FastQCFastQC Report
Mon 15 Aug 2016
SRR2921002_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921002_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2507282
Sequences flagged as poor quality0
Sequence length125
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA25480.10162398964296795No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG122850.027.9517441
CATGGGG70100.027.9281924
GTATCAA75550.026.5462821
TACATGG126800.025.9525172
ACATGGG125750.025.3174153
GGTATCA59500.022.0046251
TCAACGC87650.021.9967274
CAACGCA88350.021.8224455
TATCAAC90850.021.4188632
AACGCAG90450.021.3777396
ATCAACG88650.021.2791423
ATGGGGA41700.020.6917345
GGACCGT5001.8189894E-1219.0386856
ACGCAGA101800.018.9942677
CCTACAC15050.018.979223
CGCAGAG103100.018.697068
GTATAAC6850.017.3759161
GTATAGG5951.8189894E-1217.0035741
CTACACT13050.016.8715974
GTCTTAC8050.016.2642881
Produced by FastQC (version 0.11.5)