FastQCFastQC Report
Mon 15 Aug 2016
SRR2921001_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921001_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3454970
Sequences flagged as poor quality0
Sequence length125
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA60900.040.754011
ATCAACG69800.035.125133
CAACGCA71100.034.0609635
TCAACGC71000.033.9423074
AACGCAG72800.033.4280856
TATCAAC75900.032.0669482
GGTATCA40300.030.2761331
GTACATG120500.030.2284551
TACATGG121700.028.8494532
ACGCAGA85050.028.5433797
ACATGGG118450.028.3850383
CGCAGAG85500.027.9056118
CATGGGG83550.025.2116624
GCAGAGT100250.022.4347239
AGAGTAC86500.019.56920410-11
ATGGGGA53600.019.4269875
GTGTAGC22800.019.317391
GTATAAT19400.019.021361
CAGAGTA94850.017.6896310-11
GTCTACG3757.48405E-817.4587461
Produced by FastQC (version 0.11.5)