##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921001_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3454970
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.637563567845742	31.0	30.0	34.0	26.0	34.0
2	29.75110232505637	31.0	30.0	34.0	26.0	34.0
3	29.874091526120342	31.0	30.0	34.0	26.0	34.0
4	33.379777827303855	35.0	33.0	37.0	28.0	37.0
5	32.84868551680622	35.0	33.0	37.0	26.0	37.0
6	32.619346332963815	35.0	33.0	37.0	25.0	37.0
7	32.44928928471159	35.0	33.0	37.0	24.0	37.0
8	32.382257154186576	35.0	33.0	37.0	23.0	37.0
9	33.67301423746082	37.0	33.0	39.0	23.0	39.0
10-11	33.55038480797228	37.0	33.0	39.0	22.5	39.0
12-13	33.431919814064955	37.0	33.0	39.0	21.5	39.0
14-15	34.30076310358701	38.0	33.0	41.0	19.0	41.0
16-17	34.28633707383855	38.0	33.0	41.0	19.0	41.0
18-19	34.30677241770551	38.5	33.0	41.0	19.0	41.0
20-21	34.274687334477576	39.0	33.0	41.0	19.0	41.0
22-23	34.22424391528725	39.0	33.0	41.0	19.0	41.0
24-25	34.18820308135816	38.5	33.0	41.0	19.0	41.0
26-27	34.04662819069341	38.0	33.0	41.0	18.0	41.0
28-29	33.92870140695867	38.0	33.0	40.0	17.5	41.0
30-31	33.77027861312834	38.0	33.0	40.0	16.5	41.0
32-33	33.652812614870754	38.0	33.0	40.0	16.0	41.0
34-35	33.53065844276506	38.0	32.0	40.0	16.0	41.0
36-37	33.390125384590895	38.0	32.0	40.0	15.0	41.0
38-39	33.258813680003016	38.0	32.0	40.0	13.0	41.0
40-41	33.08863694909073	38.0	32.0	40.0	9.0	41.0
42-43	32.941125393274035	38.0	32.0	40.0	9.0	41.0
44-45	32.73326859567521	38.0	31.0	40.0	8.0	41.0
46-47	32.60923293111084	38.0	31.0	40.0	7.5	41.0
48-49	32.41668350231695	38.0	31.0	40.0	2.0	41.0
50-51	32.22566910855956	37.5	31.0	40.0	2.0	41.0
52-53	31.99381022121755	37.0	30.5	40.0	2.0	41.0
54-55	31.819041699349054	37.0	30.0	40.0	2.0	41.0
56-57	31.693187784553846	37.0	30.0	40.0	2.0	41.0
58-59	31.64319183668744	37.0	30.0	40.0	2.0	41.0
60-61	31.382812730646	36.0	30.0	40.0	2.0	41.0
62-63	31.142088353878613	36.0	30.0	39.0	2.0	41.0
64-65	30.883919107835958	35.0	29.0	39.0	2.0	41.0
66-67	30.581687684697698	35.0	29.0	39.0	2.0	41.0
68-69	30.283720408570844	35.0	29.0	38.5	2.0	40.0
70-71	29.938753737369645	35.0	29.0	37.5	2.0	40.0
72-73	29.655125370118988	35.0	29.0	37.0	2.0	39.5
74-75	29.38965577125127	34.5	29.0	37.0	2.0	39.0
76-77	29.052846479130064	34.0	28.0	36.0	2.0	39.0
78-79	28.723320462985207	34.0	28.0	36.0	2.0	38.0
80-81	28.45153416672214	34.0	28.0	35.0	2.0	37.0
82-83	28.192088064440505	34.0	27.0	35.0	2.0	37.0
84-85	27.98533619684107	34.0	27.0	35.0	2.0	36.0
86-87	27.74422918288726	34.0	26.0	35.0	2.0	36.0
88-89	27.606534789014084	34.0	26.0	35.0	2.0	36.0
90-91	27.418413039765902	34.0	26.0	35.0	2.0	35.0
92-93	27.55046281154395	34.0	27.0	35.0	2.0	35.0
94-95	27.48929353945186	34.0	26.5	35.0	2.0	35.0
96-97	27.41803980931817	34.0	27.0	35.0	2.0	35.0
98-99	27.364997670023186	34.0	26.5	35.0	2.0	35.0
100-101	27.231624442469833	34.0	26.0	35.0	2.0	35.0
102-103	25.95002431280156	31.5	24.0	34.0	2.0	35.0
104-105	26.998395355097152	33.5	25.0	35.0	2.0	35.0
106-107	27.19966714616914	34.0	26.0	35.0	2.0	35.0
108-109	27.178737442003836	34.0	26.0	35.0	2.0	35.0
