237861123705855	34.0	28.0	37.0	2.0	39.0
74-75	28.975431182758676	34.0	28.0	36.0	2.0	39.0
76-77	28.649081899382697	34.0	28.0	36.0	2.0	39.0
78-79	28.32835761339213	34.0	27.0	35.5	2.0	37.5
80-81	28.075046893749658	34.0	27.0	35.0	2.0	37.0
82-83	27.82012526238457	34.0	26.0	35.0	2.0	36.5
84-85	27.619716388146703	34.0	26.0	35.0	2.0	36.0
86-87	27.392013726538245	34.0	26.0	35.0	2.0	36.0
88-89	27.26256654342945	34.0	26.0	35.0	2.0	35.5
90-91	27.069038637103034	33.0	25.5	35.0	2.0	35.0
92-93	27.204239857869062	34.0	26.0	35.0	2.0	35.0
94-95	27.146082919798417	34.0	26.0	35.0	2.0	35.0
96-97	27.08122007094319	34.0	26.0	35.0	2.0	35.0
98-99	27.030371928817395	34.0	25.5	35.0	2.0	35.0
100-101	26.895064354410877	34.0	25.0	35.0	2.0	35.0
102-103	25.609779804521686	31.5	23.5	34.0	2.0	35.0
104-105	26.65724602959817	33.0	25.0	35.0	2.0	35.0
106-107	26.84839006975576	34.0	25.0	35.0	2.0	35.0
108-109	26.826139311362592	34.0	25.0	35.0	2.0	35.0
110-111	26.76141097331441	34.0	25.0	35.0	2.0	35.0
112-113	26.652482641509067	34.0	25.0	35.0	2.0	35.0
114-115	26.55810963403085	34.0	25.0	35.0	2.0	35.0
116-117	26.420834275928	34.0	25.0	35.0	2.0	35.0
118-119	26.282447755584208	34.0	24.5	35.0	2.0	35.0
120-121	26.13691993421199	34.0	24.0	35.0	2.0	35.0
122-123	25.89080409466343	34.0	23.5	35.0	2.0	35.0
124-125	24.787087413038602	32.5	17.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	131493.0
3	39061.0
4	18713.0
5	14792.0
6	16559.0
7	19385.0
8	21192.0
9	22820.0
10	23899.0
11	24763.0
12	25476.0
13	27431.0
14	29339.0
15	24385.0
16	18449.0
17	16966.0
18	15493.0
19	14507.0
20	14561.0
21	15215.0
22	17360.0
23	22945.0
24	30492.0
25	40526.0
26	48847.0
27	57250.0
28	67494.0
29	79106.0
30	92580.0
31	110746.0
32	133339.0
33	165148.0
34	210570.0
35	284750.0
36	447828.0
37	585244.0
38	178514.0
39	319.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.02435401211454	19.199953123259426	13.410450293591344	25.36524257103468
2	15.974809778105294	20.7244280471303	38.233917018363314	25.06684515640109
3	19.063811957879615	24.66762392863142	29.307501848768485	26.961062264720475
4	13.392618368694922	16.85979411338263	35.35024890048813	34.39733861743431
5	13.875791970620845	37.46842980207373	34.21016973155475	14.445608495750676
6	33.26227837966387	36.16767939284057	16.603013270671102	13.967028956824462
7	28.33766657559295	32.04346565410718	21.16163717712679	18.457230593173076
8	26.294353346314146	35.77995912653074	19.479136829570507	18.446550697584605
9	26.475459148150904	15.751621427010276	19.226749353086916	38.54617007175191
10-11	25.29982001143275	26.12430090277528	27.44263990956798	21.133239176224
12-13	26.097772542127938	23.287596498766458	27.663848749800845	22.95078220930476
14-15	23.25606435858693	24.654074526635924	25.154091587120814	26.935769527656333
16-17	23.14156993668803	27.54546468618699	26.02087799749186	23.292087379633127
18-19	23.139756345844418	26.58365221108967	27.879734956406626	22.39685648665929
20-21	23.90722531808053	25.780930392595174	27.718011779818653	22.593832509505642
22-23	23.482064485644088	25.632304815710473	27.426532194701313	23.45909850394413
24-25	23.194028025215292	25.94371460436552	27.521327906606217	23.34092946381297
