FastQCFastQC Report
Mon 15 Aug 2016
SRR2921002_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921002_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2507282
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33130.13213511683169266No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA25690.10246154999716825No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT25470.10158410581657747No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25240.10066677780959622No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA94550.035.8549271
TCAACGC117300.028.4906434
CAACGCA117950.028.3002825
ATCAACG118900.028.156793
TATCAAC119500.028.0602252
AACGCAG121900.027.7205076
GGTATCA65950.027.4635641
CATGGGG73350.026.0700724
GTACATG128000.025.6471841
TACATGG131600.024.4845432
ACGCAGA134950.024.4677457
ACATGGG127650.024.360063
CGCAGAG139000.023.6269048
AGTACTT64450.022.48073812-13
GAGTACT58900.021.9724512-13
GCAGAGT152150.020.7645459
AGAGTAC135950.020.08884410-11
TACTTTT72950.019.61662514-15
ATGGGGA39150.019.6057645
GTACTTT66650.019.37287514-15
Produced by FastQC (version 0.11.5)