110-111	27.12012217182783	34.0	26.0	35.0	2.0	35.0
112-113	27.018153269058775	34.0	25.0	35.0	2.0	35.0
114-115	26.9355194111671	34.0	25.0	35.0	2.0	35.0
116-117	26.804752718547483	34.0	25.0	35.0	2.0	35.0
118-119	26.67338457352741	34.0	25.0	35.0	2.0	35.0
120-121	26.535215356428566	34.0	25.0	35.0	2.0	35.0
122-123	26.29986816093917	34.0	25.0	35.0	2.0	35.0
124-125	25.19498837905973	32.5	21.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	145429.0
3	43921.0
4	20938.0
5	15748.0
6	16696.0
7	19293.0
8	21636.0
9	23378.0
10	24277.0
11	25095.0
12	25303.0
13	26267.0
14	27782.0
15	23992.0
16	19822.0
17	17603.0
18	16329.0
19	15607.0
20	15389.0
21	16282.0
22	19026.0
23	24685.0
24	33656.0
25	43559.0
26	53436.0
27	63010.0
28	74485.0
29	87633.0
30	102468.0
31	120788.0
32	147427.0
33	181527.0
34	232253.0
35	315616.0
36	494121.0
37	680903.0
38	219196.0
39	394.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.84094197872755	19.619807604485	13.789069208001045	25.750181208786405
2	15.622865392675129	20.571478520206266	38.955498285367625	24.850157801750985
3	18.847470146782662	24.644736872573013	29.24856956951626	27.259223411128065
4	13.296300793558563	17.088098244422298	35.88094332126124	33.734657640757895
5	14.040754366549614	37.258000504269646	34.34849201085772	14.352753118323022
6	32.86041813861714	36.40273780617173	17.05232854484508	13.684515510366053
7	28.483149840048817	31.740035035272623	21.234402877847774	18.54241224683079
8	25.807796225720374	36.3919776440191	19.52637115351327	18.273854976747256
9	26.06138227363499	16.273621093259205	19.469420320986895	38.19557631211891
10-11	25.342455541970445	26.250700746174356	27.552000685511775	20.854843026343424
12-13	25.939191628354813	23.218907764088346	27.815360271335056	23.02654033622179
14-15	22.88992665057911	24.72985786106893	25.233822088207493	27.14639340014447
16-17	23.245076035545747	27.77645956354932	25.835046086908807	23.14341831399612
18-19	23.150856060291293	26.862050389734254	27.481096719619064	22.50599683035539
20-21	23.95316106774307	26.169030031522304	27.158949464864797	22.718859435869827
22-23	23.54902158330033	25.953403324462847	27.022189527270367	23.47538556496645
24-25	23.33306071933907	26.20668242037802	27.15540807058049	23.30484878970243
26-27	23.23134902817017	26.465358541648754	27.004085104264103	23.299207325916978
28-29	23.228694423697117	26.47328226595566	27.195882765049532	23.10214054529769
30-31	23.44107158754273	26.303053375997266	27.01397096661892	23.241904069841084
32-33	23.28950050201094	26.283312507889157	26.938923160480215	23.488263829619687
34-35	23.43514175423013	26.376669057151897	26.9674817767723	23.22070741184567
36-37	23.538001388867375	26.159072362827633	26.973718211056596	23.329208037248396
38-39	23.398525999134304	26.354924121238092	26.981425157034224	23.265124722593384
40-41	23.535156546889947	26.242354919915854	26.93084824468078	23.291640288513417
42-43	23.58473303571713	26.124160330572295	26.8213599221052	23.46974671160538
44-45	23.470601979121337	26.35794249899463	26.695468414922363	23.475987106961668