26-27	23.125719168878152	26.03158790878967	27.636652145155033	23.20604077717714
28-29	23.19611237872507	26.072963065602323	27.704242220589293	23.02668233508332
30-31	23.509613225844298	25.982530537279402	27.44457968822901	23.063276548647284
32-33	23.152938798343992	26.030354589657474	27.459599207659718	23.357107404338816
34-35	23.305721649310314	26.082780973577023	27.554665549473693	23.05683182763897
36-37	23.567333744724426	25.90358769728183	27.4086900090873	23.12038854890645
38-39	23.509350565028722	25.99241563760905	27.300276337841968	23.197957459520264
40-41	23.47215209453018	25.980154430969378	27.366485682227854	23.18120779227259
42-43	23.566886157101845	25.891389173103548	27.144031403694207	23.397693266100404
44-45	23.585154674218774	25.96056967856115	27.079447781074705	23.374827866145367
46-47	23.570450361817983	26.014822336071248	26.984912392982167	23.429814909128606
48-49	23.538395819840048	26.19236088130504	26.732762827685576	23.536480471169334
50-51	23.477856987437896	26.38705241785449	26.610948099719195	23.524142494988418
52-53	23.60119946738266	26.27667935332027	26.456300092820147	23.665821086476925
54-55	23.506578288733937	26.307956170708774	26.414329663509527	23.771135877047758
56-57	23.47898915954224	26.329081450774066	26.385378377956453	23.80655101172724
58-59	23.441163556955296	26.156645889477026	26.60319344675164	23.798997106816042
60-61	23.528705388230286	26.283602996498246	26.62246201820319	23.565229597068278
62-63	23.302878451569363	26.24158619095362	26.69626833522972	23.759267022247293
64-65	23.19359024487141	26.20301625119662	26.67522605625856	23.928167447673406
66-67	23.33819553117768	26.43677624371555	26.454450134356982	23.770578090749794
68-69	23.45898378433362	26.121255928316362	26.538062820879905	23.88169746647012
70-71	23.30437086425293	26.034883408369847	26.620388739098434	24.040356988278788
72-73	23.419495293781516	26.013722278783586	26.51875042756367	24.048031999871228
74-75	23.325190346803033	26.143847125795062	26.432459374522715	24.098503152879193
76-77	23.367297640823345	26.012532150327868	26.543207691037562	24.076962517811225
78-79	23.298007642633028	26.108820767669044	26.521581537675104	24.071590052022827
80-81	23.314175687165417	26.12893501973837	26.458441598362796	24.098447694733416
82-83	23.237940806542014	26.112030204199627	26.472681688285476	24.177347300972887
84-85	23.279194648369987	26.164513344876582	26.233811462449385	24.322480544304042
86-87	23.217447050902628	26.08240498279306	26.3421387207039	24.358009245600403
88-89	23.19917900434963	25.915314865223316	26.424268430554015	24.46123769987304
90-91	23.111056099209424	26.08982717445943	26.38146715561445	24.417649570716698
92-93	23.074489112235895	26.055843296153718	26.48654486121901	24.383122730391378
94-95	22.98232948040323	25.996254039106958	26.56788483890083	24.453531641588985
96-97	23.04842524962709	25.881751260748164	26.44334080198984	24.62648268763491
98-99	23.00850830568099	25.850210945338333	26.341628123985068	24.799652624995613
100-101	22.883683547457387	25.989743031821515	26.440710372136174	24.685863048584928
102-103	22.77587253599877	25.85655407106212	26.48227953469897	24.88529385824014
