FastQCFastQC Report
Mon 15 Aug 2016
SRR2921001_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921001_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3454970
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA76100.041.506891
GGTATCA47600.038.9387861
ATCAACG97650.032.0282673
TCAACGC97350.032.0055054
CAACGCA96900.031.9861985
TATCAAC100150.031.46352
AACGCAG101450.030.7257696
ACGCAGA115450.026.5906937
CGCAGAG119050.025.787358
GTACATG120000.025.0806671
TACATGG121000.023.8266122
ACATGGG116850.023.5537263
GCAGAGT132450.021.9206929
CATGGGG82200.021.7424474
AGAGTAC115050.020.97023410-11
GTACCGT4152.910383E-1120.0679746
GAGTACT60050.018.87528812-13
AGTACTT67950.018.607212-13
CAGAGTA124850.018.37108810-11
GTACTTT64950.017.35967614-15
Produced by FastQC (version 0.11.5)