46-47	23.52302473138501	26.36675253138782	26.539698064837058	23.570524672390114
48-49	23.520361514083426	26.39891856147135	26.55727495557219	23.52344496887303
50-51	23.45600935218516	26.548362180624785	26.455546722421563	23.54008174476849
52-53	23.649386830111084	26.417210303193926	26.358596719289572	23.57480614740542
54-55	23.49886396290562	26.386070209191516	26.427009526208472	23.688056301694395
56-57	23.45418488173475	26.576792796965925	26.39702093164645	23.57200138965288
58-59	23.386999411988317	26.36159736515178	26.574451225284907	23.676951997574992
60-61	23.552393538165038	26.37859216036914	26.559199127179735	23.509815174286086
62-63	23.415847533333622	26.461734926571197	26.521517543135715	23.60089999695947
64-65	23.31113651153986	26.270014600822773	26.545323719134316	23.87352516850305
66-67	23.314223337937104	26.548025445248413	26.406973411047314	23.730777805767165
68-69	23.3618199386936	26.328253419080262	26.55464557012417	23.75528107210197
70-71	23.306033051904894	26.228653815223137	26.523463847045075	23.941849285826898
72-73	23.435806806499432	26.311031696980464	26.437029724270918	23.81613177224919
74-75	23.340478407285428	26.37196710718306	26.332058337154844	23.955496148376668
76-77	23.36994235215817	26.16589018336547	26.466852852819816	23.99731461165654
78-79	23.451966233377075	26.201949766973243	26.458130820692805	23.887953178956877
80-81	23.24457224664151	26.398716316517433	26.386441129404066	23.970270307436984
82-83	23.35832256727028	26.36505089824	26.36923475151965	23.907391782970066
84-85	23.335417256826176	26.251255167047045	26.28046580104953	24.13286177507725
86-87	23.290483221865138	26.16131040273314	26.4588146732679	24.08939170213382
88-89	23.237621383116096	26.115392855695234	26.480077824166028	24.166907937022646
90-91	23.109840024968776	26.2843110743348	26.497402656811953	24.108446243884472
92-93	23.16615579480684	26.197962544105675	26.499419162218402	24.136462498869086
94-95	23.055023560185553	26.202837470545244	26.4952318723794	24.246907096889807
96-97	23.100814959485067	26.112385754340877	26.52353479032991	24.263264495844144
98-99	23.009353121369394	26.125279772887094	26.383129698527075	24.482237407216438
100-101	22.911109036310695	26.12604123014434	26.54788193034606	24.41496780319891
102-103	22.913591198353483	26.04103667516772	26.49926275614266	24.546109370336133
104-105	22.925709167269922	26.049682782384043	26.4007941694897	24.62381388085634
106-107	22.774181212744647	26.144844618692943	26.41821636910151	24.6627577994609
108-109	22.860050970139177	26.094166659067607	26.361885093267944	24.683897277525272
110-111	22.687828117588428	26.219415196010388	26.39733998674394	24.69541669965725
112-113	22.63142278187871	26.20176581270806	26.411029092488057	24.75578231292517
114-115	22.504418891840928	26.321238528684148	26.366672205358483	24.807670374116437
116-117	22.523379473768614	26.215839741834333	26.360201081132406	24.900579703264643
118-119	22.49358773778052	26.244424638949333	26.18145262017727	25.08053500309288
120-121	22.420869757515604	26.314230230066816	26.32236389071486	24.94253612170272