104-105	22.736536318243814	26.03092472408757	26.287415657100095	24.945123300568522
106-107	22.57829102637095	26.009955286179075	26.36768516423551	25.044068523214463
108-109	22.6155801808613	26.018046863395533	26.39893284977485	24.967440105968315
110-111	22.524418374288523	26.149720752387363	26.26646352287323	25.059397350450887
112-113	22.433576574913737	26.19431337048766	26.234414679396313	25.137695375202295
114-115	22.39362959560909	26.318390514749414	26.15392612072749	25.13405376891401
116-117	22.33006865259585	26.244386634065993	26.184217852507153	25.24132686083101
118-119	22.36995798086072	26.14274986004616	26.193147724798692	25.29414443429443
120-121	22.2638760028204	26.215299735048486	26.24085196409429	25.279972298036824
122-123	22.197510836529037	26.297877393517698	26.128219481729193	25.376392288224075
124-125	22.01548435463178	26.408611264252922	26.04739338324325	25.528510997872043
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	105.0
1	104.5
2	200.0
3	353.0
4	311.0
5	220.5
6	294.0
7	560.5
8	844.5
9	892.5
10	725.0
11	594.5
12	649.5
13	735.5
14	807.5
15	959.0
16	1143.0
17	1325.5
18	1557.5
19	1755.0
20	2043.5
21	2590.5
22	3308.0
23	4160.0
24	5261.0
25	6900.0
26	9408.5
27	12731.0
28	15988.0
29	19804.5
30	24732.5
31	31269.0
32	38464.0
33	43847.0
34	48297.5
35	53597.0
36	59985.0
37	66429.5
38	71939.5
39	77343.0
40	83830.0
41	89351.5
42	92968.0
43	98157.5
44	105072.5
45	111056.5
46	115299.0
47	119542.0
48	123622.5
49	126917.5
50	132633.5
51	132999.5
52	127506.0
53	124896.0
54	122336.0
55	116308.0
56	107363.5
57	98710.0
58	91795.5
59	82110.0
60	68498.0
61	55965.0
62	44961.5
63	33406.5
64	23563.0
65	17310.5
66	12870.5
67	9434.0
68	6893.5
69	5143.5
70	3966.0
71	2971.5
72	2209.5
73	1749.5
74	1320.0
75	920.5
76	644.5
77	395.0
78	245.5
79	146.5
80	91.0
81	56.0
82	30.5
83	21.5
84	14.0
85	11.0
86	7.5
87	5.0
88	7.0
89	6.5
90	6.5
91	6.0
92	3.5
93	3.5
94	3.0
95	3.5
96	4.5
97	2.0
98	0.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04952443350194381
2	6.435923781929019E-5
3	0.002670908369500543
4	0.1257257710799834
5	0.0190503343945099
6	0.003314500747693445
7	0.005921049879374698
8	0.013097104896225557
9	0.02831806464048769
10-11	0.022300475904384054
12-13	0.020981111529088606
14-15	0.030988973009988235
16-17	0.04094856506252339
18-19	0.04347466514693053
20-21	0.023104966377125183
22-23	0.025357539700800336
24-25	0.022911888663667314
26-27	0.02162470390728151
28-29	0.021560344669462217
30-31	0.02643555693427345
32-33	0.026097670935722177
34-35	0.030940703581623766
36-37	0.03295192976347658
38-39	0.03325763614311821
40-41	0.03605726298825734
42-43	0.03357943233221466
44-45	0.030988973009988235
46-47	0.04180132496362898
48-49	0.03452873109004919
50-51	0.02423125303896276
52-53	0.03254968452710602
54-55	0.03378859985512736
56-57	0.026242479220815577
58-59	0.03040973986961462
60-61	0.04498710723568385
62-63	0.05312855081982406
64-65	0.045582430185512286
66-67	0.04118991220434573
68-69	0.03097288320053341
70-71	0.03798804012283604
72-73	0.041656516678535586
74-75	0.025534527604803386
76-77	0.02149598543164293
78-79	0.03333808519039232
80-81	0.025437988748074455
82-83	0.03970964973450205
84-85	0.022220026857109942
86-87	0.03560674832352231