122-123	22.34257700519157	26.33356701570438	26.248613536056165	25.07524244304788
124-125	22.32950438344485	26.397686412247694	26.06897148907382	25.20383771523363
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	48.0
1	51.5
2	116.5
3	193.5
4	192.0
5	166.0
6	186.5
7	329.0
8	483.0
9	520.0
10	474.5
11	419.5
12	451.0
13	541.5
14	636.0
15	741.5
16	877.5
17	1034.5
18	1215.5
19	1437.5
20	1741.0
21	2218.0
22	2735.5
23	3453.5
24	4513.0
25	6007.5
26	8160.5
27	11080.5
28	14582.0
29	18855.5
30	24434.5
31	31627.5
32	39760.0
33	47231.5
34	54048.0
35	61654.0
36	70463.0
37	78693.0
38	85059.5
39	90529.0
40	97705.0
41	106059.0
42	113941.5
43	121862.5
44	127572.0
45	132274.0
46	136908.5
47	140981.0
48	146233.5
49	149200.0
50	147758.0
51	144289.0
52	140300.5
53	136588.5
54	130995.5
55	121744.5
56	110264.0
57	99164.0
58	89566.5
59	78983.5
60	67250.0
61	55538.5
62	44803.0
63	34544.5
64	25901.5
65	20079.5
66	15594.0
67	12249.0
68	9380.0
69	6982.0
70	5405.0
71	4429.0
72	3714.0
73	2973.5
74	2260.0
75	1668.5
76	1103.0
77	652.0
78	389.0
79	242.5
80	161.0
81	106.5
82	61.5
83	36.5
84	24.0
85	19.5
86	14.0
87	9.0
88	4.5
89	3.0
90	2.0
91	4.0
92	6.5
93	6.0
94	4.5
95	6.5
96	7.5
97	4.0
98	1.0
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05125948995215588
2	5.788762275794001E-5
3	0.0019102915510120202
4	0.12477677085473969
5	0.013574647536736931
6	0.0025181115899703906
7	0.00480467268890902
8	0.009377794886786282
9	0.021649970911469564
10-11	0.017843859715135007
12-13	0.018610870716677715
14-15	0.027641339866916352
16-17	0.0354127532221698
18-19	0.03677311235698139
20-21	0.02169338662853802
22-23	0.023676037707997463
24-25	0.02192493711956978
26-27	0.020636937513205614
28-29	0.020550106079068705
30-31	0.02438516108678223
32-33	0.024949565408672145
34-35	0.028914867567591037
36-37	0.031158012949461213
38-39	0.03198291157376185
40-41	0.03463127031493761
42-43	0.03128826010066658
44-45	0.029320080926896617
46-47	0.03709149428215006
48-49	0.030448889570676447
50-51	0.023270824348691883
52-53	0.02872673279362773
54-55	0.031186956760840177
56-57	0.025181115899703906
58-59	0.027699227489674294
60-61	0.03872681962506187
62-63	0.047033693490826256
64-65	0.040448976402110585
66-67	0.037048078565081605
68-69	0.02916088996431228
70-71	0.03532592178803289
72-73	0.03891495439902517
74-75	0.024370689181092742
76-77	0.020868488004237375
78-79	0.02800313750915348
80-81	0.02452988014367708
82-83	0.03519567463682753
84-85	0.021664442817159048
86-87	0.030969878175497907
88-89	0.029812125720339106
90-91	0.030564664816192324
92-93	0.02626650882641528
94-95	0.02117239802371656
96-97	0.01813329782892471
98-99	0.023994419633166134
100-101	0.023806284859202827
102-103	0.025731048315904333
104-105	0.02302480195197064
106-107	0.014949478577238006
108-109	0.017207095864797666
110-111	0.02030408368234746
112-113	0.013603591348115902
114-115	0.014008804707421482
116-117	0.012851052252262681
118-119	0.007554334769911171
120-121	0.005528267973383271
122-123	0.003994245970297861
124-125	0.05725085890760267
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	3454970.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.33668005120548
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.57859837907665	42.015106355493934
2	13.468852648428904	15.175808846227415
3	5.057239754718881	8.547242940115035
4	2.37629765675659	5.354908831805013
5	1.3163153319514949	3.707841785132384
6	0.7836913765755302	2.649034220461466
7	0.5231880619949064	2.063227491865211
8	0.34627142324010274	1.5606225905562578
9	0.26848035345634647	1.3612762595434276
>10	1.1866741429146352	11.617142761560583
>50	0.062485041172159674	2.399232360983637
>100	0.030768653637561774	2.9575293526100057
>500	7.236575075671464E-4	0.2506677967947333
>1k	4.1351856874456146E-4	0.3403584068509097
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.7887622757940014E-5
2	2.8943811378970007E-5	0.0	0.0	0.0	5.7887622757940014E-5
3	2.8943811378970007E-5	0.0	0.0	0.0	5.7887622757940014E-5
4	2.8943811378970007E-5	0.0	0.0	0.0	5.7887622757940014E-5
5	8.683143413691001E-5	0.0	0.0	0.0	8.683143413691001E-5
6	8.683143413691001E-5	0.0	0.0	0.0	1.1577524551588003E-4
7	1.1577524551588003E-4	0.0	0.0	5.7887622757940014E-5	1.1577524551588003E-4
8	1.1577524551588003E-4	0.0	0.0	5.7887622757940014E-5	1.1577524551588003E-4
9	1.1577524551588003E-4	0.0	0.0	8.683143413691001E-5	1.1577524551588003E-4
10-11	1.1577524551588003E-4	0.0	0.0	1.0130333982639501E-4	1.1577524551588003E-4
12-13	1.4471905689485002E-4	0.0	0.0	1.7366286827382002E-4	3.4732573654764004E-4
14-15	1.4471905689485002E-4	0.0	0.0	1.7366286827382002E-4	4.7757288775300506E-4
16-17	1.7366286827382002E-4	0.0	0.0	1.7366286827382002E-4	6.078200389583701E-4
18-19	2.1707858534227503E-4	0.0	0.0	2.1707858534227503E-4	7.670110015427052E-4
20-21	2.6049430241073003E-4	0.0	0.0	2.4602239672124503E-4	9.117300584375553E-4
22-23	2.6049430241073003E-4	0.0	0.0	3.6179764223712505E-4	9.262019641270402E-4
24-25	4.0521335930558005E-4	0.0	0.0	4.6310098206352006E-4	9.262019641270402E-4
26-27	4.0521335930558005E-4	0.0	0.0	7.525390958532201E-4	0.00101303339826395
28-29	4.775728877530051E-4	0.0	0.0	0.0022286734761806904	0.0010419772096429201
30-31	5.209886048214601E-4	0.0	0.0	0.008133210997490571	0.0010564491153324052
32-33	5.209886048214601E-4	0.0	0.0	0.020752712758721495	0.0010998648324008601
34-35	6.367638503373401E-4	0.0	0.0	0.03814794339748247	0.0011577524551588001
36-37	7.235952844742501E-4	0.0	0.0	0.06442892412958723	0.0011577524551588001
38-39	8.393705299901302E-4	0.0	0.0	0.10780122548097379	0.0011722243608482852
40-41	8.683143413691002E-4	0.0	0.0	0.15966853547208804	0.0011866962665377703
42-43	9.262019641270401E-4	0.0	0.0	0.21363427178817762	0.0011866962665377703
44-45	0.0010709210210218902	0.0	0.0	0.27354796134264553	0.0012156400779167404
46-47	0.00112880864377983	0.0	0.0	0.3430709962749315	0.0012445838892957103
48-49	0.0012735277006746802	0.0	0.0	0.42030755693971295	0.001259055794985195
50-51	0.0012735277006746802	0.0	0.0	0.5012055097439341	0.0013024715120536503
52-53	0.0012735277006746802	0.0	0.0	0.585735910876216	0.0014182467575695303