88-89	0.034914886516964935
90-91	0.03728008850682385
92-93	0.028366334068852157
94-95	0.023941636468775954
96-97	0.01842283182577182
98-99	0.02664472445718614
100-101	0.025454078557529274
102-103	0.02970178825360243
104-105	0.026371197696454164
106-107	0.014931343174075327
108-109	0.019002064966145434
110-111	0.023378493137857167
112-113	0.013724607464963635
114-115	0.01613807888318702
116-117	0.014931343174075327
118-119	0.00909074234197474
120-121	0.006307205306290439
122-123	0.00410290141097975
124-125	0.07172837054959894
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	3107557.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.88135372206587
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.52697218660484	30.307019244871153
2	13.78343902667751	12.372388049256813
3	6.440183017717606	8.671323961590762
4	3.60517941606472	6.472213304156463
5	2.242918296178055	5.033260471023029
6	1.4601698661504476	3.9320640154199884
7	1.0020972486684976	3.148283675697989
8	0.7533214563327819	2.70480693984747
9	0.5419807825993752	2.189234809296418
>10	2.5140294907475003	18.926908501761822
>50	0.09573170163092465	2.8881135487030916
>100	0.03231301448600795	2.407126106381502
>500	0.0010131715649591157	0.285892175101821
>1k	6.513245766045623E-4	0.6613651968916344
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4118	0.1325156706699185	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	3214	0.10342529517559935	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.65388567289353E-5
2	0.0	0.0	0.0	0.0	9.65388567289353E-5
3	0.0	0.0	0.0	0.0	9.65388567289353E-5
4	0.0	0.0	0.0	0.0	9.65388567289353E-5
5	0.0	0.0	0.0	0.0	1.608980945482255E-4
6	0.0	0.0	0.0	0.0	1.608980945482255E-4
7	0.0	0.0	0.0	0.0	1.608980945482255E-4
8	0.0	0.0	0.0	0.0	1.608980945482255E-4
9	0.0	0.0	0.0	0.0	1.608980945482255E-4
10-11	0.0	0.0	0.0	0.0	1.7698790400304807E-4
12-13	0.0	0.0	0.0	0.0	3.700656174609187E-4
14-15	0.0	0.0	0.0	0.0	4.505146647350314E-4
16-17	0.0	0.0	0.0	0.0	5.470535214639667E-4
18-19	0.0	0.0	0.0	1.608980945482255E-5	6.275025687380795E-4
20-21	0.0	0.0	0.0	1.930777134578706E-4	6.43592378192902E-4
22-23	0.0	0.0	0.0	5.953229498284343E-4	6.43592378192902E-4
24-25	0.0	0.0	0.0	0.0013193643752954492	6.43592378192902E-4
26-27	0.0	0.0	0.0	0.0022364835142203348	7.079516160121922E-4
28-29	0.0	0.0	0.0	0.005068289978269103	7.562210443766599E-4
30-31	0.0	1.608980945482255E-5	0.0	0.013515439942050943	7.723108538314824E-4
32-33	0.0	3.21796189096451E-5	0.0	0.03160038576927149	8.044904727411275E-4
34-35	0.0	3.21796189096451E-5	0.0	0.05808421213190941	8.688497105604177E-4
36-37	0.0	3.21796189096451E-5	0.0	0.10048086004536683	8.849395200152402E-4
38-39	0.0	3.21796189096451E-5	0.0	0.16911998717963983	9.814783767441756E-4
40-41	0.0	3.21796189096451E-5	0.0	0.24911851978901756	9.975681861989982E-4
42-43	0.0	3.21796189096451E-5	0.0	0.3197849629145982	9.975681861989982E-4
44-45	0.0	3.21796189096451E-5	0.0	0.400587985996717	0.0010136579956538207
46-47	0.0	3.21796189096451E-5	0.0	0.5002643555693427	0.0010619274240182882
48-49	0.0	3.21796189096451E-5	0.0	0.6024990048452852	0.0011584662807472236
50-51	0.0	3.21796189096451E-5	0.0	0.7134382410362867	0.001287184756385804