54-55	0.0012735277006746802	0.0	0.0	0.6779508939296144	0.0014761343803274703
56-57	0.0013893029461905602	0.0	0.0	0.7858678946561042	0.0015774377201538653
58-59	0.0013893029461905602	0.0	0.0	0.903640263157133	0.0016208534372223202
60-61	0.0014327186632590153	0.0	0.0	1.0299365841092687	0.0016497972486012903
62-63	0.0014761343803274703	0.0	0.0	1.1578827023100056	0.0017366286827382004
64-65	0.0015050781917064402	0.0	0.0	1.2974497607793989	0.0017366286827382004
66-67	0.0015340220030854103	0.0	0.0	1.454339690359106	0.0017511005884276853
68-69	0.0015484939087748954	0.0	0.0	1.6319533888861555	0.0017655724941171703
70-71	0.0015629658144643802	0.0	0.0	1.8280332390729876	0.0017800443998066554
72-73	0.0015629658144643802	0.0	0.0	2.0292940314966557	0.0019826510794594453
74-75	0.0015629658144643802	0.0	0.0	2.2357212942514693	0.0021852577591122352
76-77	0.0015629658144643802	0.0	0.0	2.4603686862693452	0.0022142015704912053
78-79	0.0015774377201538653	0.0	0.0	2.72016833720698	0.0022286734761806904
80-81	0.0017366286827382004	0.0	0.0	2.9995050608254195	0.0022576172875596605
82-83	0.0017366286827382004	0.0	0.0	3.2923006567350805	0.0023589206273860555
84-85	0.0018089882111856255	0.0	0.0	3.586311892722658	0.0023878644387650256
86-87	0.0018668758339435653	0.0	0.0	3.902971661114279	0.0025036396842809056
88-89	0.0019102915510120205	0.0	0.0	4.25814117054562	0.0026338868354862707
90-91	0.00205501060790687	0.0	0.0	4.639779795483029	0.0026338868354862707
92-93	0.0020839544192858403	0.0	0.0	5.036744168545603	0.0026917744582442104
94-95	0.0020839544192858403	0.0	0.0	5.439526247695349	0.0026917744582442104
96-97	0.0020839544192858403	0.0	0.0	5.860123242748852	0.003125931628928761
98-99	0.0021852577591122352	0.0	0.0	6.30836157766927	0.0034153697427184606
100-101	0.00225761728755966	0.0	0.0	6.807425245371161	0.0034153697427184606
102-103	0.0023299768160070853	0.0	0.0	7.33489726394151	0.003487729271165886
104-105	0.0024891677785914205	0.0	2.8943811378970007E-5	7.864742675044935	0.0035311449882343407
106-107	0.0025470554013493603	0.0	2.8943811378970007E-5	8.402533162371888	0.0035311449882343407
108-109	0.0026049430241073005	0.0	2.8943811378970007E-5	8.959527868548786	0.0035311449882343407
110-111	0.0026338868354862707	0.0	2.8943811378970007E-5	9.556450562522974	0.0036179764223712506
112-113	0.0026628306468652403	0.0	2.8943811378970007E-5	10.190348975533796	0.0036469202337502207
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	7610	0.0	41.50689	1
GGTATCA	4760	0.0	38.938786	1
ATCAACG	9765	0.0	32.028267	3
TCAACGC	9735	0.0	32.005505	4
CAACGCA	9690	0.0	31.986198	5
TATCAAC	10015	0.0	31.4635	2
AACGCAG	10145	0.0	30.725769	6
ACGCAGA	11545	0.0	26.590693	7
CGCAGAG	11905	0.0	25.78735	8
GTACATG	12000	0.0	25.080667	1
TACATGG	12100	0.0	23.826612	2
ACATGGG	11685	0.0	23.553726	3
GCAGAGT	13245	0.0	21.920692	9
CATGGGG	8220	0.0	21.742447	4
AGAGTAC	11505	0.0	20.970234	10-11
GTACCGT	415	2.910383E-11	20.067974	6
GAGTACT	6005	0.0	18.875288	12-13
AGTACTT	6795	0.0	18.6072	12-13
CAGAGTA	12485	0.0	18.371088	10-11
GTACTTT	6495	0.0	17.359676	14-15
>>END_MODULE