52-53	0.0	3.21796189096451E-5	0.0	0.8223501612359805	0.0013193643752954492
54-55	0.0	3.21796189096451E-5	0.0	0.9448257908060897	0.0013515439942050943
56-57	0.0	3.21796189096451E-5	0.0	1.0871240656245404	0.0013515439942050943
58-59	0.0	3.21796189096451E-5	0.0	1.2416345058192013	0.0013515439942050943
60-61	0.0	3.21796189096451E-5	0.0	1.4066355017784067	0.0013515439942050943
62-63	0.0	4.826942836446766E-5	0.0	1.5744843940111153	0.0014802624698436746
64-65	0.0	6.43592378192902E-5	0.0	1.7523894171530885	0.0015607115171177874
66-67	0.0	6.43592378192902E-5	0.0	1.9589503909341004	0.0015928911360274325
68-69	0.0	6.43592378192902E-5	0.0	2.1929605796450398	0.0016250707549370776
70-71	0.0	6.43592378192902E-5	0.0	2.452489206151327	0.0016572503738467228
72-73	0.0	6.43592378192902E-5	0.0	2.7118891141819765	0.001785968849485303
74-75	0.0	6.43592378192902E-5	0.0	2.9803958543640547	0.001930777134578706
76-77	0.0	6.43592378192902E-5	0.0	3.2724902552069035	0.0019951363723979963
78-79	0.0	6.43592378192902E-5	0.0	3.602009552841669	0.0020594956102172866
80-81	0.0	6.43592378192902E-5	0.0	3.955969271038311	0.0020594956102172866
82-83	0.0	6.43592378192902E-5	0.0	4.323894943841738	0.002123854848036577
84-85	0.0	6.43592378192902E-5	0.0	4.6979669238569075	0.002156034466946222
86-87	0.0	6.43592378192902E-5	0.0	5.0942106613008225	0.002252573323675157
88-89	0.0	6.43592378192902E-5	0.0	5.5403971672925065	0.0023330223709492697
90-91	0.0	6.43592378192902E-5	0.0	6.014596031545037	0.0023491121804040923
92-93	3.21796189096451E-5	6.43592378192902E-5	0.0	6.50942203151865	0.0024778306560426728
94-95	3.21796189096451E-5	6.43592378192902E-5	0.0	7.013531851547695	0.002510010274952318
96-97	3.21796189096451E-5	6.43592378192902E-5	0.0	7.536048413593058	0.0028961657018680594
98-99	3.21796189096451E-5	6.43592378192902E-5	0.0	8.110406341701857	0.00315360265314522
100-101	3.21796189096451E-5	6.43592378192902E-5	0.0	8.730024903807074	0.00315360265314522
102-103	3.21796189096451E-5	6.43592378192902E-5	0.0	9.365926353080571	0.003185782272054865
104-105	3.21796189096451E-5	6.43592378192902E-5	0.0	9.999382151316935	0.00321796189096451
106-107	3.21796189096451E-5	6.43592378192902E-5	0.0	10.641622985515632	0.00321796189096451
108-109	3.21796189096451E-5	6.43592378192902E-5	0.0	11.320709483365873	0.00321796189096451
110-111	3.21796189096451E-5	6.43592378192902E-5	0.0	12.038781589525147	0.0032501415098741553
112-113	3.21796189096451E-5	6.43592378192902E-5	1.608980945482255E-5	12.79508308294908	0.0032501415098741553
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	11825	0.0	33.90634	1
GGTATCA	8430	0.0	28.692307	1
TATCAAC	14280	0.0	27.689232	2
TCAACGC	14285	0.0	27.310085	4
ATCAACG	14480	0.0	27.0237	3
CAACGCA	14380	0.0	26.976274	5
AACGCAG	14580	0.0	26.643606	6
ACGCAGA	16155	0.0	23.753742	7
CGCAGAG	16465	0.0	23.091194	8
CATGGGG	7565	0.0	21.730122	4
GTACATG	14200	0.0	21.690481	1
AGTACTT	8730	0.0	21.198366	12-13
TACATGG	14340	0.0	20.92919	2
GAGTACT	8130	0.0	20.823223	12-13
GCAGAGT	17965	0.0	20.037447	9
ACATGGG	14210	0.0	20.034653	3
ACTATAC	950	0.0	19.435068	3
ACCGTCG	185	6.9600064E-4	19.296865	8
AGAGTAC	16160	0.0	19.293974	10-11
GTACTTT	9025	0.0	18.725237	14-15
>>END_MODULE